A5036041313- Olfactory and Sensory Function Studies
- Neurobiology and Insect Physiology Research
- Erythrocyte Function and Pathophysiology
- Adipose Tissue and Metabolism
- Ion channel regulation and function
- Ion Channels and Receptors
- T-cell and B-cell Immunology
- Spine and Intervertebral Disc Pathology
- Cellular Mechanics and Interactions
- Single-cell and spatial transcriptomics
- Marine Invertebrate Physiology and Ecology
- Thermoregulation and physiological responses
- Hearing, Cochlea, Tinnitus, Genetics
- Pain Mechanisms and Treatments
National Institute of Dental and Craniofacial Research
2017-2025
National Institutes of Health
2017-2021
Somatosensory neurons with cell bodies in the dorsal root ganglia (DRG) project to skin, muscles, bones, and viscera detect touch temperature as well mediate proprioception many types of interoception. In addition, somatosensory system conveys clinically relevant noxious sensations pain itch. Here, we used single nuclear transcriptomics characterize transcriptomic classes human DRG that these diverse stimuli. Notably, multiple have features resemble their mouse counterparts although...
The trigeminal ganglion contains somatosensory neurons that detect a range of thermal, mechanical and chemical cues innervate unique sensory compartments in the head neck including eyes, nose, mouth, meninges vibrissae. We used single-cell sequencing situ hybridization to examine cellular diversity mice, defining thirteen clusters neurons. show are well conserved dorsal root ganglia suggesting they represent distinct functional classes not specialization associated with their targets....
Despite the potential importance of genital mechanosensation for sexual reproduction, little is known about how perineal touch influences mating. We explored affords exquisite awareness genitals and controls reproduction in mice humans. Using genetic strategies vivo functional imaging, we demonstrated that mechanosensitive ion channel PIEZO2 (piezo-type component 2) necessary behavioral sensitivity to touch. function needed triggering a touch-evoked erection reflex successful mating both...
Single cell sequencing has provided unprecedented information about the transcriptomic diversity of somatosensory systems. Here, we describe a simple and versatile in situ hybridization (ISH)-based approach for mapping this back to tissue. We illustrate power by demonstrating that ISH localization with just 8 probes is sufficient distinguish all major classes neurons sections trigeminal ganglion. To further simplify make class assignment segmentation automatic, developed machine learning...
Toothache is a common painful consequence of damage to the teeth, particularly when coupled infection. Clinical restoration tooth integrity, sometimes involving physical and chemical sterilization with nerve fiber ablation (i.e., endodontic therapy), generally alleviates pain allows long-lasting dental function. These observations raise questions regarding biological role tooth-innervating fibers. Here, we determined transcriptomic diversity sensory neurons that can be retrogradely labeled...
Abstract Somatosensory neurons with cell bodies in the dorsal root ganglia (DRG) project to skin, muscles, bones, and viscera detect touch temperature as well mediate proprioception many types of interoception. In addition, somatosensory system conveys clinically relevant noxious sensations pain itch. Here we used single nuclear transcriptomics characterize classes human DRG that these diverse stimuli. Notably, multiple have transcriptomic features resemble their mouse counterparts although...