A5019717373- Microbial Community Ecology and Physiology
- Polar Research and Ecology
- Thermoregulation and physiological responses
- Occupational Health and Performance
- Aquaculture Nutrition and Growth
- Fatty Acid Research and Health
- Neonatal Respiratory Health Research
- Marine Bivalve and Aquaculture Studies
- Psychological Testing and Assessment
- Genomics and Phylogenetic Studies
- Personality Disorders and Psychopathology
- Metabolomics and Mass Spectrometry Studies
- Meat and Animal Product Quality
- Exercise and Physiological Responses
- Plant Disease Resistance and Genetics
- Sports Performance and Training
- Microbial Metabolic Engineering and Bioproduction
- Tryptophan and brain disorders
- Cannabis and Cannabinoid Research
- Stress Responses and Cortisol
- Suicide and Self-Harm Studies
- Legume Nitrogen Fixing Symbiosis
- Pharmacological Effects and Assays
- Antioxidant Activity and Oxidative Stress
- Algal biology and biofuel production
University of Tasmania
2016-2025
Vanderbilt University Medical Center
2019-2025
Qatar Orthopaedic and Sports Medicine Hospital
2016-2024
Nashville Oncology Associates
2022-2023
Pacific University
2001-2023
Duke University Hospital
2023
Duke Medical Center
2023
Florida College
2021-2022
University of Florida
2021-2022
Pulmonary and Allergy Associates
2020-2022
The bacterial populations associated with sea ice sampled from Antarctic coastal areas were investigated by use of a phenotypic approach and phylogenetic based on genes encoding 16S rRNA (16S rDNA). diversity bacteria was also compared the seawater underlying ice. Psychrophilic (optimal growth temperature, < or = 15 degrees C; no occurring at 20 C) found to be significantly enriched in samples possessing platelet bottom diatom assemblages, 2 9 distinct (average, 5.6 +/- 1.8) psychrophilic...
ABSTRACT Lung organogenesis requires precise timing and coordination to effect spatial organization function of the parenchymal cells. To provide a systematic broad-based view mechanisms governing dynamic alterations in cells over crucial periods development, we performed single-cell RNA-sequencing time-series yielding 102,571 epithelial, endothelial mesenchymal across nine time points from embryonic day 12 postnatal 14 mice. Combining computational fate-likelihood prediction with RNA situ...
Abstract Large-scale changes in the structure and cellular makeup of distal lung are a hallmark pulmonary fibrosis (PF), but spatial contexts that contribute to disease pathogenesis have remained uncertain. Using image-based transcriptomics, we analyzed gene expression 1.6 million cells from 35 unique lungs. Through complementary cell-based innovative cell-agnostic analyses, characterized localization PF-emergent cell types, established molecular basis classical PF histopathologic features...
A polyphasic taxonomic study was performed to characterize dissimilatory iron-reducing strains mostly isolated from Antarctic sea ice. The were samples of congelated (land-fast) ice, grease and ice algal biomass collected the coastal areas Vestfold Hills in eastern Antarctica (68°S 78°E). facultatively anaerobic, motile, rod shaped, capable anaerobic growth either by fermentation carbohydrates or respiration, utilized a variety electron acceptors, including nitrate, ferric compounds,...
Several orange- and yellow-pigmented, halophilic, strictly aerobic, chemoheterotrophic, Gram-negative strains were isolated during investigations of maritime Antarctic habitats, including coastal fast sea-ice brine algae, crustaceans quartz stone sublithic cyanobacterial biofilms. Isolates investigated in this study belonged to the marine clade family Flavobacteriaceae represented lineages that either distinct from species with validly published names or appeared be within existing genera. A...
A group of sea-ice-derived psychrophilic bacterial strains possessing the unusual ability to synthesize polyunsaturated fatty acids eicosapentaenoic acid (20:5.3) and arachidonic (20:4.6) belong Family Flavobacteriaceae ( Flexibacter-Bacteroides-Flavobacterium phylum), according 16S rRNA sequence analysis. Surprisingly, isolates were also found cluster closely moderately halophilic psychrotrophic species [ Flavobacterium ] gondwanense (sequence similarity 97-8-98-1 %). The whole-cell...
Semidwarfing genes have improved crop yield by reducing height, improving lodging resistance, and allowing plants to allocate more assimilates grain growth. In wheat (
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) novel 2019 (COVID-19) global pandemic has led to millions of cases and hundreds thousands deaths. While older adults appear at high risk for disease, hospitalizations deaths due SARS-CoV-2 among children have been relatively rare. Integrating single-cell RNA sequencing (scRNA-seq) developing mouse lung with temporally resolved immunofluorescence in human tissue, we found that expression Spike protein primer TMPRSS2 was highest...
To characterise the core temperature response and power output profile of elite male female cyclists during 2016 UCI Road World Championships. This may contribute to formulating environmental heat stress policies.Core was recorded via an ingestible capsule in 10, 15 team time trial (TTT), individual (ITT) road race (RR), respectively. Power heart rate were extracted from cycling computers. Ambient conditions direct sunlight hot (37°C±3°C) but dry (25%±16% relative humidity), corresponding a...
