A5081830609- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Toxoplasma gondii Research Studies
- Pancreatic function and diabetes
- RNA regulation and disease
- Prostate Cancer Treatment and Research
- Parasitic Infections and Diagnostics
- Vector-borne infectious diseases
- Cell death mechanisms and regulation
- Intracranial Aneurysms: Treatment and Complications
- Pneumonia and Respiratory Infections
- Herpesvirus Infections and Treatments
- Toxin Mechanisms and Immunotoxins
- Coccidia and coccidiosis research
- Ubiquitin and proteasome pathways
- Antibiotics Pharmacokinetics and Efficacy
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- HIV Research and Treatment
- Cancer, Lipids, and Metabolism
- Cellular transport and secretion
- Legionella and Acanthamoeba research
- Nuclear Structure and Function
- Cytomegalovirus and herpesvirus research
- Pancreatitis Pathology and Treatment
Hiroshima University
2016-2025
Kanazawa University
2024-2025
Keio University
2025
Astellas Pharma (Japan)
2016-2025
Hirosaki University
2023
Astellas Pharma (China)
2023
Kohnan Hospital
2011-2021
Sapporo Medical University
2017-2020
Institute of Vegetable and Floriculture Science
2018
Aomori Prefectural Central Hospital
2015-2016
Eukaryotic cells deal with accumulation of unfolded proteins in the endoplasmic reticulum (ER) by protein response, involving induction molecular chaperones, translational attenuation, and ER-associated degradation, to prevent cell death.Here, we found that autophagy system is activated as a novel signaling pathway response ER stress.Treatment SK-N-SH neuroblastoma stressors markedly induced formation autophagosomes, which were recognized at ultrastructural level.The green fluorescent...
Adipose tissue plays a central role in maintaining metabolic homeostasis under normal conditions. Metabolic diseases such as obesity and type 2 diabetes are often accompanied by chronic inflammation adipose dysfunction. In this study, we observed that endoplasmic reticulum (ER) stress the inflammatory response occurred of mice fed high-fat diet for period 16 weeks. After weeks feeding, ER markers increased tissue. We found is induced free fatty acid (FFA)-mediated reactive oxygen species...
To avoid excess accumulation of unfolded proteins in the endoplasmic reticulum (ER), eukaryotic cells have signaling pathways from ER to cytosol or nucleus. These processes are collectively termed stress response. Double stranded RNA activated protein kinase (PKR)-like (PERK) is a major transducer response and directly phosphorylates eIF2α, resulting translational attenuation. Phosphorylated eIF2α specifically promotes translation transcription factor ATF4. ATF4 plays important roles...
The c-Jun N-terminal protein kinase (JNK) signaling pathway is implicated in neuronal apoptosis. mechanism by which activated JNK induces apoptosis strongly linked to mitochondrial apoptogenic proteins, although the molecular machinery downstream of has not been precisely elucidated. Our study examined relevance proapoptotic Bcl-2 family members JNK-mediated after transient focal cerebral ischemia (tFCI), which, when induced 60 min middle artery (MCA) occlusion, elevated levels activity and...
The endoplasmic reticulum (ER), which plays a role in apoptosis, is susceptible to oxidative stress. Because superoxide produced the brain after ischemia/reperfusion, injury this organelle may be implicated ischemic neuronal cell death. Activating transcription factor-4 (ATF-4) and C/EBP-homologous protein (CHOP), both of are involved induced by severe ER Using wild-type human copper/zinc dismutase transgenic rats, we observed induction these molecules global cerebral ischemia compared them...
Endoplasmic reticulum (ER) stress transducers IRE1 (inositol requiring 1), PERK (PKR-like endoplasmic kinase), and ATF6 (activating transcription factor 6) are well known to transduce signals from the ER cytoplasm nucleus when unfolded proteins accumulate in ER.Recently, we identified OASIS (old astrocyte specifically induced substance) as a novel transducer expressed astrocytes.We report here that BBF2H7 (BBF2 human homolog on chromosome 7), an ER-resident transmembrane protein with bZIP...
<i>Background:</i> Cerebral hyperperfusion is a potential complication of superficial temporal artery-middle cerebral artery (STA-MCA) anastomosis for moyamoya disease, but the optimal postoperative management has not been determined. Aggressive blood pressure lowering controversial because risk ischemic complications. <i>Objective:</i> To establish protocol to prevent symptomatic in disease. <i>Methods:</i>...
