A5013524162- Cervical Cancer and HPV Research
- SARS-CoV-2 and COVID-19 Research
- Endometrial and Cervical Cancer Treatments
- COVID-19 Clinical Research Studies
- Genital Health and Disease
- COVID-19 epidemiological studies
- Viral-associated cancers and disorders
- Cancer-related molecular mechanisms research
- Long-Term Effects of COVID-19
- Molecular Biology Techniques and Applications
- Global Cancer Incidence and Screening
- Polyomavirus and related diseases
- SARS-CoV-2 detection and testing
- Hepatitis B Virus Studies
- Plant Virus Research Studies
- Colorectal and Anal Carcinomas
- Reproductive tract infections research
- Reproductive System and Pregnancy
- Carcinogens and Genotoxicity Assessment
- Dental materials and restorations
- Plant and Fungal Interactions Research
- Bacteriophages and microbial interactions
- Nonmelanoma Skin Cancer Studies
- COVID-19 and Mental Health
- Indoor Air Quality and Microbial Exposure
Karolinska Institutet
2016-2025
Karolinska University Hospital
2017-2022
Fraunhofer Institute for Toxicology and Experimental Medicine
2016
PURPOSE Detection of human papillomavirus (HPV) by polymerase chain reaction in invasive cervical cancer is strongly associated with prognosis but previous studies have not considered sequencing efforts. We aimed to assess the association when also including comprehensive analysis HPV infection deep and a longer follow-up period. MATERIALS AND METHODS subjected all 392 2,845 cases that were reaction–negative for RNA on NovaSeq 6000 platform (Illumina) identified an additional 169 as...
Background High-risk human papillomavirus (hrHPV) infection is established as the major cause of invasive cervical cancer (ICC). However, whether hrHPV status in tumor associated with subsequent prognosis ICC controversial. We aim to evaluate association between and using national registers comprehensive (HPV) genotyping. Methods findings In this nationwide population-based cohort study, we identified all diagnosed Sweden during years 2002–2011 (4,254 confirmed cases), requested archival...
The Swedish National Cervical Screening Registry collects and evaluates comprehensive, nationwide health data to optimise organised cervical cancer prevention. Since all specimens are saved in biobanks, population-based from the should be available for analysis linkage with other information. We identified cancers diagnosed Sweden during 2002–2011 (4254 confirmed cases) requested tissue blocks retrieve human papillomavirus (HPV) genotype using general primer PCR Luminex genotyping real-time...
Our study used a refined case-control cervical cancer Audit framework to investigate effectiveness of screening, with measures three screening failures: irregular-participation, developed after cytological abnormalities and normal results. The register-based included 4,254 cases diagnosed in Sweden during 2002-2011, 30 population-based controls per case. We conditional logistic regression models examine relative risks relation participation results the past two rounds from 6 months before...
Abstract Background Human papillomavirus (HPV) is a necessary cause of cervical cancer, although some invasive cancers may test negative by HPV PCR. We previously requested all in Sweden during 10 years and subjected them to also optimised methods for deep sequencing formalin-fixed paraffin-embedded samples. Methods Using Novaseq 6000, we simultaneously sequenced total DNA cDNA from 392 PCR-negative cancers. Non-human reads were queried against known HPVs. The complete database now contains...
Abstract Background Most countries in the world have launched human papillomavirus (HPV) vaccination programmes and declining prevalences of HPV are reported. We aimed to disentangle influences calendar time, birth cohort age by analysing population-based cervical screening programme using age-period-cohort modelling. Methods All 836,314 primary HPV-based tests from women aged 23-64 between 2014-2023 capital region Sweden were identified Swedish National Cervical Screening Registry. The odds...
To examine the association of cervical cytology screening with risk adenosquamous cell carcinoma (ASC) and rare histological types invasive (RICC), using comprehensive registry data, to assess tumour human papillomavirus status ASC RICC.Nationwide, population based, nested case-control study.Sweden.All cases in Sweden during 2002-11 (4254 confirmed after clinical histopathological review). 338 were neither squamous nor adenocarcinoma, including 164 174 RICC (glassy carcinoma, clear small...
The elimination of cervical cancer rests on high efficacy human papillomavirus (HPV) vaccines. HPV type distribution among cases invasive (ICC) is used to make predictions about the impact eliminating different types HPV, but accumulating evidence differences in age-specific incidence by exists. We one largest population-based series genotyping ICCs (n = 2,850; Sweden, 2002-2011) estimate ICC and obtain estimates cancer-protective removal types. In base case, had 2 peaks, standardized...
Most cancer forms known to be caused by viruses are increased among the immunosuppressed, but several without established viral etiology also increased, notably nonmelanoma skin carcinoma (NMSC). We followed all 13,429 solid organ transplantation patients in Sweden for occurrence after transplantation. requested these tumor specimens and sequenced first 89 received (62 NMSCs, 27 other cancers). The sequences were analyzed based on two bioinformatics algorithms (paracel-blast (sensitive...
De novo assembly of sequence reads from next generation sequencing platforms is a common strategy for detecting presence and viruses in biospecimens. Amplification artifacts several related the same specimen can lead to erroneous, chimeric sequences. We now report that such chimeras also occur between viral non-viral biological sequences incorrectly joined together which may cause erroneous detection viruses, highlighting importance performing chimera checking step bioinformatics pipelines....
