A5071277065- Mass Spectrometry Techniques and Applications
- Ion-surface interactions and analysis
- Microfluidic and Capillary Electrophoresis Applications
- X-ray Spectroscopy and Fluorescence Analysis
- Nuclear Physics and Applications
- Enzyme Structure and Function
- Advanced X-ray Imaging Techniques
- Analytical Chemistry and Chromatography
Universität Greifswald
2021-2023
Gas-phase activation and dissociation studies of biomolecules, proteins their non-covalent complexes using X-rays hold great promise for revealing new insights into the structure function biological samples. This is due to unique properties X-ray molecular interactions, such as site-specific rapid ionization. In this perspective, we report discuss first proof-of-principle X-ray-induced native samples ranging from small 17 kDa monomeric up large 808 protein assemblies conducted at a...
Gas-phase activation and dissociation studies of biomolecules, proteins their non-covalent complexes using X-rays hold great promise for revealing new insights into the structure function biological samples. This...
MS SPIDOC is a novel sample delivery system designed for single (isolated) particle imaging at X-ray Free-Electron Lasers that adaptable towards most large-scale facility beamlines. Biological samples can range from small proteins to MDa particles. Following nano-electrospray ionization, ionic be m/z-filtered and structurally separated before being oriented the interaction zone. Here, we present simulation package developed alongside this prototype. The first part describes how front-to-end...
Ion trajectories have been simulated for an assembly of a linear quadrupole ion-filter and Paul trap with additional pin electrodes MS SPIDOC, project in preparation the study biomolecules by single-particle imaging X-ray pulses. The ion-optical components are based on digital RF guiding trapping fields. In order to carefully handle over wide mass-over-charge range, module presented consists separate filtering accumulation/trapping select ions interest convert beam from continuous ion source...
Abstract The combination of a linear quadrupole ion-filter and Paul trap operated with rectangular guiding field for the filtering accumulation ions within M ass S pectrometry ingle P article I maging D ipole O riented protein C omplexes (MS SPIDOC) prototype [T. Kierspel et al., Anal. Bioanal. Chem. , published online] is characterized. Using cationic caesium-iodide clusters, ion-separation performance, ion accumulation, cooling, ejection via in-trap pin electrodes evaluated. Furthermore,...