Abstract Marine sponges are rich sources of bioactive compounds with a wide range structures and potential applications. This study aims to examine the in marine Amphimedon viridis Aaptos pernucleata by using GC-MS analysis ethanol extracts. The revealed that A. contained diethyl phthalate (30.73%), cholesterol (12.21%), desmosterol (3.25%), hexadecenoic acid methyl ester (3.44%), 9-octadecenoic (2.18%), gamma sitosterol (1.33%). major found were (28.86%), bis (2-ethylhexy) (18.71%), acid,...

Objective: The objective of this study was to determine the new bioactive compounds through gas chromatography–mass spectrometry analysis and cytotoxic activity two rodent tuber mutant plants against breast cancer cells (MCF-7).
 Methods: in were successfully increased by somaclonal variation using gamma rays irradiation technique. Further, cytotoxicity tested on cell line (MCF-7) performed 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide assay method.
 Results: This...

The gamma-ray irradiation has been used to increase the content in chemical compounds of Typhonium flagelliforme with anticancer activity. This study was determine new bioactive through 1H-NMR, 13C NMR spectra, and HR-TOF MS analysis isolated from Tipobio variety mutant plant were found fatty acid group which have potential as Two derivatives, 2-octenoic (1) 2-hexenoic (2) ethyl acetate extract rodent tuber (T. flagelliforme). structures identified based on spectroscopic evidence compared...

Typhonium flagelliforme is an Indonesian herbal plant used and applied traditionally to treat cancer diseases. Gamma rays have irradiated rodent tuber mutant at six doses gray in-crease the chemical compounds of anticancer activity. The effect isolated from ro-dent plants has never been studied published yet. Our study unveiled poten-tial stigmasterol as a remarkable cytotoxic agent significant contribution NMR spectroscopy, IR, Mass spectra, QTOF MS towards isolation identification this...

Abstract Superior mutant rodent tuber plant ( Typhoniumflagelliforme ) is a medicinal herb of Indonesia, which immensely useful for anticancer activity. Some studies reported that the leaves and tubers, conventionally parts plant, showed various anticancer, antimicrobial, antioxidant potential. This study aim to determine bioactive compounds superior plants through n -hexane ethyl acetate fractions using GC-MS analysis. Phytochemical characterization extracts was detected by qualitative A...

Abstract The combination of gamma-ray irradiation treatment and somaclonal variation may cause morphological changes in vitro culture rodent tuber. In shoots were irradiated with several doses. Rodent tuber plantlets the doses 20 Gy. subcultured up to 5 times then produced MV1 MV5. Plantlets MV4 MV5 acclimatized transplanted at greenhouse. purpose this study was analyze characteristics fourth mutant vegetative propagation (MV4) fifth (MV5) from variation. Subcultures performed regenerate...

Rodent tuber is a potential plant which can be developed as an anticancer drug. The rodent has low compound does not have economic value to utilized on industrial scale, particularly explore material was used 30 clones of Tuber Pekalongan increase its bioactive compounds using combination in vitro culture with gamma-ray irradiation. selection process been done until the 8th generation and obtained 14 somaclonal mutants that higher than control (non-mutant). This study aimed analyze genetic...

Abstract Rodent tuber ( Thyphonium flagelliforme ) is a herbal plant that has anticancer bioactive compounds. In vitro , conservation attemptto preserve germplasm under aseptic conditions. Conservation techniques are used to store and minimize somaclonal variation. This study aims identifysucrose’s effect on the minimum growth of rodent in culture obtain right sucrose concentration for extended shelf life. was plantlets from Bogor Pekalongan using ½AMS medium with 40, 50, 60, 70 g/L...

Typhonium flagelliforme Lodd. is rodent tuber as an herbal plant with significant potential a cancer drug raw material. GC-MS analysis revealed that the contained bioactive compounds anticancer agents, including stigmasterol, hexadecanoic acid, oleic and squalene. Rodent tubers are vegetatively propagated crop resulted in low genetic diversity. Gamma irradiation somaclonal variation responsible for changing of tissue culture. In several mutant clones Pekalongan accession, high levels...

Historically, Southeast Asian coastal communities have used Xylocarpus granatum (XC) to treat malaria, diarrhea, cholera, fever, and cancer. It is crucial develop bioactive substances highly effective cytotoxic agents that cause minimal side effects so new anticancer plant therapies can be developed. The purpose of this study evaluate the major minor compounds in XC leaves ethanol extracts from Ambon Region, East Indonesia, as well possible extract. cells are HEK293 human kidney MCF-7 breast...

Typhonium flagelliforme (T. flagelliforme) is an Indonesian rodent tuber plant traditionally used to treat cancer diseases. Although gamma-ray irradiation has been increase the content in chemical compounds of T. plants with anticancer activity ten times effective, specific effect isolated from mutant never reported yet. The potential cytotoxic agents were characterized via nuclear magnetic resonance spectroscopy, infrared and mass spectrometry as stigmasterol 7α-hydroxyl stigmasterol; their...

Abstract. Sianipar NF, Muflikhati Z, Assidqi K. 2024. Optimization design of tetra-primer ARMS-PCR using SNP lectin gene and in silico characterization protein rodent tuber (Typhonium flagelliforme) mutant Bogor accessions. Nusantara Bioscience 16: 201-209. Rodent plant flagelliforme (G.Lodd.) Blume) contains several anticancer compounds. Mutant plants have a higher cytotoxic effect than wild-type plants. This study aimed to devise ARMS PCR SNPs differentiate T. mutants A modeling was also...

Cancer is a large group of diseases that occur in almost any organ or tissue the body when abnormal cells get out normal and invade adjacent cells. In women especially, breast cancer among most common types cancer. These will form tumor can be seen on an x-ray. Some traditional treatments have been carried out, including using local herbal plants such as rodent tubers ( Typhonium flagelliforme ). Rodent tuber contains stigmasterol which has high anticancer effect was found several mutant...

Abstract Typhonium flagelliforme Lodd. is well known as rodent tuber herbal plant has been successfully irradiated by using gamma rays irradiation technique to increase the bioactive compounds. Despite potential of mutant plants, idea came up develop functional food formulations into nanoemulsion systems. Nanoemulsion composed extract, glycerol, DMSO, tween 80, and water in different concentrations. The extract DMSO were formulated with a fixed concentration 0.1% weight formula, emulsifier...

Rodent tuber (Typhonium flagelliforme) is one of the Indonesian herbs that has not yet been developed as an anticancer drug.The local name rodent plant produced superior mutant strains with higher levels and bioactive compounds.Compared to mother plant, GCMS analysis detected four new compounds.A ethanol extract was tested in vitro against MCF7 (breast) cancer cells.In previous studies, extracts from different varieties showed cytotoxic effects on cells at much lower IC50 doses up 10fold...

Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Twitter Facebook Reddit LinkedIn Tools Reprints and Permissions Cite Search Site Citation Nesti Fronika Sianipar, Reflinur, Muhammad Dzulkifly Ashan, Khoirunnisa Assidqi, Dwityantari Widyaningrum, Ragapadmi Purnamaningsih; Direct verification of bogor superior mutant (Typhonium flagelliforme) using RAPD marker techniques sequencing analysis. AIP Conf. Proc. 25 January 2023; 2554 (1): 090002....