A5078918430- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Cancer-related gene regulation
- Insect Resistance and Genetics
- Genomics and Phylogenetic Studies
- Cancer-related molecular mechanisms research
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Genomic variations and chromosomal abnormalities
- MicroRNA in disease regulation
- Insect symbiosis and bacterial influences
- RNA Research and Splicing
- Cardiac Structural Anomalies and Repair
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Ion Channels and Receptors
- Viral Infectious Diseases and Gene Expression in Insects
- Transgenic Plants and Applications
- Research in Cotton Cultivation
- HVDC Systems and Fault Protection
- Bacteriophages and microbial interactions
- Insect and Pesticide Research
- Advanced biosensing and bioanalysis techniques
- Insect-Plant Interactions and Control
Beijing Normal University
2021-2025
Second Hospital of Shandong University
2025
Huazhong Agricultural University
2016-2023
Shandong Academy of Agricultural Sciences
2023
Liuzhou General Hospital
2019-2021
Guangxi Medical University
2019
Shanghai University of Traditional Chinese Medicine
2019
University of Missouri
2011-2015
Gossypium hirsutum is an allotetraploid with a complex genome. Most genes have multiple copies that belong to At and Dt subgenomes. Sequence similarity also very high between gene homologues. To efficiently achieve site/gene-specific mutation quite needed. Due its efficiency robustness, the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 system has exerted broad site-specific genome editing from prokaryotes eukaryotes. In this study, we utilized CRISPR/Cas9 generate...
Summary The CRISPR /Cas9 system has been extensively applied for crop improvement. However, our understanding of Cas9 specificity is very limited in Cas9‐edited plants. To identify on‐ and off‐target mutation an edited crop, we described whole genome sequencing ( WGS ) 14 cotton plants targeted to three genes, negative (Ne) control wild‐type WT In total, 4188–6404 unique single‐nucleotide polymorphisms SNP s) 312–745 insertions/deletions (indels) were detected compared , reference sequences....
Summary The base‐editing technique using CRISPR / nC as9 (Cas9 nickase) or dC (deactivated Cas9) fused with cytidine deaminase is a powerful tool to create point mutations. In this study, novel G. hirsutum ‐Base Editor 3 (Gh BE 3) system has been developed single‐base mutations in the allotetraploid genome of cotton ( Gossypium ). A sequence APOBEC ) and uracil glycosylase inhibitor UGI was inserted into our /Cas9 plasmid pRGEB 32‐GhU6.7). Three target sites were chosen for two genes, Gh CLA...
Summary The CRISPR/Cas9 and Cas12a (Cpf1) tools have been used on a large scale for genome editing. A new effector with single nuclease domain, relatively small size, low‐frequency off‐target effects cleavage capability under high temperature has recently established designated CRISPR/Cas12b (C2c1). Cas12b also shown inducibility in mammalian systems. Therefore, this system is potentially valuable editing the genomes of plant species, such as cotton, that are resistant to temperatures. Using...
Abstract Background Base editors (BEs) display diverse applications in a variety of plant species such as Arabidopsis, rice, wheat, maize, soybean, and cotton, where they have been used to mediate precise base pair conversions without the collateral generation undesirable double-stranded breaks (DSB). Studies single-nucleotide polymorphisms (SNPs) underpinning traits are still challenging, particularly polyploidy SNPs present multiple copies, simultaneous modification all alleles would be...
Abstract Insects pose significant challenges in cotton‐producing regions. Here, they describe a high‐throughput CRISPR/Cas9‐mediated large‐scale mutagenesis library targeting endogenous insect‐resistance‐related genes cotton. This targeted 502 previously identified using 968 sgRNAs, generated ≈2000 T0 plants and achieved 97.29% genome editing with efficient heredity, reaching upto 84.78%. Several potential resistance‐related mutants (10% of 200 lines) their that may contribute to...
Cotton (Gossypium hirsutum) is an allotetraploid species and a typical thermophilic crop that can survive grow well under temperatures up to 45 °C. CRISPR/LbCpf1 (LbCas12a) temperature-sensitive system for plant genome editing (Malzahn et al., 2019) has been successfully applied in such as rice, soya bean, tobacco, maize cotton (Lee 2019; Li 2018; Tang 2017; Xu 2019). However, the temperature sensitivity of LbCpf1 not tested yet, high temperature-resistant crop. In order improve efficiency...
Dosage compensation, the equalized X chromosome gene expression between males and females in Drosophila, has also been found triple metafemales. Inverse dosage effects, produced by genomic imbalance, are believed to account for this modulated expression, but they have not studied on a global level. Here, we show comparison of metafemales (XXX; AA) with normal (XX; high-throughput RNA-sequencing. We that majority X-linked genes exhibit compensation an level similar diploid females. In...
Significance A popular hypothesis to explain dosage compensation of the X chromosome in male Drosophila is that a histone acetylase brought by MSL complex and increases H4 lysine16 acetylation, which mediates increased expression. We investigated properties with series specific gene-targeting global gene-expression experiments. The data indicate does not mediate directly, but rather, its activity overrides high level acetylation counteracts potential overexpression X-linked genes achieve...