Sexual reproduction in animals and plants shares common elements, including sperm egg production, but unlike animals, little is known about the regulatory pathways that determine sex of plants. Here we use mutants gene silencing a fern species to identify core mechanism plant sexual differentiation. A key player differentiation phytohormone abscisic acid (ABA), which regulates ratio male hermaphrodite tissues during reproductive cycle. Our analysis shows Ceratopteris richardii, homologous...
Rapid biosynthesis of abscisic acid (ABA) in the leaf, triggered by a decrease cell volume, is essential for functional stomatal response. However, it not known whether rapid ABA also other plant tissues. Through application external pressure to flower, root, and leaf tissues, we test reduction volume can trigger increases levels across body two species, Solanum lycopersicum Passiflora tarminiana. Our results show that, contrast synthesis flower root tissue did significant, increase level...
Emerging evidence indicates that early life events can increase the risk for developing chronic obstructive pulmonary disease (COPD). Using an inducible transgenic mouse model NF-κB activation in airway epithelium, we found a brief period of inflammation during saccular stage (P3–P5) but not alveolar (P10–P12) lung development disrupted elastic fiber assembly, resulting permanent reduction function and COPD-like phenotype progressed through 24 months age. Neutrophil depletion prevented...
Determining how alveoli are formed and maintained is critical to understanding lung organogenesis regeneration after injury. To study the cellular dynamics of this stage development, we have used scanned oblique-plane illumination microscopy living slices observe alveologenesis in real time at high resolution over several days. Contrary prevailing notion that occurs by airspace subdivision via ingrowing septa, find form ballooning epithelial outgrowth supported contracting mesenchymal ring...
Direct analysis of membrane lipids by liquid chromatography-electrospray mass spectrometry was used to demonstrate the role unsaturation in ether adaptation Methanococcoides burtonii low temperature. A proteomics approach using two-dimensional chromatography-mass identify enzymes involved lipid biosynthesis, and a pathway for biosynthesis reconstructed from M. draft genome sequence. The major phospholipids were archaeol phosphatidylglycerol, phosphatidylinositol, hydroxyarchaeol...
As part of a general survey the biodiversity and inherent ecophysiology bacteria associated with coastal Antarctic sea-ice diatom assemblages, eight strains were identified by 16S rRNA sequence analysis as belonging to genus Colwellia. The isolates non-pigmented, curved rod-like cells which exhibited psychrophilic facultative anaerobic growth possessed an absolute requirement for sea water. One isolate was able form gas vesicles. All synthesized ω3 polyunsaturated fatty acid (PUFA)...
Thirty eight strains of Antarctic bacteria were screened for the ability to produce polyunsaturated fatty acids (PUFA). Five contained eicosapentaenoic acid (20:5ω3) in range trace 3.3% total acids, and up 1.4 mg g -1 dry weight. Thirteen produced polyunsaturates including 18:2ω6, 18:3ω3, 18:4ω3 20:4ω6 7.0% acids. Although data set is currently small, proportion found PUFA's higher than that temperate marine (and similar recorded barophilic bacteria). This suggests environment has naturally...
Adults with chronic kidney disease (CKD) exhibit alterations in tryptophan metabolism, mainly via the kynurenine pathway, due to higher enzymatic activity induced by inflammation. Indoles produced gut-microflora are another group of metabolites related inflammation and conditions accompanying CKD. Disruptions metabolism have been associated various neurological psychological disorders. A high proportion CKD patients self-report symptoms depression and/or anxiety decline cognitive...
Summary In recent years the biosynthesis of auxin has been clarified with aid mutations in genes. However, we know little about effects these on seed‐filling stage seed development. Here investigate a key mutation garden pea, which results deficiency developing seeds. We exploit large size this model species, facilitates measurement compounds individual The small seeds reduced starch content and wrinkled phenotype at dry stage. phenotypic were fully reversed by introduction wild‐type gene as...
Land plants lose vast quantities of water to the atmosphere during photosynthetic gas exchange. In angiosperms, a complex network veins irrigates leaf, and it is widely held that density placement these determines maximum leaf hydraulic capacity thus rate. This theory largely based on interspecific comparisons has never been tested using vein mutants examine specific impact morphology plant relations. Here we characterize at Crispoid (Crd) locus in pea (Pisum sativum), which have altered...
A hallmark of idiopathic pulmonary fibrosis (IPF) and other interstitial lung diseases is dysregulated repair the alveolar epithelium. The Hippo pathway effector transcription factors YAP TAZ are implicated as essential for type 1 2 epithelial cell (AT1 AT2) differentiation in developing lung, yet aberrant activation YAP/TAZ a prominent feature epithelium IPF. In these studies, we sought to define functional role activity during regeneration. We demonstrated that Yap Taz were normally...