The lectin concanavalin A (Con A) binds methyl alpha-D-mannopyranoside (Me alpha Man) as well alpha-D-mannosyl groups at the nonreducing terminus of oligosaccharides. Ligand peptides that mimic binding Me Man to Con were identified from screening an epitope library composed filamentous phage displaying random hexapeptides. consensus sequence was among affinity-purified phage; bearing this and is inhibited doing so by Man. When tested for against a panel lectins, bind only weakly closely...

Significance The current cleanroom-based soft lithography microfabrication process is complicated and expensive. There a need for low-cost, ready-to-use, modular components that can be easily assembled into microfluidic devices by users lacking proficiency or access to facilities. We present facile, efficient method of fabricating soft, elastic microtubes with different cross-sectional shapes dimensions. These used as basic building blocks the rapid construction various 2D 3D complex...

In recent years, there has been a rise in the incidence of renal cell carcinoma (RCC), with metastatic RCC being prevalent and significant contributor to mortality. While regulatory role for microRNAs (miRNAs) development progression recognized, their precise functions, molecular mechanisms, potential clinical implications remain inadequately elucidated. Hence, this study aimed explore miR-507 identify STEAP3 as downstream target miR-507. Bioinformatics analysis was used analyze expression...

The distribution and location of mast cells are closely related to their physiological pathological functions, such as allergic responses, immunity, fibrosis, used in acupuncture.In this study, the vivo was observed, mechanical clues for understanding based on niches were explored.By toluidine blue staining immunohistochemical staining, we examined rat skin found that distributed a spatially nonuniform manner, preferring locate at regions tissue extracellular matrix with stiffness changes.In...

Mechanical properties of red blood cells (RBCs) change during their senescence which supports numerous physiological or pathological processes in circulatory systems by providing crucial cellular mechanical environments hemodynamics. However, quantitative studies on the aging and variations RBC are largely lacking. Herein, we investigate morphological changes, softening stiffening single RBCs using an vitro fatigue model. Using a microfluidic system with microtubes, repeatedly subjected to...

This paper attempts to introduce an improved difference model that modifies a car-following model, which takes the next-nearest-neighbor interaction into account. The improvement of this over previous one lies in it performs more realistically dynamical motion for small delay time. traffic behavior is investigated with analytic and numerical methods finding new consideration could further stabilize flow. And some simulation tests verify proposed can demonstrate complex physical features...

The arterial mechanical microenvironment, including stiffness, is a crucial pathophysiological feature of vascular remodeling, such as neointimal hyperplasia after carotid endarterectomy and balloon dilatation surgeries. In this study, we examined changes in stiffness Sprague-Dawley rat artery intimal injury model revealed that extracellular matrix (ECM) secretion were increased. Once the endothelial layer damaged vivo , activated platelets adhere to intima may secrete platelet-derived...

Non-muscle myosin II (NMII) is a force-generating mechanosensitive enzyme that responds to mechanical forces. NMIIs mechanoaccumulate at the cell cortex in response It essential for cells mechanically adapt physical environment, failure of which results mitotic defects when dividing confined environment. Much less known about how NMII mechanoaccumulation regulated during mitosis. We show respond compressive stress by promoting accumulation active RhoA as interphase cells. mechanoresponse...

Abstract A novel full Eulerian fluid-elastic membrane coupling method on the fixed Cartesian coordinate mesh is proposed within framework of volume-of-fluid approach. The present based a fluid-(bulk) structure solver (Sugiyama et al., J. Comput. Phys ., 230 (2011) 596-627), with bulk replaced by elastic membranes. In this study, closed consid-ered, and it described or volume-fraction information generally called VOF function. smoothed indicator (or characteristic) function introduced as...

The equilibrium positions of red blood cells (RBCs) and their steady motions in microchannel affect the hemodynamics vivo microfluidic applications on a cellular scale. However, dynamic behavior single RBC three-dimensional cylindrical microchannels still needs to be classified systematically. Here, with an immersed boundary method, phase diagrams profiles RBCs under states are illustrated wide range Capillary numbers. effects initial explored as well. Numerical results present that at...

The deformation of multiple red blood cells in a capillary flow was studied numerically. immersed boundary method used for the fluid interaction. membrane cell modeled as hyperelastic thin shell. numerical results show that apparent viscosity is more sensitive to change shear coefficient than bending and surface dilation coefficient, increase an pressure drop vessels order sustain same flux rate cells.

In this paper, we discuss the motion of a vesicle in linear shear flow. It is known that deformable vesicles such as liposomes show so-called tank-treading and tumbling motions depending on viscosity ratio between inside outside vesicle, swelling ratio, so on. First, have conducted numerical simulations liposome flow compared results with other experimental results. confirmed inclination angle becomes smaller when larger or present quantitatively good agreement Then, effects membrane...

The high-pressure multistage centrifugal pump is the main piece of energy-consuming equipment in reverse osmosis desalination process, and it consumes about 35% entire system’s operating cost. optimization process multi-stage pumps undoubtedly requires performance comparison multiple schemes order to verify effectiveness optimized design method. Therefore, based on ANSYS Workbench an improved bat algorithm, intelligent scheme was designed carried out a three-stage for efficiency improvement...

Abstract To reduce the energy consumption of large centrifugal pumps, modified heuristic intelligent algorithms are used to directly optimize diffuser pumps. Considering hydraulic efficiency under design condition as optimization target, in this study, 14 geometric parameters such inlet diameter, outlet and leading trailing vane angles selected variables, particle swam gravitational search algorithm for space. The performance loss internal entropy production different models before after...

Mast cells (MCs) play important roles in multiple pathologies, including fibrosis; however, their behaviors different extracellular matrix (ECM) environments have not been fully elucidated. Accordingly, this study, the migration of MCs on substrates with stiffnesses was investigated using time-lapse video microscopy. Our results showed that could appear round, spindle, and star-like shapes; spindle-shaped accounted for 80-90 % total observed cells. The speed round significantly lower than...

The membrane tensions of suspended nucleated cells moving in blood flows capillary networks are quite different from those spreading cells, a fact that is crucial to many pathological processes, such as the metastasis cancers via circulating tumor (CTCs). However, few studies have examined especially when interacting with other stiffnesses low-Reynolds number at cellular level. Taking CTCs an example, we use immersed boundary method analyze relationship between and their motional behaviors...

The influences of uncertainties in nuclear physics inputs on the Th/U, Th/Hf, Th/Eu, Th/Os, Th/Ir nucleo-chronometers are investigated framework classical r-process approach. A Monte-Carlo method is used to evaluate age uncertainty originating from neutron separation energies for each mass model. It found that deduced Th /U can be up 1.66 Ga, and three chronometers, Th/Ir, 5.15 3.93 Ga 3.95 respectively. recently proposed chronometer, shows a clear model dependence, while Th/Os chronometers...