A5063861467- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Protein Degradation and Inhibitors
- Hedgehog Signaling Pathway Studies
- Epigenetics and DNA Methylation
- Prostate Cancer Treatment and Research
- Genomics and Chromatin Dynamics
- Neurogenesis and neuroplasticity mechanisms
- Cell Adhesion Molecules Research
- Pluripotent Stem Cells Research
- Renal and related cancers
- RNA and protein synthesis mechanisms
- Neuroethics, Human Enhancement, Biomedical Innovations
- CRISPR and Genetic Engineering
- Cellular Mechanics and Interactions
- Single-cell and spatial transcriptomics
Université Laval
2020-2025
Université du Québec
2021
Clinical samples are vital for understanding diseases, but their scarcity requires refined research methods. Emerging single-cell technologies offer detailed views of tissue heterogeneity need sufficient fully characterized tissues. We developed an optimized single-nuclei RNA sequencing (snRNA-seq) protocol to extract nuclei from just 15 mg cryopreserved human tissue. Applied four cancer tissues (brain, bladder, lung, prostate), it profiled 1550–7468 per tissue, revealing comparable public...
The recent development of long-read RNA sequencing (lrRNAseq) technologies has paved the way to exploration isoform diversity and its relevance in regulating cell fate plasticity. However, drawing conclusions regarding functionality these newly identified isoforms is still very difficult. Here, we developed a efficient system reprogram mouse cells towards induced pluripotent leveraged strengths lrRNAseq study throughout reprogramming. We several derived from novel splice junctions occurring...
Abstract The cerebral cortex develops from dorsal forebrain neuroepithelial progenitor cells. Following the initial expansion of cell pool, these cells generate neurons all cortical layers and then astrocytes oligodendrocytes. Yet, regulatory pathways that control maintenance pool are currently unknown. Here we define six basic pathway components regulate proliferation cortically specified human stem (cNESCs) in vitro without loss developmental potential. We show activation FGF inhibition...
A recurrent chromosomal translocation found in acute myeloid leukemia leads to an in-frame fusion of the transcription repressor ZMYND11 MBTD1, a subunit NuA4/TIP60 histone acetyltransferase complex. To understand abnormal molecular events that ZMYND11-MBTD1 expression can create, we perform biochemical and functional characterization comparison each individual partner. is stably incorporated into endogenous complex, leading its mislocalization on body genes normally bound by ZMYND11. This...
OPINION article Front. Cell Dev. Biol., 14 July 2020 | https://doi.org/10.3389/fcell.2020.00517
Summary The cerebral cortex develops from dorsal forebrain neuroepithelial progenitor cells. Initial expansion of the cell pool is followed by generation neurons all cortical layers and later, astrocytes oligodendrocytes. However, regulatory pathways that control maintenance are currently unknown. Here we define six basic pathway components regulate proliferation cortically specified human stem cells (cNESCs) in vitro without loss developmental potential. We show activation FGF inhibition...
Emerging evidence associates translation factors and regulators to tumorigenesis. However, our understanding of translational changes in cancer resistance is still limited. Here, we generated an enzalutamide-resistant prostate (PCa) model, which recapitulated key features clinical PCa. Using this model poly(ribo)some profiling, investigated global that occur during acquisition PCa resistance. We found cells exhibit overall decrease mRNA with a specific deregulation the abundance proteins...
The majority of nucleated somatic cells can be reprogrammed to induced pluripotent stem (iPSCs). process reprogramming involves epigenetic remodelling turn on pluripotency-associated genes and off lineage-specific genes. Some evidence shows that iPSCs retain marks their cell origin this "epigenetic memory" influences differentiation potential, with a preference towards origin. Here, we proximal tubule (PTC) tail tip fibroblasts (TTF), from reprogrammable mouse differentiated the renal...
ABSTRACT Emerging evidence associates translation factors and regulators to tumorigenesis. Recent advances in our ability perform global translatome analyses indicate that understanding of translational changes cancer resistance is still limited. Here, we generated an enzalutamide-resistant prostate (PCa) model, which recapitulated key features clinical PCa. Using this model polysome profiling, investigated occur during the acquisition PCa resistance. We found cells exhibit a discordance...
ABSTRACT A chromosomal translocation found in cannibalistic acute myeloid leukemia (AML) leads to an in-frame fusion of the transcription elongation repressor ZMYND11 MBTD1, a subunit NuA4/TIP60 histone acetyltransferase (HAT) complex. In contrast complex, is linked repression actively transcribed genes through recognition H3.3K36me3. To understand abnormal molecular events that expression this ZMYND11-MBTD1 protein can create, we performed its biochemical and functional characterization...