A5078996982- HER2/EGFR in Cancer Research
- Monoclonal and Polyclonal Antibodies Research
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Cancer Cells and Metastasis
- Methane Hydrates and Related Phenomena
- Cell Adhesion Molecules Research
- Polar Research and Ecology
- HIV Research and Treatment
- HIV/AIDS Research and Interventions
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Protist diversity and phylogeny
- Cancer Research and Treatments
- Lymphoma Diagnosis and Treatment
- Melanoma and MAPK Pathways
- HIV/AIDS drug development and treatment
- Chronic Lymphocytic Leukemia Research
- Bladder and Urothelial Cancer Treatments
- Urinary and Genital Oncology Studies
- Kruppel-like factors research
- Advanced MEMS and NEMS Technologies
- Endoplasmic Reticulum Stress and Disease
- Gene Regulatory Network Analysis
- Immunodeficiency and Autoimmune Disorders
Seagen (United States)
2020-2023
Gilead Sciences (United States)
2017-2018
Amgen (United States)
2009-2015
University of Washington
1993-2006
Seattle University
1997-1998
University of Wisconsin–Madison
1990
Several psychrophilic, gas vacuolate strains of the Cytophage-Flavobacterium-Bacteroides (CFB) phylogenetic group were isolated from sea ice and water Arctic Antarctic. The closest taxonomically defined species by 16S rRNA sequence analysis is 'Flectobacillus glomeratus'. However, 'Flc. glomeratus' phylogenetically distant Flectobacillus type species, Flc. major. On basis phenotypic, genotypic analyses we propose a new genus, Polaribacter, with three Polaribacter irgensii strain 23-P (ATCC...
As part of a general survey the biodiversity and inherent ecophysiology bacteria associated with coastal Antarctic sea-ice diatom assemblages, eight strains were identified by 16S rRNA sequence analysis as belonging to genus Colwellia. The isolates non-pigmented, curved rod-like cells which exhibited psychrophilic facultative anaerobic growth possessed an absolute requirement for sea water. One isolate was able form gas vesicles. All synthesized ω3 polyunsaturated fatty acid (PUFA)...
ABSTRACT The development of viral diversity during the course human immunodeficiency virus type 1 (HIV-1) infection may significantly influence pathogenesis. paradigm for HIV-1 evolution is based primarily on studies male cohorts in which individuals were presumably infected with a single variant subtype B HIV-1. In this study, we evaluated sequence information V1, V2, and V3 portions clade A envelope genes obtained from peripheral blood cervical secretions three women genetically...
Several strains of a novel heterotrophic gas vacuolate bacterium were isolated from antarctic marine waters. The results phylogenetic analyses in which 16S ribosomal DNA sequencing was used, coupled with pheno-typic tests, indicated that strain 34-PT (T = type strain) belongs to new genus and species the beta subgroup Proteobacteria, for name Polaromonas vacuolata is proposed. Although other four studied probably belong this species, DNA-DNA hybridization tests not conducted. closest...
A gas vacuolate bacterium, designated strain 37 T , was isolated from a sea ice core collected Point Barrow, Alaska, USA. Cells of were large (6–14 μm in length), rod-shaped, contained vacuoles two distinct morphologies, and grew well at NaCl concentrations 1–10 % temperatures −12 to 10 °C. The DNA G+C content 40 mol%. Whole-cell fatty acid analysis showed that 16 : 1 ω 7 c comprised 67 the total content. Phylogenetic 16S rRNA gene sequences indicated this bacterium closely related members...
SGN-B7H4V is a novel investigational vedotin antibody-drug conjugate (ADC) comprising B7-H4-directed human monoclonal antibody conjugated to the cytotoxic payload monomethyl auristatin E (MMAE) via protease-cleavable maleimidocaproyl valine citrulline (mc-vc) linker. This linker-payload system has been clinically validated in multiple Food and Drug Administration approved agents including brentuximab vedotin, enfortumab tisotumab vedotin. B7-H4 an immune checkpoint ligand with elevated...
SGN-CD228A is an investigational antibody-drug conjugate (ADC) directed to melanotransferrin (CD228, MELTF, MFI2, p97), a cell-surface protein first identified in melanoma. consists of humanized antibody, hL49, with high specificity and affinity for CD228 that stably conjugated 8 molecules the clinically validated microtubule-disrupting agent monomethyl auristatin E (MMAE) via novel glucuronide linker. We performed comprehensive IHC studies, which corroborated published RNA sequencing data...
Psychrophilic, gas vacuolate, heterotrophic bacteria indigenous to sea ice communities in Antarctica have been isolated. Phylogenetic analysis of representative members these shows that they belong the alpha, beta, and gamma Proteobacteria Flavobacteria-Cytophaga group. This is first report vacuolate from beta groups.
Depletion of two nonessential amino acids, asparagine (Asn) and glutamine (Gln), occurred during a fed-batch production process with CHO cell line expressing recombinant antibody. This depletion coincided growth suppression the onset stationary phase. Experimental withdrawal Asn led to cycle arrest A in G0/G1 On mechanistic level, either or Gln stimulated amino-acid response (AAR) pathway, indicating that acids can induce AAR this line. Compared an essential acid, leucine (Leu), induced...
