Mutations in the PTEN induced putative kinase 1 (PINK1) gene cause an autosomal recessive form of Parkinson disease (PD). So far, no substrates PINK1 have been reported, and mechanism by which mutations lead to neurodegeneration is unknown. Here we report identification TNF receptor-associated protein (TRAP1), a mitochondrial molecular chaperone also known as heat shock 75 (Hsp75), cellular substrate for kinase. binds colocalizes with TRAP1 mitochondria phosphorylates both vitro vivo. We...

Abstract Aquaculture is emerging as the fastest growing food-producing industry in world because of increasing demand for food fish consumption. However, intensive culture has led to outbreaks various bacterial diseases, resulting annual economic losses aquaculture estimated at billions dollars worldwide. Feeding infected with antibiotic-medicated a general practice but antibiotic resistance development pathogen, higher dose requirement effective control, matter public concern. Therefore,...

To access the relative potency of pesticides to control adult mosquitoes, 19 with various modes action were evaluated against Aedes aegypti, Culex quinquefasciatus Say, and Anopheles quadrimaculatus Say. On basis 24-h LD50 values after topical application, only pesticide that had higher activity than permethrin was fipronil, lower for 107-, 4,849-, 2-fold Ae. Cx. An. respectively. Abamectin, imidacloprid, spinosad, diazinon, carbaryl showed slightly (<20-fold). However, bifenazate very low...

Mosquitoes that transmit human diseases are of major importance to the international public health community. Pesticides remain a component integrated programs control these medically important species. However, very few types pesticides currently registered for mosquito control. A high-throughput screening method using first-instar larvae Aedes aegypti was created and evaluated in our laboratory quickly screen large numbers chemicals activity against mosquitoes. LC50 values representative...

Aedes aegypti (L.) (Diptera: Culicidae) is the primary vector of both dengue and yellow fever. Use insecticides one ways to control this medically important insect pest. However, few new have been developed for mosquito in recent years. As a part our effort develop mosquitoes, an inhibitor apoptosis protein 1 gene (AaeIAP1) was targeted development molecular pesticides. Herein, first time, we report that topically applied AaeIAP1 double-stranded RNA products are able kill female adults Ae....

Aedes aegypti (L.) (Diptera: Culicidae) is the primary vector of both dengue and yellow fever. Use insecticides one ways to control this medically important insect pest. However, few new have been developed for mosquito in recent years. As a part our effort develop mosquitoes, an inhibitor apoptosis protein 1 gene (AaeIAP1) was targeted development molecular pesticides. Herein, first time, we report that topically applied AaeIAP1 double-stranded RNA products are able kill female adults Ae....

Abstract An Aedes aegypti larval toxicity bioassay was performed on compounds representing many classes of natural including polyacetylenes, phytosterols, flavonoids, sesquiterpenoids, and triterpenoids. Among these compounds, two eudesmanolides, alantolactone, isoalantolactone showed larvicidal activities against Ae. and, therefore, were chosen for further structure–activity relationship study. In this study, structural modifications both alantolactone in an effort to understand the...

A field strain of Aedes aegypti (L.) was collected from Puerto Rico in October 2008. Based on LD50 values by topical application, the 73-fold resistant to permethrin compared with a susceptible Orlando strain. In presence piperonyl butoxide, resistance Ae. reduced 15-fold, suggesting that cytochrome P450-mediated detoxification is involved permethrin. To determine P450s might play role permethrin, transcriptional levels 164 P450 genes were Of P450s, 33 significantly (P < 0.05) up-regulated,...

α-Mangostin derived from mangosteen was identified as a mosquito sterol carrier protein-2 inhibitor via high throughput insecticide screening.α-Mangostin tested for its larvicidal activity against third instar larvae of six species, and the median lethal concentration values range 0.84 to 2.90 ppm.The residual α-mangostin examined under semifield conditions.The results indicated that photolytic with half-life 53 min in water full sunlight exposure.The effect on activities major...

Aedes aegypti (L.) (Diptera: Culicidae) is the primary vector of both dengue and yellow fever. Use insecticides one ways to control this medically important insect pest. However, few new have been developed for mosquito in recent years. As a part our collaborative effort search mosquitoes, piperidine was used as base compound further optimization. Herein, we report structure-activity relationships 33 piperidines against adult female Ae. aegypti. On basis 24-h LD50 values after topical...

To access the relative potency of pesticides to control adult mosquitoes, 19 with various modes action were evaluated against Aedes aegypti, Culex quinquefasciatus Say, and Anopheles quadrimaculatus Say. On basis 24-h LD50 values after topical application, only pesticide that had higher activity than permethrin was fipronil, lower for 107-, 4,849-, 2-fold Ae. Cx. An. respectively. Abamectin, imidacloprid, spinosad, diazinon, carbaryl showed slightly (<20-fold). However, bifenazate very low...

Temperature is important for mosquito development and physiological response. Several genes of heat shock protein (HSP) families are known to be expressed in mosquitoes may crucial responding stress induced by elevated temperature. Suppression subtractive hybridization (SSH) was used identify target transcripts treatment female Aedes aegypti. Subtraction performed both directions enriching cDNAs differentially between a non-heat control treatment. Heat Ae. aegypti carried out 1 h at 42°C....