The optimal treatment sequence of approved therapies for castration-resistant prostate cancer (PC) is unclear. This study assessed real-world patient characteristics, patterns, and effectiveness in patients with PC Japan. Using data from the Japan Study Group Prostate Cancer registry (2016-2018), this retrospective included ≥1 record of: primary androgen-deprivation therapy hormone-sensitive clinical progression to during therapy. outcomes were duration. Other overall survival (OS),...
ABSTRACT Nocardioides sp. strain KP7 grows on phenanthrene but not naphthalene. This organism degrades via 1-hydroxy-2-naphthoate, o -phthalate, and protocatechuate. The genes responsible for the degradation of to -phthalate ( phd ) were found by Southern hybridization reside chromosome. A 10.6-kb DNA fragment containing eight was cloned sequenced. phdA , phdB phdC phdD genes, which encode α β subunits oxygenase component, a ferredoxin, ferredoxin reductase, respectively, dioxygenase...
When unfolded or misfolded proteins accumulate in the endoplasmic reticulum (ER), protein response (UPR) signals are transmitted from ER to nucleus and cytoplasm facilitate folding. OASIS (old astrocyte specifically induced substance) is an stress transducer astrocytes, a membrane-bound transcription factor that activates genes response. ER, cleaved at membrane release its cytoplasmic domain, which then enters target genes. Here, we showed processed by Site-1 -2 proteases (S1P S2P), enzymes...
Although the endoplasmic reticulum (ER) is implicated in neuronal degeneration some situations, its role delayed cell death (DND) after ischemia remains uncertain. The authors speculated that ER stress involved DND, it reduced by ischemic preconditioning, and reduction preconditioning due to molecular chaperone induction. phosphorylation status of eukaryotic initiation factor 2α (eIF2α) RNA-dependent protein kinase–like eIF2α kinase (PERK) was investigated rat hippocampus with without...
Using an isolated perfused rat pancreas preparation, the interrelationship between endocrine and exocrine portions of were studied. Addition exogenous insulin (1-20 mU/ml) to perfusing solution potentiated action cholecystokinin (CCK) (1 increase both pancreatic juice flow release enzyme, amylase. Raising glucose concentration in from 2.5 17.5 mM increased endogenous CCK-induced secretory response. Two lines evidence indicated that this effect on was mediated by release. First, addition...
1. Insulin (1 mu./ml.) potentiated the release of amylase from isolated pancreas rats perfused with erythrocyte‐containing medium and stimulated by 0‐5 m‐u. pancreozymin (Pz)/ml., whereas same concentration insulin failed to potentiate response evoked or 200 PZ/ml. 2. Intracellular measurement membrane potentials acinar cells preparations showed that simultaneously potentiates hyperpolarization in 3. These effects on PZ‐induced responses were inhibited ouabain (3 X 10(‐5) M). 4. The results...
OASIS is a member of the CREB/ATF family transcription factors and modulates cell- or tissue-specific unfolded protein response signalling. Here we show that this modulation has critical role in differentiation neural precursor cells into astrocytes. Cerebral cortices mice specifically deficient (Oasis−/−) contain fewer astrocytes more than those wild-type during embryonic development. Furthermore, astrocyte delayed primary cultured Oasis−/− cells. The factor Gcm1, which necessary for...
The Bad signaling pathway contributes to the regulation of apoptosis after a variety cell death stimuli, and plays key role in determining or survival. We have reported that overexpression copper/zinc superoxide dismutase (SOD1) reduces apoptotic transient focal cerebral ischemia (tFCI). However, both tFCI oxygen free radicals remain unknown. To clarify these issues, we used an vivo model SOD1 transgenic mice wild-type mice. Moreover, examine protein kinase A (PKA) tFCI, administered PKA...
The endoplasmic reticulum (ER), which plays important roles in apoptosis, is susceptible to oxidative stress. Because reactive oxygen species (ROS) are robustly produced the ischemic brain, ER damage by ROS may be implicated neuronal cell death. We induced global brain ischemia on wild-type and copper/zinc superoxide dismutase (SOD1) transgenic rats compared stress damage. Phosphorylated forms of eukaryotic initiation factor 2α (eIF2α) RNA-dependent protein kinase-like eIF2α kinase (PERK),...
The Akt signaling pathway contributes to regulation of apoptosis after a variety cell death stimuli. A novel proline-rich substrate (PRAS) was recently detected and found be involved in apoptosis. In our study, activation modulated by growth factors, treatment with nerve factor (NGF) reduced apoptotic ischemic injury. However, the role PRAS neuronal ischemia remains unknown. Phosphorylated (pPRAS) binding pPRAS/phosphorylated (pPRAS/pAkt) 14-3-3 (pPRAS/14-3-3) were detected, their expression...