Significance This study demonstrates a global impact of genomic imbalance on gene expression and the generality inverse dosage effect aneuploid compensation. The indicates that sex chromosomes genes with sex-biased evolve distinctly in response to dosage-sensitive regulation.
Genes encoding reporter proteins are used as visual marker-assisted tools in genetic transformation well plant breeding. In this study, the red fluorescent protein identified Discosoma sp. coral (DsRed2) was successfully a marker for cotton engineering. DsRed2 expressed two cultivars, JIN668 and YZ1, driven by CaMV-35S promoter via Agrobacterium-mediated transformation. Our results suggest that expression provides an early-stage selection tool transgenic calli observation. Red fluorescence...
N 6 -methyladenosine (m A) in eukaryotic RNA is an epigenetic modification that critical for metabolism, gene expression regulation, and the development of organisms. Aberrant m A components appears a variety human diseases. Drosophila has proven to be involved sex determination regulated by Sxl may affect X chromosome through MSL complex. The dosage-related effects under condition genomic imbalance (i.e., aneuploidy) are related various regulatory mechanisms. Here, we investigated roles...
N 6 -methyladenosine (m A) in eukaryotic RNA is an epigenetic modification that critical for metabolism, gene expression regulation, and the development of organisms. Aberrant m A components appears a variety human diseases. Drosophila has proven to be involved sex determination regulated by Sxl may affect X chromosome through MSL complex. The dosage-related effects under condition genomic imbalance (i.e. aneuploidy) are related various regulatory mechanisms. Here, we investigated roles...
Abstract The inverse dosage effect caused by chromosome number variations shows global consequences in genomic imbalance including sexual dimorphism and an X chromosome-specific response. To investigate the relationship of MSL complex to imbalance, we over-expressed MSL2 autosomal sex chromosomal aneuploids, analyzed different transcriptomes. Some candidate genes involved regulatory mechanisms have also been tested during embryogenesis using TSA-FISH. Here show that de novo assembled on...
Cotton (Gossypium hirsutum) is an important fiber crop worldwide. Insect attack causes cotton yield and quality losses. However, little known about the mechanism of response to insect attack. We simulated feeding by applying oral secretions (OS) wounds, combined transcriptome metabolome analysis investigate how OS from two major pest species (Helicoverpa armigera Spodoptera litura) affect defense responses. found that respectively 12,668 13,379 genes were differentially expressed in...
A promoter of the PNZIP (Pharbitis nil leucine zipper) gene (1.459 kb) was cloned from Pharbitis and fused to GUS (β-glucuronidase) Bacillus thuringiensis endotoxin (Cry9C) genes. Several transgenic PNZIP::GUS PNZIP::Cry9C cotton lines were developed by Agrobacterium-mediated transformation. Strong staining detected in green tissues plants. In contrast, reproductive structures such as petals, anther, immature seeds very faint. Two one cauliflower mosaic virus (CaMV) 35S::Cry9C line selected...
Aneuploidy can globally affect the expression of whole genome, which is detrimental to organisms. Dosage-sensitive regulators usually have multiple intermolecular interactions, and changes in their stoichiometry are responsible for dysregulation regulatory network. Currently, studies on noncoding genes aneuploidy relatively rare. We studied characteristics profiles long RNAs (lncRNAs) transposable elements (TEs) aneuploid Drosophila. It found that lncRNAs TEs affected by genomic imbalance...
Genomic imbalance in aneuploidy is often detrimental to organisms. To gain insight into the molecular basis of aneuploidies humans, we analyzed transcriptome data from several autosomal and sex chromosome aneuploidies. The results showed that human aneuploid cells, genes located on unvaried chromosomes are inversely or proportionally trans-modulated, while a subset varied compensated. Less genome-wide modulation found for compared with due X inactivation retention dosage sensitive regulators...
Aneuploidy, which disrupts the genetic balance due to partial genome dosage changes, is usually more detrimental than euploidy variation. To investigate modulation of gene expression in aneuploidy, we analyzed transcriptome sequencing data autosomal and sex chromosome trisomy Drosophila. The results showed that most genes on varied (cis) present compensation, while remainder (trans) produce widespread inverse effects. Some altered functions pathways were identified as common characteristics...
For patients with coronary heart disease, reperfusion treatment strategies are often complicated by ischemia/reperfusion (I/R) injury (IRI), leading to serious organ damage and malfunction. The miR-21/programmed cell death protein 4 (PDCD4) pathway is involved in the IRI of cardiomyocytes; however, aberrant miR-21 expression remains unexplained. Therefore, this study aimed explore whether circRNA_0031672 downregulates miR-21-5p during I/R determine miR-21-5p-expressing bone marrow...
RNA-Seq techniques generate hundreds of millions short RNA reads using next-generation sequencing (NGS). These can be mapped to reference genomes investigate changes gene expression but improved procedures for mining large datasets extract valuable biological knowledge are needed. RNAMiner—a multi-level bioinformatics protocol and pipeline—has been developed such datasets. It includes five steps: Mapping a genome, calculating values, identifying differentially expressed genes, predicting...