Preeclampsia is characterized by an imbalance in angiogenic factors, including sEng (soluble endoglin). However, the relationship of with severity preeclampsia, clinical, and laboratory parameters, occurrence adverse outcomes are not fully elucidated. We studied 1002 women preeclampsia. Serum concentrations were measured ELISA. levels significantly different ( P <0.001) patients preeclampsia than healthy pregnancy. In addition, these factors markedly hemolysis, elevated liver enzymes, low...

Fourteen mutations (13 missense and 1 truncation) of the GnRH receptor (GnRHR) have been identified in patients with hypogonadotropic hypogonadism (HH). We previously shown that five can be rescued a peptidomimetic antagonist acts as folding template, stabilizing (otherwise) misfolded GnRHR mutants thereby restoring function. now report this approach rescues 11 13 these mutants. These data indicate surprisingly general nature approach, an observation presents therapeutic opportunities for HH...

The protein:creatinine ratio in random, untimed urine samples correlates with 24-h protein excretion pregnant women and without hypertension. Nevertheless, whether this is appropriate as a screening test for proteinuria still unclear, part because of the paucity large studies.We measured ratios random contents cross-sectional study 927 hospitalized at >/=20-weeks gestational age 2nd cohort 161 women. In group, specimens were obtained before after completion collections, avoiding 1st-morning...

Abstract Context: It has been proposed that preeclampsia may result from of an imbalance in angiogenic factors. Although prolactin (PRL) is mainly related to lactation, it also involved other biological functions, including angiogenesis. Objective: Our objective was determine the relationship among preeclampsia, serum and urinary PRL (uPRL) levels, excretion antiangiogenic fragments urine. Study design: Using a cross-sectional design, uPRL presence isoforms were determined 546 pregnant...

Preeclampsia is characterized by an imbalance in angiogenic factors. Urinary prolactin (PRL) levels and its antiangiogenic PRL fragments have been associated with disease severity. In this study, we assessed whether these biomarkers are increased risk of adverse maternal perinatal outcomes preeclamptic women. We studied 501 women preeclampsia attended at a tertiary care hospital. Serum concentrations soluble fms-like tyrosine kinase-1 (sFlt-1), placental growth factor (PlGF), endoglin...

Preeclampsia is characterized by an imbalance in angiogenic factors such as soluble fms-like tyrosine kinase-1 (sFlt-1) and placental growth factor (PlGF). We herein assessed whether these measured a newly developed automated electrochemiluminescence immunoassay are associated with risk to develop preeclampsia.We performed nested case-control study within cohort of 230 women singleton pregnancies. The included all 37 who eventually preeclampsia 29 normotensive controls. Serum samples were...

Gestational hypertension (GH) and preeclampsia (PE) are characterized by an imbalance in angiogenic factors. However, the relationship among these factors with severity of hypertensive disorders pregnancy (HDP) adverse outcomes not fully elucidated. We examined whether biomarkers related HDP outcomes.Using a cross-sectional design, serum concentrations placental growth factor (PlGF), soluble fms-like tyrosine kinase-1 (sFlt-1), endoglin were determined 764 pregnant women: 75 healthy...

Preeclampsia is characterized by angiogenic imbalance (AI), sFlt-1 (soluble fms-like tyrosine kinase-1)/PlGF (placental growth factor) useful for its diagnosis and prediction of adverse outcomes, but the relationship among degrees AI as assessed this ratio with correct diagnosis, clinical characteristics, outcomes in women preeclampsia are unclear. We studied 810 preeclampsia. Patients were divided into 3 groups based on their degree AI, evaluated sFlt-1/PlGF ratio: no (≤38), mild (>38-<85),...

Every year, approximately 140 million births occur, with the majority being spontaneous deliveries in women without risk factors, resulting vaginal of healthy newborns. To develop a prognostic score to predict probability cesarean section pregnant at term single live fetus presenting initial labor hospital admission. Through case-control study, admission were included. Cases that culminated an emergency and controls who had normal delivery. Clinical history was questioned, complete physical...

Loss of function by 11 13 naturally occurring mutations in the human GnRH receptor (hGnRHR) was thought to result from impaired ligand binding or effector coupling, but actually results misrouting. Homo- heterodimerization mutant receptors with wild-type (WT) occurs for other G protein-coupled and may dominant-negative -positive effects on WT receptor. We tested hypothesis that hGnRHR affected misfolded mutants. mutants were found inhibit GnRHR (measured activation binding). Inhibition...

Objective. To determine in patients with systemic lupus erythematosus (SLE) the relationships among serum total and free prolactin (PRL) levels, isoforms of PRL activity. Methods. In a cross-sectional study, 259 SLE were tested for PRL, fractions obtained after gel filtration chromatography (in 70 sera taken at random) by immunoradiometric assay based on disease Results. A significant correlation between direct levels was found without activity (r = 0.475, P<0.001); however, this less so...

We analyzed the function of mutant GnRH receptor (GnRHR) pairs associated with compound heterozygous patients showing complete or partial forms hypogonadotropic hypogonadism. did this to examine potential interactions between misfolded mutants that may influence net and response pharmacological rescue. Nine GnRHR an unreported combination (L314X(stop)/R262Q) were studied. Coexpression each pair in COS-7 cells resulted active predominant effect (Q106R/L266R, A171T/Q106R, T32I/C200Y,...

Objective. To investigate whether angiogenic factors are associated with risk of developing preeclampsia in pregnant women systemic lupus erythematosus (SLE). Methods. We performed a nested case–control study within cohort SLE singleton pregnancies. The included 42 patients who eventually developed and 75 normal Serum samples were collected at 4-week intervals (from weeks 12 to 36). analyzed for soluble fms-like tyrosine kinase-1 (sFlt-1), placental growth factor (PlGF), endoglin (sEng)....

Introduction: Amniotic fluid (AF) interleukin-6 (IL-6) concentration has been associated to preterm delivery and perinatal morbidity mortality in women with labor intact membranes. Nevertheless, the clinical significance of this biomarker intra-amniotic inflammation (IAI) is still unclear due part paucity large studies. Methods: AF IL-6 concentrations were determined 452 consecutive membranes, categorized into 3 groups: 302 without IAI (IL-6 &lt;2.6 ng/mL), 64 mild 2.6–11.2 86 severe ≥11.3...

Objective To evaluate the usefulness of serial determinations asymmetric dimethylarginine ( ADMA ) and homocysteine (Hcy) concentrations during pregnancy to predict pre‐eclampsia, taking into account maternal obesity B vitamin status. Design Longitudinal study. Setting Two obstetric referral hospitals. Sample hundred fifty‐two 411 women invited participate in Methods The made monthly visits from ≤20 weeks gestation until delivery for measurements plasma , Hcy, vitamins 6 12, folic acid,...

&lt;i&gt;Background and Purpose:&lt;/i&gt; Previous studies have demonstrated that a common polymorphism in the gene encoding 5,10-methylenetetrahydrofolate reductase (MTHFR) is associated with an increased risk for stroke. However, this relation remains controversial. Our aim was to investigate possible association between C677T MTHFR idiopathic ischemic stroke young Mexican-Mestizo population. &lt;i&gt;Methods:&lt;/i&gt; One hundred seventy-eight patients &lt;45 years 183 controls were...