A5087784820- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Plant-Microbe Interactions and Immunity
- Gastric Cancer Management and Outcomes
- Advanced Mathematical Physics Problems
- Renal Diseases and Glomerulopathies
- Esophageal and GI Pathology
- Gastrointestinal motility and disorders
- Chromosomal and Genetic Variations
- Metastasis and carcinoma case studies
- Electrolyte and hormonal disorders
- Heat shock proteins research
- Heart Rate Variability and Autonomic Control
- Abdominal vascular conditions and treatments
- Autoimmune Bullous Skin Diseases
- Thermoregulation and physiological responses
- Neurological Disease Mechanisms and Treatments
- Gastrointestinal disorders and treatments
- Medicinal Plants and Bioactive Compounds
- Stability and Controllability of Differential Equations
- Neurological Disorders and Treatments
- Vitamin C and Antioxidants Research
- Dialysis and Renal Disease Management
- Transgenic Plants and Applications
- Plant Pathogenic Bacteria Studies
Konan Kosei Hospital
2022-2024
Nagoya University
2006-2023
Amino Up (Japan)
2013-2023
Yamaguchi University
1981-2023
Japanese Red Cross Nagoya Daini Hospital
2014
University of Shizuoka
2009-2014
Hokkaido University
2003-2012
Ministry of Agriculture and Cooperatives
2010
Kyoto University
2004-2007
Nippon Soda (Japan)
2004-2005
RNA silencing (RNAi) induced by virus-derived double-stranded (dsRNA), which is in a sense regarded as pathogen-associated molecular pattern (PAMP) of viruses, general plant defense mechanism. To counteract this defense, viruses express suppressors (RSSs), many bind to dsRNA and attenuate RNAi. We showed that the tobacco calmodulin-like protein, rgs-CaM, counterattacked viral RSSs binding their dsRNA-binding domains sequestering them from inhibiting Autophagy-like protein degradation seemed...
Double-stranded (ds) RNAs and imperfect hairpin of endogenous genes trigger post-transcriptional gene silencing (PTGS) are cleaved by a Dicer-like nuclease into small interfering (siRNAs) microRNs (miRNAs), respectively. Such (siRNAs miRNAs) then guide an RNA-induced complex (RISC) for sequence-specific RNA degradation. While PTGS serves as antiviral defense in plants, many plant viruses encode suppressors counter defense. Here we demonstrate that the suppressor (2b) severe strain (CM95R)...
Most commercial Glycine max (soybean) varieties have yellow seeds because of loss pigmentation in the seed coat. It has been suggested that inhibition coat G. may be controlled by homology-dependent silencing chalcone synthase (CHS) genes. Our analysis CHS mRNA and short-interfering RNAs provide clear evidence results from posttranscriptional rather than transcriptional Furthermore, we show mottling symptoms present on plants infected with some viruses can caused suppression gene (PTGS) a...
Petunia hybrida 'Red Star' is a variety whose flowers exhibit star-type red and white bicolor pattern. We analyzed the mRNA levels of six genes involved in anthocyanin biosynthesis. Only level chalcone synthase (CHS) was depressed unpigmented flower sectors. Both transcriptional activity accumulation short interfering RNA CHS sectors were detected. Viral infection blocked generation CHS-silenced These results indicate that sequence-specific degradation primary cause formation flowers.
Summary Gene silencing through transcriptional repression can be induced by targeting double‐stranded RNA (dsRNA) to a gene promoter. It has been reported that transgene was silenced dsRNA the promoter, and state inherited progeny plant even after removal of inducer from cells. In contrast, no produced harbors endogenous promoter‐targeting dsRNA. Here, we show heritable promoters in petunia tomato plants, using Cucumber mosaic virus (CMV)‐based vector. We found efficient genes depends on...
Reactive oxygen species (ROS) are proposed to function as diffusible signaling molecules in plant immune response. Rice respiratory burst oxidase homologs (Osrboh genes) play a role ROS generation. We examined rice responses of four Osrboh homologs, OsrbohA, OsrbohB, OsrbohD, and OsrbohE. OsrbohA OsrbohD transcripts were induced after inoculation with an incompatible N1141 strain Acidovorax avenae, whereas OsrbohaB OsrbohE mRNA levels did not obviously change even the strain. genes...
Serotonin (5-hydroxytryptamine: 5-HT) affects numerous functions in the gut, such as secretion, muscle contraction, and enteric nervous activity, therefore to clarify details of 5-HT's actions leads good therapeutic strategies for gut functional disorders. The role interstitial cells Cajal (ICC), pacemaker cells, has been recognised relatively recently. We thus investigated 5-HT on ICC activity. Muscle preparations with myenteric plexus were isolated from murine ileum. Spatio-temporal...
