Janine-Denise Kopicki
A5070098704- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Computational Drug Discovery Methods
- SARS-CoV-2 and COVID-19 Research
- Mass Spectrometry Techniques and Applications
- Enzyme Structure and Function
- Advanced Proteomics Techniques and Applications
- Synthesis and biological activity
- Immune Cell Function and Interaction
- Respiratory viral infections research
- Viral Infections and Immunology Research
- Viral Infections and Outbreaks Research
- Mosquito-borne diseases and control
- RNA and protein synthesis mechanisms
- Viral Infections and Vectors
- Bacteriophages and microbial interactions
- Animal Disease Management and Epidemiology
Leibniz Institute of Virology (LIV)
2018-2022
Centre for Structural Systems Biology
2021-2022
The coronavirus disease (COVID-19) caused by SARS-CoV-2 is creating tremendous human suffering. To date, no effective drug available to directly treat the disease. In a search for against COVID-19, we have performed high-throughput x-ray crystallographic screen of two repurposing libraries main protease (M
Abstract Major Histocompatibility Complex (MHC) class I molecules selectively bind peptides for presentation to cytotoxic T cells. The peptide-free state of these is not well understood. Here, we characterize a disulfide-stabilized version the human molecule HLA-A*02:01 that stable in absence peptide and can readily exchange cognate peptides. We present X-ray crystal structures HLA-A*02:01, together with have dipeptides bound A F pockets. These structural snapshots reveal amino acid side...
Gas-phase activation and dissociation studies of biomolecules, proteins their non-covalent complexes using X-rays hold great promise for revealing new insights into the structure function biological samples. This is due to unique properties X-ray molecular interactions, such as site-specific rapid ionization. In this perspective, we report discuss first proof-of-principle X-ray-induced native samples ranging from small 17 kDa monomeric up large 808 protein assemblies conducted at a...
Gas-phase activation and dissociation studies of biomolecules, proteins their non-covalent complexes using X-rays hold great promise for revealing new insights into the structure function biological samples. This...
At the plasma membrane of mammalian cells, major histocompatibility complex class I molecules (MHC-I) present antigenic peptides to cytotoxic T cells. Following loss peptide and light chain beta-2 microglobulin (β2m, encoded by B2M), resulting free heavy chains (FHCs) can associate into homotypic complexes in membrane. Here, we investigate stoichiometry dynamics MHC-I FHCs assemblies combining a micropattern assay with fluorescence recovery after photobleaching (FRAP) single-molecule...
Abstract The coronavirus disease (COVID-19) caused by SARS-CoV-2 is creating tremendous health problems and economical challenges for mankind. To date, no effective drug available to directly treat the prevent virus spreading. In a search against COVID-19, we have performed massive X-ray crystallographic screen of two repurposing libraries main protease (M pro ), which essential replication and, thus, potent target. contrast commonly applied fragment screening experiments with molecules low...
Abstract An essential element of adaptive immunity is selective binding peptide antigens by major histocompatibility complex (MHC) class I proteins and their presentation to cytotoxic T lymphocytes. Using native mass spectrometry, we analyze the peptides an empty disulfide-stabilized HLA-A*02:01 molecule and, due its unique stability, determine affinities complexes loaded with truncated or charge-reduced peptides. We find that two anchor positions can be stabilized independently, further...
Abstract At the plasma membrane of mammalian cells, major histocompatibility complex class I molecules (MHC-I) present antigenic peptides to cytotoxic T cells. Following loss peptide and light chain beta-2 microglobulin (β 2 m), resulting free heavy chains (FHCs) can associate into homotypic complexes in membrane. Here, we investigate stoichiometry dynamics MHC-I FHCs assemblies by combining a micropattern assay with fluorescence recovery after photobleaching (FRAP) single molecule...
ABSTRACT An essential element of adaptive immunity is the selective binding peptide antigens by major histocompatibility complex (MHC) class I proteins and their presentation to cytotoxic T lymphocytes on cell surface. Using native mass spectrometry, we here analyze peptides an empty disulfide-stabilized HLA-A*02:01 molecule. This novel approach allows us examine properties diverse peptides. The unique stability our MHC even enables determine affinity complexes, which are suboptimally loaded...
Abstract Major histocompatibility complex class I molecules (MHC I) that lose the antigenic peptide and light chain beta-2 microglobulin (β2m) to become free heavy chains at cell surface are known associate, forming oligomers on plasma membrane insufficiently understood. Here, we investigate homotypic interaction of MHC by combining a printed antibody micropattern assay with fluorescence recovery after photobleaching (FRAP) single molecule co-tracking in order elucidate their molecular...