Introduction. Cervical cancer is caused by human papillomavirus (HPV), but some cases may test HPV-negative. We previously tested 2850 Swedish and found that 394/2850 (13.8 %) HPV DNA-negative PCR. Sequencing the most thorough method to assess status. Aim. wished whether deep sequencing might detect sequences among these HPV-negative cervical specimens, increase likelihood of detecting transcriptionally active infections. Methodology. Out cases, we sequenced a random sample 92 PCR-negative...
Close monitoring of vaccination coverage is important for cervical cancer prevention efforts. The study aims to describe the HPV by dose in girls eligible within Sweden's childhood immunization program and provide an estimate on timing compliance. Vaccination records between 2012 March 2019 were obtained born 2000-2006 from registers Sweden. mid-time population counts respective birth cohorts taken as denominator. Full-dose with at least one vaccine calculated two-dose three-dose regimen,...
Abstract The extent that antibodies to SARS-CoV-2 may protect against future virus-associated disease is unknown. We invited all employees (n = 15,300) at work the Karolinska University Hospital, Stockholm, Sweden participate in a study examining SARS-Cov-2 relation registered sick leave. For consenting 12,928 healthy hospital could be determined and compared participant leave records. Subjects with viral serum were not excess risk for (adjusted odds ratio (OR) controlling age sex: 0.85 [95%...
Abstract The Cervical Screening Cohort enrols women screened for human papillomavirus (HPV) and cervical abnormalities within the capital region of Sweden from organised screening program non-organised testing samples. cohort started in 2011 has enrolled more than 670,000 women, contributing with 1.2 million biobanked is systematically updated individual-level data Swedish National Registry (NKCx). Key variables include birthdate, sampling date, cytological, histopathological HPV analysis...
Data are lacking regarding which human papillomavirus (HPV) types cause high-grade cervical neoplasia (CIN2+) in people with HIV Europe. We assessed HPV associated CIN2+ women living Sweden by status.The Swedish National Registry, the Population and Cervical Screening Registry were linked. tissue blocks of 130 (WLWH) 234 HIV-negative women, matched for country birth (1:2), retrieved from bio-banks genotyped. Adjusted ORs (adjOR), stratified birth, calculated using conditional logistic...
Abstract Background Whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) positivity among asymptomatic subjects reflects past or future disease may be difficult to ascertain. Methods We tested 9449 employees at Karolinska University Hospital, Stockholm, Sweden for SARS-CoV-2 RNA and antibodies, linked the results sick leave records, determined associations with using multinomial logistic regression. Results Subjects high amounts of virus, indicated by polymerase chain...
Studies investigating presence of viruses in cancer often analyze case series cancers, resulting detection many that are not etiologically linked to the tumors where they found. The incidence virus-associated cancers is greatly increased immunocompromised individuals. Non-melanoma skin (NMSC) also and a variety have been detected NMSC. As immunosuppressed patients develop multiple independent NMSCs, we reasoned consistently present might be more likely involved tumorigenesis. We sequenced 8...
In Sweden, home care services is a major external contact for older persons.Five service companies in Stockholm, enrolled 405 employees to study including serum IgG SARS-CoV-2 and virus throat swabs.20.1% (81/403) of were seropositive, about twice as many simultaneously reference population (healthcare workers entirely without patient contact, n = 3671; 9.7% seropositivity). 13/379 (3.4%) had current infection (PCR positivity). Amongst these, 5 also seropositive 3 positive with low amounts...
Abstract Background The extent that antibodies to SARS-CoV-2 may protect against future virus-associated disease is unknown. Method We analyzed 12928 healthy hospital employees for and compared results participant sick leave records (Clinical trial registration: https:ClinicalTrials.gov NCT04411576 ). Results Subjects with viral serum were not at excess risk (Odds Ratio (OR): 0.85 (95% Confidence Interval (CI) (0.85 (0.43-1.68)). By contrast, subjects had an in the past weeks (OR: 3.34...
Objective: The aim of this study was to assess failure after treatment high-grade cervical intraepithelial neoplasia (CIN2+) by HIV status and human papillomavirus (HPV) type. Design: A population-based register study. Methods: Swedish National Registry, the Population Registry Cervical Screening were linked identify all women in Stockholm Gothenburg counties (Sweden) living with diagnosed CIN2+ sometime between 1983 2014 ( n = 179). HIV-negative controls CIN2+, matched (2 : 1) for country...
5525 Background: The role of human papillomavirus (HPV) in development from oncogenic infection to invasive cervical cancer (ICC) has been well established. However, the association HPV genotypes and prognosis ICC is controversial. Methods: We identified all diagnosed Sweden during years 2002-2011 (4254 confirmed cases after clinical histo-pathological review), requested archival formalin-fixed, paraffin-embedded blocks subjected them comprehensive genotyping. Twenty out twenty-five archives...
Cervical screening is increasingly switching to human papillomavirus (HPV) testing. In many settings, the switch has involved one or several co-tests (testing using both cytology and HPV) in guidelines, ensure safety. When Sweden switched HPV testing 2015 guidelines included a co-test at age 41. To evaluate effect of co-testing, we identified all 208,701 women resident who 2019 were 40-42 years old thus eligible for co-testing. All cervical samples, results test subsequent biopsies Swedish...