A5022618255- Retinal Development and Disorders
- Advanced Fluorescence Microscopy Techniques
- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Retinal Diseases and Treatments
- CCD and CMOS Imaging Sensors
- Advanced Memory and Neural Computing
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurological Disease Mechanisms and Treatments
- Connexins and lens biology
- Glaucoma and retinal disorders
- Neuroscience and Neural Engineering
- Optical Imaging and Spectroscopy Techniques
- Venomous Animal Envenomation and Studies
- Advanced Optical Sensing Technologies
- Calpain Protease Function and Regulation
- Cell Image Analysis Techniques
University of Tübingen
2017-2022
Bernstein Center for Computational Neuroscience Tübingen
2017-2022
Senckenberg Centre for Human Evolution and Palaeoenvironment
2019
In recent years, two-photon calcium imaging has become a standard tool to probe the function of neural circuits and study computations in neuronal populations. However, acquired signal is only an indirect measurement activity due comparatively slow dynamics fluorescent indicators. Different algorithms for estimating spike rates from noisy measurements have been proposed past, but it open question how far performance can be improved. Here, we report results spikefinder challenge, launched...
Abstract Hereditary degeneration of photoreceptors has been linked to over-activation Ca 2+ -permeable channels, excessive -influx, and downstream activation -dependent calpain-type proteases. Unfortunately, after more than 20 years pertinent research, unequivocal evidence proving significant reproducible photoreceptor protection with -channel blockers is still lacking. Here, we show that both D- L-cis enantiomers the anti-hypertensive drug diltiazem were very effective at blocking most...
Current approaches for surveying whole brains neurons activated during a particular state typically rely on immediate early gene (IEG) expression. However, IEG expression is variable across subjects and brain areas, demanding large sample sizes. Further, it cannot determine if the same or different respond to two events. To overcome these issues, we present whole-brain screening method utilizing transgenic mice label at timepoints. An imaging analysis pipeline surveys activity in ~500 areas...
Abstract The retina decomposes visual stimuli into parallel channels that encode different features of the environment. Central to this computation is synaptic processing in a dense layer neuropil, so-called inner plexiform (IPL). Here, types bipolar cells stratifying at distinct depths relay excitatory feedforward drive from photoreceptors amacrine and ganglion cells. Current experimental techniques for studying IPL do not allow imaging entire simultaneously intact tissue. we extend...
Abstract Calcium (Ca 2+ ) dysregulation has been linked to neuronal cell death, including in hereditary retinal degeneration. Ca is thought cause rod and cone photoreceptor death. Spatial temporal heterogeneities disease models have hampered validation of this hypothesis. We examined the role degeneration, assessing activation pattern ‐dependent calpain proteases, generating spatiotemporal maps entire retina cpfl1 mouse model for primary rd1 rd10 used Gaussian process distinguish sequences...
In recent years, two-photon calcium imaging has become a standard tool to probe the function of neural circuits and study computations in neuronal populations. However, acquired signal is only an indirect measurement activity due comparatively slow dynamics fluorescent indicators. Different algorithms for estimating spike trains from noisy measurements have been proposed past, but it open question how far performance can be improved. Here, we report results spikefinder challenge, launched...
Abstract The retina decomposes visual stimuli into parallel channels that encode different features of the environment. Central to this computation is synaptic processing in a dense and thick layer neuropil, so-called inner plexiform (IPL). Here, types bipolar cells stratifying at distinct depths relay excitatory feedforward drive from photoreceptors amacrine ganglion cells. Current experimental techniques for studying IPL do not allow imaging entire simultaneously intact tissue. we extend...
Variability, stochastic or otherwise, is a central feature of neural activity. Yet the means by which estimates variation and uncertainty are derived from noisy observations activity often heuristic, with more weight given to numerical convenience than statistical rigour. For two-photon imaging data, composed fundamentally probabilistic streams photon detections, problem particularly acute. Here, we present pipeline for inference analysis using Gaussian Process regression, applied recordings...
ABSTRACT Hereditary degeneration of photoreceptors has been linked to over-activation Ca 2+ -permeable channels, excessive -influx, and downstream activation -dependent calpain-type proteases. Unfortunately, after more than 20 years pertinent research, unequivocal evidence proving significant reproducible photoreceptor protection with -channel blockers is still lacking. Here, we show that both D- L-cis enantiomers the anti-hypertensive drug diltiazem were very effective at blocking most...
Summary The mouse retina contains a single type of horizontal cell, GABAergic interneuron that samples from all cone photoreceptors within reach and modulates their glutamatergic output via parallel feedback mechanisms. Because cells form an electrically-coupled network, they have been implicated in global signal processing, such as large scale contrast enhancement. Recently, it has proposed can also act locally at the level individual photoreceptors. To test this possibility...
Event Abstract Back to Combining two photon microscopy and CMOS MEA uncover the mechanism of aberrant activity in blind retina Meng-Jung Lee1, 2, 3*, Prerna Srivastava3, 4, 5, Luke Rogerson3, Philipp Berens4, 6, Thomas Euler4, Timm Schubert4, 5 Günther Zeck1 1 Natural Medical Sciences Institute, Neurophysics, Germany 2 Multi Channel Systems MCS GmbH, 3 Centre de formation des diplômés en neuroscience, Universität Tübingen, 4 Werner Reichardt Centrum für Integrative Neurowissenschaften (CIN),...
Abstract Variability, stochastic or otherwise, is a central feature of neural circuits. Yet the means by which variation and uncertainty are derived from noisy observations activity often unprincipled, with too much weight placed on numerical convenience at cost statistical rigour. For two-photon imaging data, composed fundamentally probabilistic streams photon detections, problem particularly acute. Here, we present complete pipeline for inference analysis using Gaussian Process Regression,...
Abstract Calcium (Ca 2+ ) dysregulation has been linked to neuronal cell death, including in hereditary retinal degeneration. Ca is thought cause rod and cone photoreceptor death. Spatial temporal heterogeneities disease models have hampered validation of this hypothesis. We examined the role degeneration, assessing activation pattern -dependent calpain proteases, generating spatio-temporal maps entire retina cpfl1 mouse model for primary rd1 rd10 used Gaussian process distinguish sequences...