A5027509318- Protease and Inhibitor Mechanisms
- Cell death mechanisms and regulation
- Cell Adhesion Molecules Research
- Cellular transport and secretion
- Bone Metabolism and Diseases
- Caveolin-1 and cellular processes
- Calpain Protease Function and Regulation
- Cancer, Lipids, and Metabolism
- Genetic factors in colorectal cancer
- Higher Education Practises and Engagement
- Innovative Teaching Methods
- Prostate Cancer Treatment and Research
- Biomedical and Engineering Education
- Blood Coagulation and Thrombosis Mechanisms
- Ethics in Clinical Research
- Career Development and Diversity
- Angiogenesis and VEGF in Cancer
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- Peptidase Inhibition and Analysis
- Cancer Cells and Metastasis
- Chemokine receptors and signaling
- Bladder and Urothelial Cancer Treatments
- Evaluation of Teaching Practices
- Experimental Learning in Engineering
University of Windsor
2015-2024
Western University
2023-2024
Windsor Clinical Research
2022-2024
WE-SPARK Health Institute
2022-2024
The Barbara Ann Karmanos Cancer Institute
2005-2023
Wayne State University
2005-2023
Windsor Dermatology
2011
National Institute of Dental and Craniofacial Research
2008
National Institutes of Health
2008
Abstract Background Inflammatory breast cancer (IBC) is the most aggressive form of cancer. In non-IBC, cysteine protease cathepsin B (CTSB) known to be involved in progression and invasion; however, very little about its role IBC. Methods this study, we enrolled 23 IBC 27 non-IBC patients. All patient tissues used for analysis were from untreated Using immunohistochemistry immunoblotting, assessed levels expression CTSB versus tissues. Previously, found that localized caveolar membrane...
Cathepsin B and pro-urokinase plasminogen activator (pro-uPA) localize to the caveolae of HCT 116 human colorectal carcinoma cells, an association mediated by active K-RAS. In this study, we established a stable cell line with gene encoding antisense caveolin-1 (AS-cav-1) examine effects caveolin-1, main structural protein caveolae, on expression localization cathepsin pro-uPA, their cell-surface receptors p11 uPA receptor (uPAR), respectively. AS-cav-1 cells secreted less procathepsin than...
Podosomes mediate cell migration and invasion by coordinating the reorganization of actin cytoskeleton focal matrix degradation. MMP serine proteases have been found to function at podosomes. The lysosomal cysteine cathepsins, a third major class matrix-degrading enzymes involved in tumor tissue remodeling, yet be linked podosomes with exception cathepsin K osteoclasts. Using inhibitors shRNA-mediated depletion, we show that B participates podosomes-mediated degradation v-Src-transformed...
Inflammatory breast cancer (IBC) is an aggressive, metastatic and highly angiogenic form of locally advanced with a relatively poor three-year survival rate. Breast invasion has been linked to proteolytic activity at the tumor cell surface. Here we explored role for active cathepsin B on surface in invasiveness IBC.We examined expression cysteine protease serine urokinase plasminogen activator (uPA), its receptor uPAR caveolin-1 two IBC lines: SUM149 SUM190. We utilized live proteolysis...
Prostate cancer is one of the most common types among Canadian men. Next-generation sequencing using RNA-Seq provides large amounts data that may reveal novel and informative biomarkers. We introduce a method uses machine learning techniques to identify transcripts correlate with prostate development progression. have isolated potential serve as prognostic indicators tremendous value in guiding treatment decisions. Analysis normal versus malignant sets indicates differential expression genes...
Cathepsin B protein and activity are known to localize the basal plasma membrane of colon carcinoma cells following appearance K-ras mutations. Using immunofluorescence subcellular fractionation techniques two human cell lines—one with a mutated allele (HCT 116) daughter line in which has been disrupted (HKh-2)—we demonstrate that localization cathepsin caveolae on surface these is regulated by mutant K-ras. In HCT 116 cells, greater percentage was distributed caveolae, secretion...
In the tumor microenvironment, monocytes respond to paracrine stimuli from breast cancer cells by secreting molecules that participate in growth, invasion, intravasation and metastasis. Here we examined effects of media conditioned MDA-MB-231 human carcinoma (231-CM) on expression secretion proteases cytokines U937 monocytes. We found 231-CM increased U937: 1) proliferation; 2) expression, activity cysteine protease cathepsin B (CTSB); 3) matrix metalloproteinases (MMP)-2 -9; 4)...
