A5061277054- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- DNA Repair Mechanisms
- RNA modifications and cancer
- biodegradable polymer synthesis and properties
- Biopolymer Synthesis and Applications
- Protein Structure and Dynamics
- Signaling Pathways in Disease
- Supramolecular Self-Assembly in Materials
- Protease and Inhibitor Mechanisms
- Enzyme Structure and Function
- Trace Elements in Health
- Prion Diseases and Protein Misfolding
- Calpain Protease Function and Regulation
- Hydrogels: synthesis, properties, applications
- Alzheimer's disease research and treatments
- Genetics, Bioinformatics, and Biomedical Research
- Cancer-related molecular mechanisms research
Kyoto University
2013-2023
Kyoto College of Medical Science
2017-2021
Advanced Energy (United States)
2019-2020
Kyoto Bunkyo University
2019
Graduate School USA
2015
University of Warsaw
2012
Ritsumeikan University
2012
The first observation of NMR signals DNA/RNA introduced into living human cells by means pore formation SLO and resealing.
Under favorable conditions of pH and temperature, poly(L-glutamic acid) (PLGA) adopts different types secondary quaternary structures, which include spiral assemblies amyloid-like fibrils. Heating acidified solutions PLGA (or PDGA) triggers formation β(2)-type aggregates with morphological tinctorial properties typical for amyloid In contrast to regular antiparallel β-sheet (β(1)), the amide I' vibrational band β(2)-fibrils is unusually red-shifted below 1600 cm(-1), has been attributed...
The alkylation of the specific higher-order nucleic acid structures is great significance in order to control its function and gene expression. In this report, we have described T-T mismatch selective with a vinyldiaminotriazine (VDAT)-acridine conjugate. selectively proceeded at N3 position thymidine on mismatch. Interestingly, alkylated induced base flipping complementary duplex. model experiment for CTG repeats DNA which causes myotonic dystrophy type 1 (DM1), observed reaction rate one...
The interaction of a macrocyclic tetraoxazole compound, L2H2-4OTD (1), with two aminoalkyl side chains and telomeric i-motif, was investigated by means electrophoretic mobility shift assay, circular dichroism spectroscopy, mass spectrometry NMR spectroscopy analyses. results indicate that 1 interacts the i-motif structure at preferred binding sites.
The formation of parallel and antiparallel triplex structures in living human cells is proven for the first time.
Abstract Base-pair opening is a fundamental property of nucleic acids that plays important roles in biological functions. However, studying the base-pair dynamics inside living cells has remained challenging. Here, to determine kinetics human cells, exchange rate constant ( $${k}_{{{{{{\rm{ex}}}}}}}$$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:msub> <mml:mrow> <mml:mi>k</mml:mi> </mml:mrow> <mml:mi>ex</mml:mi> </mml:msub> </mml:math> ) imino proton with solvent water...
An RNA aptamer that strongly binds to a target molecule has the potential be nucleic acid drug inside living human cells. To investigate and improve this potential, it is critical elucidate structure interaction of aptamers We examined an for HIV-1 Tat (TA), which had been found trap repress its function in first used vitro NMR examine between TA part containing binding site trans-activation response element (TAR). It was revealed two U-A∗U base triples are formed upon Tat. This assumed...
The neurodegenerative disorder Alzheimer's disease (AD) is associated with the accumulation of misfolded proteins. Some recent studies suggested that amyloid beta (Aβ) forms soluble oligomers, protofibrils, and fibrils; Aβ oligomers being more toxic than fibrils. Surprisingly, these reportedly bind to prion protein (PrP), which acts as a receptor on cell membrane, possibly resulting in AD. Thus, it thought compounds can disrupt formation prion‐Aβ oligomer complex may prevent Here, we...
Abstract Translocated in liposarcoma (TLS)/fused sarcoma (FUS) is a multitasking DNA/RNA binding protein implicated cancer and neurodegenerative diseases. Upon DNA damage, TLS recruited to the upstream region of cyclin D1 gene ( CCND1 ) through promotor associated non-coding RNA (pncRNA) that transcribed from tethered at region. Binding pncRNA hypothesized cause conformational change enables its inhibitive interaction with histone acetyltransferases resultant repression expression, although...
The intracellular environment is highly crowded with biomacromolecules such as proteins and nucleic acids. Under conditions, the structural biophysical features of acids have been thought to be different from those in vitro. To obtain high-resolution information on living cells, in-cell NMR method a unique tool. Following first measurement 2009, several interesting insights were obtained using Xenopus laevis oocytes. However, spectrum human cells was not reported until two years ago due...
We introduced an isotopically labeled RNA aptamer for HIV-1 Tat prepared by E. coli transcription into HeLa cells. successfully recorded the first heteronuclear 2D in-cell NMR spectra, which makes it possible to study interaction of with argininamide in living human cells higher resolution.
Origin recognition complex (ORC) binds to replication origins in eukaryotic DNAs and plays an important role replication. Although yeast ORC is known sequence-specifically bind a origin, how human recognizes origin remains unknown. Previous genome-wide studies revealed that guanine (G)-rich sequences, potentially forming G-quadruplex (G4) structures, are present most cells. We previously suggested the region comprising residues 413–511 of subunit 1, hORC1413–511, preferentially G-rich DNAs,...
For the development of K+-responsive RNA aptamers, we proposed a new general strategy that makes use G-quadruplex formation in response to K+. This is first report developing an aptamer demonstrates ON/OFF switching its target-binding activity by sensing addition/removal
Two second-generation quadruplex hammerhead ribozymes, whose activity enhances in response to K(+)via formation of embedded r(GGA)3GG, were developed. Different strategies applied suppress basal when K(+) is absent. As a result, the enhancement upon addition has reached as high 21-fold.