Prosthecobacter fusiformis is morphologically similar to caulobacters; however, it lacks a dimorphic life cycle. To determine the relatedness of genus caulobacters and other prosthecate members alpha subgroup Proteobacteria (alpha-Proteobacteria), we isolated sequenced 16S rRNA genes from four strains. Surprisingly, results phylogenetic analyses placed fusiform in deeply rooted division Bacteria that was most closely affiliated with Planctomyces-Chlamydia group only distantly related...
Abstract The antibody-drug conjugate enfortumab vedotin (EV; AGS22C3E) targets Nectin-4 expressing tumor cells by delivering MMAE, a potent microtubule disrupting agent, to induce cell death. EV has demonstrated single agent activity and encouraging (71% ORR) when combined with pembrolizumab (anti-PD-1) in the 1L setting of cis-ineligible metastatic urothelial carcinoma (mUC) (EV-103, NCT03288545). Here we that may promote multiple mechanisms action including bystander killing hallmarks...
Diversity among global isolates of HIV-1 presents a formidable challenge for vaccine development. As distinct clades the virus are recognized, it will be important to monitor their geographic distribution and divergence. In this study, we characterized subtypes from 17 seropositive individuals in Nairobi Mombasa, Kenya. Seventy-one percent viruses were clade A 29% D. The most divergent isolate our survey, Q45-CxA, grouped closely with two other taxa that previously reported as having no...
Heterotrophic bacteria were enumerated in north polar sea ice cores obtained near Point Barrow, Alaska. Highest concentrations of total and viable found the layer containing microbial community identified by maximum chlorophyll a content. Gas vacuolate also ice, discovery which is consistent with their recent report from antarctic communities. The gas comprised 0.2% or less isolated cores, lower than reported for most samples. Most isolates pigmented pink, orange, yellow. An core nearby...
A gas-vacuolate bacterium, strain 174(T), was isolated from a sea-ice core collected Point Barrow, Alaska, USA. Comparative analysis of 16S rRNA gene sequences showed that this bacterium most closely related to Psychromonas ingrahamii 37(T), with similarity >99 %. However, 174(T) could be clearly distinguished species by DNA-DNA hybridization; relatedness values determined two different methods between and P. 37(T) were 58.4 55.7 % those antarctica DSM 10704(T) 46.1 33.1 %, which are well...
The simian immunodeficiency virus (SIV) Mne envelope undergoes genetic changes that alter tropism, syncytium-inducing capacity, and antigenic properties of the emerging variant population during course an infection. Here we investigated whether mutations in SIVMne also influence coreceptor usage. data demonstrate infecting macrophage-tropic clone as well variants are selected disease progression all recognize both CCR5 Bob (GPR15) but not Bonzo (STRL33), CXCR4, or CCR3. Although it remains...
<h3>Background</h3> Enfortumab vedotin (EV) is a first-in-class Nectin-4-directed antibody-drug conjugate (ADC) with demonstrated improved overall survival in patients previously treated advanced-stage urothelial carcinoma.<sup>1</sup> EV comprised of fully human monoclonal antibody conjugated to the microtubule-disrupting agent monomethyl auristatin E (MMAE) by protease cleavable maleimidocaproyl-valine-citrulline linker. has multifaceted mechanism action. Previously, we that induces...
Bruton's tyrosine kinase (BTK) is a clinically validated target for B-cell leukemias and lymphomas with FDA-approved small-molecule inhibitors ibrutinib acalabrutinib. Tirabrutinib (GS-4059/ONO-4059, Gilead Sciences, Inc., Foster City, CA) second-generation, potent, selective, irreversible BTK inhibitor in clinical development lymphoid malignancies, including chronic lymphocytic leukemia (CLL) diffuse large lymphoma (DLBCL). An accurate pharmacodynamic assay to assess tirabrutinib coverage...
GS-9674 is a non-steroidal Farnesoid X Receptor (FXR) agonist in development for the treatment of nonalcoholic steatohepatitis (NASH) and cholestatic liver disorders. Oral increased plasma fibroblast growth factor 19 (FGF19), decreased 7-α-hydroxy-4-cholesten-3-one (C4), improved histology rodent primate models NASH. This first-in-human study evaluated safety, pharmacokinetics (PK), pharmacodynamic (PD) effects healthy volunteers. Data on PD markers (FGF19 C4), PK/PD PD/PD relationships,...
<h3>Background</h3> Tisotumab vedotin (TV) is an investigational antibody-drug conjugate composed of a tissue factor (TF)-directed human monoclonal antibody covalently linked to the microtubule-disrupting agent monomethyl auristatin E (MMAE) via protease-cleavable linker. TV demonstrated single activity (24% objective response rate [ORR]) in previously treated recurrent or metastatic cervical cancer (NCT03438396) where currently, there no standard care and ORRs are typically less than 15%...
In flow cytometry, different cell types are usually selected or "gated" by a series of 1- 2-dimensional geometric subsets the measurements made on each cell. This is easily accomplished in commercial cytometry packages but it difficult to work computationally with results this process. The ability retrieve and both them raw data critical; our experience points importance bioinformatics tools that will allow us examine gating robustness, combine manual automated gating, perform exploratory...