Kallikreins are secreted proteases that may play a functional role and/or serve as serum biomarker for the presence or progression of certain types cancers. Kallikrein 6 (KLK6) has been shown to be upregulated in several cancers, including colon. The aims this study were elucidate pathways influence KLK6 gene expression and protein secretion HCT116 human colon cancer cells. Our data indicate central caveolin-1 (CAV-1), main structural caveolae, both secretion. Sucrose gradient subcellular...
(1) Background:One of the most common cancers that affect North American men and worldwide is prostate cancer. The Gleason score a pathological grading system to examine potential aggressiveness disease in tissue. Advancements computing next-generation sequencing technology now allow us study genomic profiles patients association with their different scores more accurately effectively. (2) Methods: In this study, we used novel machine learning method analyse gene expression tumours scores,...
The ability of tumor cells to adhere to, migrate on, and remodel extracellular matrices is mediated by cell surface receptors such as β 1 -integrins. Here we conducted functional live-cell imaging in real time investigate the effects modulating -integrin expression function on proteolytic remodeling matrix. Human breast prostate cancer were grown reconstituted basement membrane containing a quenched fluorescent form collagen IV. Generation cleavage products resulting increases fluorescence...
The breast tumor microenvironment regulates progression of ductal carcinoma in situ (DCIS) to invasive (IDC). However, it is unclear how interactions between epithelial and stromal cells can drive this whether there are reliable microenvironmental biomarkers predict transition DCIS IDC. We used xenograft mouse models a 3D pathomimetic model termed mammary architecture engineering (MAME) study the interplay human myoepithelial (MEPs) cancer-associated fibroblasts (CAFs) on progression. Our...
Thrombin activatable fibrinolysis inhibitor (TAFI) is a plasma zymogen, which can be converted to activated TAFI (TAFIa) through proteolytic cleavage by thrombin, plasmin, and most effectively thrombin in complex with the endothelial cofactor thrombomodulin (TM). TAFIa carboxypeptidase that cleaves carboxyl terminal lysine arginine residues from protein peptide substrates, including plasminogen-binding sites on cell surface receptors. Carboxyl play pivotal role enhancing plasminogen...
Our in vitro studies support a functional link between the induction of cathepsin B gene expression and catabolic restructuring associated with myotube formation during myogenesis vivo. We have tested two predictions that are basic to this hypothesis: (1) active is localized plasma membrane caveolae fusing myoblasts; (2) secreted from myoblasts at physiological pH. During differentiation, L6 rat demonstrated fusion-related increase activity 25/26-kDa, fully processed, form B....
Abstract Inflammatory breast cancer (IBC) is the most aggressive form of cancer, and here, we examined in vitro interactions between human IBC cell line SUM149 U937 naive monocytes. We found an altered morphology, enhanced invasiveness proteolytic activity cells when cultured with or U937-conditioned media (U937-CM). Increases expression cysteine protease cathepsin B caveolin-1 were also detected grown U937-CM, thus suggesting a contribution these proteins to augmented invasion through...
Background: Prostate cancer is complicated by a high level of unexplained variability in the aggressiveness newly diagnosed disease. Given that this one most prevalent cancers worldwide, finding biomarkers to effectively stratify risk patient populations vital next step improving survival rates and quality life after treatment. Materials Methods: In study, we selected dataset consisting 106 prostate samples, which represent various stages developed RNA-Seq technology. Our objective identify...
The objective of this study was to characterize commercially-available cotton fabrics determine their suitability as materials for construction cloth masks personal and public use reduce infectious disease spread. focused on cottons because widespread availability, moderate performance they are recommended inclusion in home-made by international health authorities. Fifty-two were analyzed electron microscopy fabric characteristics weights. Sixteen selected test breathability construct 2-ply...
There has been a growing discourse within higher education to engage with students as partners and harness the strength of faculty working together. Engaging in student-faculty partnerships activities offers benefits both faculty, yet there is less research on these practices science, technology, engineering mathematics (STEM). The purpose this study was conduct Faculty-wide investigation identify challenges engaging Faculty Science at mid-sized university Ontario, Canada. Using mixed-method...