A5027367327- Celiac Disease Research and Management
- Galectins and Cancer Biology
- Mitochondrial Function and Pathology
- Monoclonal and Polyclonal Antibodies Research
- Genetic Neurodegenerative Diseases
- SARS-CoV-2 and COVID-19 Research
- Microbial Metabolites in Food Biotechnology
- Metalloenzymes and iron-sulfur proteins
- Lipid Membrane Structure and Behavior
- Protein Structure and Dynamics
- Genetics and Neurodevelopmental Disorders
- Folate and B Vitamins Research
- Supramolecular Self-Assembly in Materials
- RNA Research and Splicing
- vaccines and immunoinformatics approaches
- Prion Diseases and Protein Misfolding
- Ubiquitin and proteasome pathways
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- interferon and immune responses
- Microscopic Colitis
- Transgenic Plants and Applications
- Immune responses and vaccinations
- Protein Hydrolysis and Bioactive Peptides
- Health Policy Implementation Science
Bielefeld University
2018-2024
University of Buenos Aires
2017-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2013-2024
Fundación Ciencias Exactas y Naturales
2019-2024
Ruhr University Bochum
2021-2024
Instituto de Química y Fisicoquímica Biológicas
2017-2019
Faculty (United Kingdom)
2019
Instituto de Física del Sur
2016
Universidad Nacional del Sur
2015-2016
Centro Científico Tecnológico - Bahía Blanca
2013-2016
Abstract The yeast Pichia pastoris is a cost-effective and easily scalable system for recombinant protein production. In this work we compared the conformation of receptor binding domain (RBD) from severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) Spike expressed in P. well established HEK-293T mammalian cell system. RBD obtained both cells was properly folded, as indicated by UV-absorption, circular dichroism tryptophan fluorescence. They also had similar stability,...
Celiac disease (CD) is a chronic enteropathy elicited by Th1 response to gluten peptides in the small intestine of genetically susceptible individuals. However, it remains unclear what drives induction inflammatory responses this kind against harmless antigens food. In recent work, we have shown that p31-43 peptide (p31-43) from -gliadin can induce an innate immune and may initiate pathological adaptive immunity. The receptors mechanisms responsible for immunity are unknown here present...
Abstract Mitochondria are increasingly recognized as cellular hubs to orchestrate signaling pathways that regulate metabolism, redox homeostasis, and cell fate decisions. Recent research revealed a role of mitochondria also in innate immune signaling; however, the mechanisms how affect signal transduction poorly understood. Here, we show NF‐κB pathway activated by TNF employs platform for amplification shuttling nucleus. treatment induces recruitment HOIP, catalytic component linear...
Gliadin, a protein present in wheat, rye, and barley, undergoes incomplete enzymatic degradation during digestion, producing an immunogenic 33-mer peptide, LQLQPF(PQPQLPY)3 PQPQPF. The special features of that provoke break its tolerance leading to gliadin sensitivity celiac disease remains elusive. Herein, it is reported peptide was not only able fold into polyproline II secondary structure but also depending on concentration resulted conformational transition self-assembly under aqueous...
The 33-mer gliadin peptide, is a highly immunogenic peptide involved in celiac disease and probably other immune pathologies associated to gliadin. spontaneous self-assembly of water reported, providing better insight into oligomers morphology secondary structure.
The receptor binding domain (RBD) of the Spike protein from SARS-CoV-2 is a promising candidate to develop effective COVID-19 vaccines since it can induce potent neutralizing antibodies. We have previously reported highly efficient production RBD in Pichia pastoris, which structurally similar same produced mammalian HEK-293T cells. In this work we designed an multimer with purpose increasing its immunogenicity. multimeric particles by transpeptidation reaction between expressed P. pastoris...
Celiac disease (CeD) is an autoimmune disorder triggered by gluten proteins, affecting approximately 1 % of the global population. The 33-mer deamidated gliadin peptide (DGP) a metabolically modified wheat-gluten superantigen for CeD. Here, we demonstrate that DGP spontaneously assembles into oligomers with diameter 24 nm. present two main secondary structural motifs-a major polyproline II helix and minor β-sheet structure. Importantly, in presence oligomers, there statistically significant...
Celiac disease (CeD) is a highly prevalent chronic immune-mediated enteropathy developed in genetically predisposed individuals after ingestion of group wheat proteins (called gliadins and glutenins). The 13mer α-gliadin peptide, p31-43, induces proinflammatory responses, observed by vitro assays animal models, that may contribute to innate immune mechanisms CeD pathogenesis. Since cellular receptor for p31-43 has not been identified, this raises the question whether peptide could mediate...
Iron–sulfur clusters are essential cofactors in many biochemical processes. ISD11, one of the subunits protein complex that carries out cluster assembly mitochondria, is necessary for cysteine desulfurase NFS1 stability and function. Several authors have recently provided evidence showing ISD11 interacts with acyl carrier (ACP). We carried coexpression human mitochondrial ACP E. coli. This work shows form a soluble, structured, stable able to bind subunit modulating its activity. Results...
The neurodegenerative disease Friedreich ataxia results from a deficiency of frataxin, mitochondrial protein. Most patients have GAA expansion in the first intron both alleles frataxin gene, whereas minority them are heterozygous for and contain mutation other allele. Frataxin has been claimed to participate iron homeostasis biosynthesis FeS clusters, however its role pathways is not unequivocally defined. In this work we combined different advanced spectroscopic analyses explore...
Abstract The yeast Pichia pastoris is a cost-effective and easily scalable system for recombinant protein production. In this work we compared the conformation of receptor binding domain (RBD) from SARS-CoV-2 Spike expressed in P. well established HEK-293T mammalian cell system. RBD obtained both cells was properly folded, as indicated by UV-absorption, circular dichroism tryptophan fluorescence. They also had similar stability, temperature-induced unfolding (observed T m were 50 °C 52...
In recent years, the mammalian mitochondrial protein complex for iron–sulfur cluster assembly has been focus of important studies. This is partly because its high degree relevance in cell metabolism and mutations involved proteins are cause several human diseases. Cysteine desulfurase NFS1 key enzyme complex. At present, it well-known that active form stabilized by small ISD11. this work, structure ACP-ISD11 heterodimer was determined at 2.0 Å resolution. forms a cooperative unit ionic...
The proteolytic resistant 33-mer gliadin peptide is an immunodominant fragment in gluten and responsible for the celiac disease other gluten-related disorders. Meanwhile, primary structure of associated with adaptive immune response patients, structural transformation into protofilaments activates a primordial innate human macrophages. This means that accumulation, oligomerisation could be unknown first event triggers disease. Herein, we reveal early stepwise mechanism by combining multiple...