To explore the electrophysiological properties of interstitial cells Cajal (ICCs) and fibroblast-like (FLCs), we developed a new preparation by treating murine small intestine with collagenase. This thin muscle layer contained at least two types around enteric nerve bundles, cluster smooth displayed rhythmic contraction. We morphologically identified ICCs FLCs conducted patch clamp experiments on each type cell. The c-kit-positive CD34-negative showed spontaneous potential fluctuations,...
We developed a novel RNA virus vector based on the Cucumber mosaic (CMV), which is able to efficiently induce gene silencing in plants. manipulated 2 of CMV Y strain, whose genome consists tripartite components, and introduced restriction sites for cloning foreign sequence into vector. To evaluate (designated CMV2-A1) terms ability silencing, we cloned portions green fluorescent protein (GFP) cDNA or Cauliflower (CaMV) 35S promoter sequences inoculated infectious transcripts Nicotiana...
Background: Good sleep is essential for a healthy, active life that supports disease prevention. Sleep debts caused by deprivation, irregular sleep, and insomnia increase the risk of metabolic syndrome, mood disorders, dementia, cancer. Today's lifestyles in developed countries are prone to debts, which has led social interventions aim reduce sleep-related health problems. ETAS®50 standardized extract Asparagus officinalis stem, been reported improve quality, maintain rhythms, relieve mental...
Gastrointestinal (GI) motility is well organized. GI muscles act as a functional syncytium to achieve physiological functions under the control of neurones and pacemaker cells, which generate basal spontaneous electrical activity. To date, it unclear how activities are coupled, especially within micrometre range. Here, using microelectrode array, we show spatio-temporal analysis The muscle preparations were isolated from guinea-pig stomach, fixed in chamber with an array 8 x planar...
Enzyme-treated asparagus extract (ETAS) has been developed as a novel anti-stress functional food ingredient that is produced from asparagus. Two human intervention trials with ETAS were conducted in healthy adult male volunteers. Study 1 was randomized, double-blind, placebo-controlled study to assess the effects of on expression heat shock protein 70 (HSP70) mRNA blood and autonomic nervous system (ANS). The group showed tendency enhance HSP70 level compared placebo group. Several ANS...
A novel 5-hydroxymethyl-2-furfural (HMF; 1) derivative, which is named asfural (compound 2), was isolated from enzyme-treated asparagus extract (ETAS) along with HMF (1) as a heat shock protein 70 (HSP70) inducible compound. The structure of compound 2 elucidated on the basis its spectroscopic data HREIMS and NMR, whereas absolute configuration determined using chiral HPLC analysis, compared to two synthesized compounds, (S)- (R)-asfural. As result, derived ETAS assigned...
A novel enzyme-treated asparagus extract (ETAS) has been developed as a functional material produced from stem. Studies were conducted to determine the effect of ETAS on heat shock protein 70 (HSP70) expression and alleviation stress. HeLa cells treated with ETAS, HSP70 mRNA levels measured using reverse transcription-polymerase chain reaction (RT-PCR) assay an enzyme-linked immunosorbent (ELISA), respectively. showed significant increases in at more than 0.125 mg/mL 1.0 mg/mL. The...
summary Infection of cultured rice cells with an incompatible strain Pseudomonas avenae induces a hypersensitive reaction the host, while compatible infection produces no such reaction. The induction H 2 O generation in by P. precedes cell death. To examine distribution sites, were incubated following cerium solution. Detection product, Ce(OH) OOH, was performed using energy disperse X‐ray microanalysis (EDX) fitted variable‐pressure scanning electron microscope (VP‐SEM). We determined that...
Transmural nerve stimulation (TNS) of the right atria in vitro produced positive and negative chronotropic responses rats guinea pigs. The component appeared only temporarily soon after cessation TNS was usually masked by component. were accompanied an increase decrease contractile force, respectively. Atenolol (3 X 10(-6) M) decreased atropine (10(-6) potentiated TNS-induced response. In presence both atenolol atropine, accelerated heart rate markedly pigs slightly those rats. acceleration...
Flutamide, when administered subcutaneously to female rats at doses of 3, 10, or 30 mg/kg/day during late pregnancy (gestational days 16-21), significantly and dose-dependently decreased anogenital distance (AGD) the male offspring in each dose group compared controls. Significant delays preputial separation were found males a mg/kg, but body weight gain was not inhibited. Cryptorchidism absence prostate gland seminal vesicles ≥ 10 testicular hypoplasia mg/kg. Hypospadias noted all groups...