A5046902743- Neurobiology and Insect Physiology Research
- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Axon Guidance and Neuronal Signaling
- Plant Molecular Biology Research
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Genetics, Aging, and Longevity in Model Organisms
- RNA modifications and cancer
- RNA Research and Splicing
- Physiological and biochemical adaptations
- Cellular transport and secretion
- Advanced Fluorescence Microscopy Techniques
- Genomics and Chromatin Dynamics
- Animal Behavior and Reproduction
- RNA and protein synthesis mechanisms
- Cell Image Analysis Techniques
- Cardiac electrophysiology and arrhythmias
- Mitochondrial Function and Pathology
- Nerve injury and regeneration
- Endoplasmic Reticulum Stress and Disease
- Cell death mechanisms and regulation
- Zebrafish Biomedical Research Applications
- Cholinesterase and Neurodegenerative Diseases
- Insect and Arachnid Ecology and Behavior
The Francis Crick Institute
2020-2022
King's College London
2017-2022
Guy's Hospital
2019
Bernstein Center for Computational Neuroscience Tübingen
2017
University of Tübingen
2017
Alternative splicing increases neuronal transcriptomic complexity throughout animal phylogeny. To delve into the mechanisms controlling assembly and evolution of this regulatory layer, we characterized microexon program in Drosophila compared it with that mammals. In nonvertebrate bilaterians, is restricted to neurons by posttranscriptional processing enhancer microexons (eMIC) domain Srrm234 . , dependent on regulation Elav/Fne. eMIC deficiency or misexpression leads widespread neurological...
Building arborisations of the right size and shape is fundamental for neural network function. Live imaging in vertebrate brains strongly suggests that nascent synapses are critical branch growth during development. The molecular mechanisms underlying this largely unknown. Here we present a novel system Drosophila studying development complex live, vivo metamorphosis. In growing see dynamics localisations presynaptic proteins very similar to ‘synaptotropic growth’ described fish/frogs. These...
Changes to the structure and function of neural networks are thought underlie evolutionary adaptation animal behaviours. Among many developmental phenomena that generate change programmed cell death (PCD) appears play a key role. We show occurs continuously throughout insect neurogenesis happens soon after neurons born. Mimicking an role for increasing numbers, we artificially block PCD in medial neuroblast lineage Drosophila melanogaster , which results production ‘undead’ with complex...
Two-photon (2P) microscopy is a cornerstone technique in neuroscience research. However, combining 2P imaging with spectrally arbitrary light stimulation can be challenging due to crosstalk between and fluorescence detection. To overcome this limitation, we present simple low-cost electronic solution based on an ESP32 microcontroller TLC5947 LED driver rapidly time-interleave detection epochs during scans. Implemented for less than $100, our design independently drive up 24 spectrum LEDs...
Summary Neurons draw on alternative splicing for their increased transcriptomic complexity throughout animal phylogeny. To delve into the mechanisms controlling assembly and evolution of this regulatory layer, we characterized neuronal microexon program in Drosophila compared it with that mammals. We found , is restricted to neurons by post-transcriptional processing enhancer microexons (eMIC) domain Srrm234 Elav Fne. eMIC deficiency or misexpression leads widespread neurological alterations...
ABSTRACT Changes to the structure and function of neural networks are thought underlie evolutionary adaptation animal behaviours. Among many developmental phenomena that generate change programmed cell death appears play a key role. We show occurs continuously throughout insect neurogenesis happens soon after neurons born. Focusing on two dipterans which have lost flight during evolution we reveal reductions in populations interneurons likely caused by increased development. Mimicking an...
Summary The mouse retina contains a single type of horizontal cell, GABAergic interneuron that samples from all cone photoreceptors within reach and modulates their glutamatergic output via parallel feedback mechanisms. Because cells form an electrically-coupled network, they have been implicated in global signal processing, such as large scale contrast enhancement. Recently, it has proposed can also act locally at the level individual photoreceptors. To test this possibility...
Abstract Two-photon (2P) microscopy is a cornerstone technique in neuroscience research. However, combining 2P imaging with spectrally arbitrary light stimulation can be challenging due to crosstalk between and fluorescence detection. To overcome this limitation, we present simple low-cost electronic solution based on an ESP32 microcontroller TLC5947 LED driver rapidly time-interleave detection epochs during scans. Implemented for less than $100, our design independently drive up 24 spectrum...
Abstract Caspases are best known for their role in programmed cell death but have also been found to be important several non-apoptotic phenomena such as fate specification, migration and terminal differentiation. The dynamics of sub-lethal caspase events the molecular mechanisms regulating them still largely unknown. As more tools visualizing manipulating activation vivo become available, greater insights into this biology being made. Using a new sensitive effector probe, called SR4VH, we...
Abstract Building arborisations of the right size and shape is fundamental for neural network function. Live imaging studies in vertebrate brains strongly suggest that nascent synapses are critical branch growth during development axonal dendritic arborisations. The molecular mechanisms underlying such ‘synaptotropic’ events largely unknown. Here we present a novel system Drosophila studying complex live, vivo metamorphosis. In these growing see relationship between punctate localisations...
ABSTRACT In vitro , developing neurons progress through well-defined stages to form an axon and multiple dendrites. vivo are derived from progenitors within a polarised neuroepithelium it is not clear how initiation observed in relates what occurs complex, three-dimensional environment. Here we show that the position of embryonic zebrafish spinal extremely consistent across neuronal sub-types. We investigated mechanisms may regulate positioning found microtubule organising centres located...