A5038089730- Tuberculosis Research and Epidemiology
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Research and Splicing
- Infectious Diseases and Tuberculosis
- Diagnosis and treatment of tuberculosis
- Mycobacterium research and diagnosis
- Neuroscience of respiration and sleep
- Immune Cell Function and Interaction
- Virology and Viral Diseases
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Mosquito-borne diseases and control
- Neurogenetic and Muscular Disorders Research
- Pancreatic and Hepatic Oncology Research
- Immune cells in cancer
- Viral Infections and Vectors
- Tissue Engineering and Regenerative Medicine
- Chronic Lymphocytic Leukemia Research
- High Altitude and Hypoxia
- Bacterial Genetics and Biotechnology
- Heme Oxygenase-1 and Carbon Monoxide
- CRISPR and Genetic Engineering
Massachusetts General Hospital
2023-2025
Harvard University
2023-2025
Broad Institute
2023-2025
International Centre for Genetic Engineering and Biotechnology
2016-2023
International Centre for Genetic Engineering and Biotechnology
2019
Epidemiological studies suggest that India has the largest number of dengue virus infection cases worldwide. However, there is minimal information about immunological responses in these patients. CD8 T cells are important dengue, because they have been implicated both protection and immunopathology. Here, we provide a detailed analysis HLA-DR+ CD38+ HLA-DR- effector cell subsets patients from Thailand. Both expanded expressed markers indicative antigen-driven proliferation, tissue homing,...
Transcriptional reprogramming of macrophages upon Mycobacterium tuberculosis (Mtb) infection is widely studied; however, the significance alternate splicing (AS) in shaping cellular responses to mycobacterial infections not yet appreciated. Alternate can influence transcript stability or structure, function and localization corresponding proteins thereby altering protein stoichiometry physiological consequences. Using comprehensive analysis a time-series RNA-seq data obtained from human...
Abstract Anti-tuberculosis (TB) drugs, while being highly potent in vitro, require prolonged treatment to control Mycobacterium tuberculosis ( Mtb ) infections vivo. We report here that mesenchymal stem cells (MSCs) shelter help tolerate anti-TB drugs. MSCs readily take up and allow unabated mycobacterial growth despite having a functional innate pathway of phagosome maturation. Unlike macrophage-resident ones, MSC-resident tolerates drugs remarkably well, phenomenon requiring proteins...
Tuberculosis (TB) remains a major global health challenge, particularly in the context of multidrug-resistant (MDR) Mycobacterium tuberculosis (Mtb). Host-directed therapies (HDTs) have been proposed as adjunctive therapy to enhance immune control infection. Recently, one such HDT, pharmacologic modulation myeloid-derived suppressor cells (MDSCs), has treat MDR-TB. While MDSCs well characterized cancer, their role TB pathogenesis unclear. To investigate whether or other myeloid populations...
Mycobacterium tuberculosis is an adaptable intracellular pathogen, existing in both dormant as well active disease-causing states. Here, we report systematic proteomic analyses of four strains, H37Ra, H37Rv, and clinical isolates BND JAL, to determine the differences protein expression patterns that contribute their virulence drug resistance. Resolution lysates strains by liquid chromatography, coupled mass spectrometry analysis, identified a total 2161 groups covering ∼54% predicted M....
Hypobaric Hypoxia (HH) is an established risk factor for various neuro-physiological perturbations including cognitive impairment. The origin and mechanistic basis of such responses however remain elusive. We here combined systems level analysis with classical approaches, in a rat model system, to understand pathological brain HH. Unbiased 'statistical co-expression networks' generated utilizing temporal, differential transcriptome signatures hippocampus—centrally involved regulating...
Summary Previously, we reported that infection of human macrophages with Mycobacterium tuberculosis ( Mtb ) results in massive alterations the pattern RNA splicing host. The finding gained significance since alternate spliced variants a same gene may have substantially different structure, function, stability, interaction partners, localization, and so forth, owing to inclusion or exclusion specific exons. To establish proof‐of‐concept; on how infection‐induced could impact protein...
Secreted virulence factors play a critical role in bacterial pathogenesis. Virulence effectors not only help bacteria to overcome the host immune system but also aid establishing infection. Mtb, which causes tuberculosis humans, encodes various effectors. Triggers that modulate secretion of Mtb are yet be fully understood. To gain mechanistic insight into effectors, we performed high-throughput proteomic studies. With system-level protein-protein interaction network analysis and empirical...
Abstract We earlier reported that Mycobacterium tuberculosis ( Mtb ) alters host RNA splicing to help its intracellular growth. Here, we report an unprecedented mechanism whereby a secreted virulence factor from interferes with the biogenesis of key spliceosomal components, causing altered pattern. A high-throughput yeast-2-hybrid screen identified several -secreted proteins can interact factors (SFs). Through custom-designed in-cell assays, show one those proteins, Rv1435c, targets specific...
Summary Anti-tuberculosis (TB) drugs while being highly potent in vitro require prolonged treatment to control Mycobacterium tuberculosis ( Mtb ) infections vivo . We report here, mesenchymal stem cells (MSCs) shelter help tolerate anti-TB drugs. MSCs uptake readily and allow them grow unabated despite having functional innate pathway of phagosome maturation. Unlike macrophage-resident ones, MSC-resident tolerates remarkably well, a phenomenon requiring proteins ABCC1, ABCG2 vacuolar-type H...