A5080680689- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Force Microscopy Techniques and Applications
- Nanowire Synthesis and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Advanced MEMS and NEMS Technologies
- Cancer Research and Treatments
- RNA and protein synthesis mechanisms
- Advanced Biosensing Techniques and Applications
- Biosensors and Analytical Detection
- Analytical Chemistry and Sensors
- Mechanical and Optical Resonators
- Silicon Nanostructures and Photoluminescence
- Nanofabrication and Lithography Techniques
- Microfluidic and Bio-sensing Technologies
- DNA and Nucleic Acid Chemistry
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- Nanoplatforms for cancer theranostics
- Acoustic Wave Resonator Technologies
- Advanced Proteomics Techniques and Applications
- Immunotherapy and Immune Responses
National Research Council
2008-2025
Istituto di Fisiologia Clinica
2015-2025
Consorzio Roma Ricerche
2017
University of Pisa
2000-2016
Piaggio (Italy)
2000-2003
In-field analysis (e.g., clinical and diagnostics) using nanostructured porous silicon (PSi) for label-free optical biosensing has been hindered so far by insufficient sensitivity of PSi biosensors. Here we report on a interferometric aptasensor able to specifically detect tumor necrosis factor alpha (TNFα, protein biomarker inflammation sepsis) at concentration down 3.0 nM with signal-to-noise ratio (S/N) 10.6 detection limit (DL) 200 pM. This represents 10 000-fold improvement respect...
Aptamers able to bind efficiently cell-surface receptors differentially expressed in tumor and healthy cells are emerging as powerful tools perform targeted anticancer therapy. Here, we present a novel oligonucleotide chimera, composed by an RNA aptamer DNA decoy. Our assembly is (i) target via antitransferrin receptor (ii) selective codelivery of chemotherapeutic drug (Doxorubicin) inhibitor cell-survival factor, the nuclear factor κB decoy oligonucleotide. Both payloads released under...
The study of biological systems for the protection environment, food, and health is among most important research fields in scientific literature [...]
This paper presents the development of a lab-on-chip, based on thin-film sensors, suitable for DNA treatments. In particular, system performs on-chip amplification and separation double-strand into single-strand DNA, combining polydimethylsiloxane microfluidic network, electronic devices, surface chemistry. Both analytical procedures rely integration same glass substrate metal heaters amorphous silicon temperature sensors to achieve uniform distribution (within ±1 °C) in heated area precise...
BRAF represents one of the most frequently mutated protein kinase genes and BRAFV600E mutation may be found in many types cancer, including hairy cell leukemia (HCL), anaplastic thyroid cancer (ATC), colorectal melanoma. Herein, a fluorescent probe, based on structure highly specific inhibitor Vemurafenib (Vem, 1) featuring NIR fluorophore cyanine-5 (Cy5), was straightforwardly synthesized characterized (Vem-L-Cy5, 3), showing promising spectroscopic properties. Biological validation...
The use of chromatography coupled with mass spectrometry (MS) analysis is a powerful approach to identify proteins, owing its capacity fractionate molecules according different chemical features. first protein expression map vascular smooth muscle cells (VSMC) was published in 2001 and since then other papers have been produced. most detailed two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) presented by Mayr et al who identified 235 corresponding the 154 abundant unique proteins...
Vascular restenosis is affecting 30-40% of patients treated by percutaneous coronary angioplasty (PTCA). The advent stenting reduced but not abolished restenosis. introduction drug eluting stent (DES) further restenosis, impaired endothelization exposed to intracoronary thrombosis as late adverse event. It widely accepted that the endothelial denudation and wall damages expose Smooth Muscle Cells (VSMC) multiple growth factors plasma circulating agents thus activating migration proliferative...
As the endothelium still represents ideal surface for cardiovascular devices, different endothelialization strategies have been attempted biocompatibility and nonthrombogenicity enhancement. Since endothelial progenitor cells (EPCs) could accelerate endothelialization, preventing thrombosis restenosis, aim of this study was to use oligonucleotides (ONs) biofunctionalize stents EPC binding. In order optimize functionalization procedure before its application cobalt-chromium (Co/Cr) stents,...
Abstract Ubiquitous in nature, polyamines (PAs) are a class of low-molecular aliphatic amines critically involved cell growth, survival and differentiation. The polycation behavior is validated as successful strategy delivery systems to enhance oligonucleotide loading cellular uptake. In this study, the chemical features functional roles PA spermidine synergistically exploited synthesis bioactive functionalization SiO 2 -based structures. Inspired by biosilicification, role assessed both...
Curcumin is a natural polyphenol that exhibits variety of beneficial effects on health, including anti-inflammatory, antioxidant, and hepato-protective properties. Due to its poor water solubility membrane permeability, in the present study, we prepared characterized water-stable, freely dispersible nanoformulation curcumin. Although potential curcumin nanoformulations hepatic field has been studied, there are no investigations their effect fibrotic pathological conditions involving...
We selectively immobilized organofunctional silanes on top of polycrystalline silicon-germanium (poly-SiGe) layers, as a first step towards the fabrication poly-SiGe-based bioMEMS (biomedical MicroElectroMechanicalSystems) by means standard UV photolithography. 3-aminopropyl-dimethyl-ethoxysilane (APDMES) and 3-aminopropyl-triethoxysilane (APTES) molecules were onto resist-patterned poly-SiGe surfaces. The protocols for surface hydroxylation silane immobilization designed to be...
A new optical platform for the interrogation of a polymethylmethacrylate (PMMA) multichannel array chemical and biochemical parameters is described. It consists plastic chip formed by two pieces PMMA properly shaped in order to obtain different microchannels, 500 mum width 400 height. fluorescent sensing layer immobilized on internal wall microchannel. The emitted light travels along thickness upper piece it detected with an fiber connected spectrum analyzer. anisotropy fluorescence...
Here we report on a high-sensitivity interferometric technique, namely Interferogram Averaged over Wavelength – IAW reflectance spectroscopy, enabling nanomolar detection (both nonspecific and specific) of proteins to be accomplished in direct (without any amplification) label-free mode using porous silicon (PSi) interferometers. The spectroscopy is based both calculation elaboration spectral interferograms. Interferograms are obtained by subtracting (intensity, wavelength wavelength)...
Vascular Smooth Muscle Cells (VSMCs), due to their plasticity and ability shift from a physiological contractile-quiescent phenotype pathological proliferating-activated status, play central role in the onset progression of atherosclerosis cardiovascular diseases. PDGF-BB, among series cytokines growth factors, has been identified as critical factor this phenotypic switch. In order obtain new insights on molecular effects triggered by hammerhead ribozyme targeting membrane receptor PDGFR-β...