A5100633910- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Chemical Synthesis and Reactions
- Enzyme Structure and Function
- Advanced biosensing and bioanalysis techniques
- Polyoxometalates: Synthesis and Applications
- CRISPR and Genetic Engineering
- RNA regulation and disease
- Particle physics theoretical and experimental studies
- Dark Matter and Cosmic Phenomena
- Polymer crystallization and properties
- DNA and Nucleic Acid Chemistry
- Gold and Silver Nanoparticles Synthesis and Applications
- Polymer composites and self-healing
- RNA Interference and Gene Delivery
- Cosmology and Gravitation Theories
- Microstructure and Mechanical Properties of Steels
- Transition Metal Oxide Nanomaterials
- Carbon dioxide utilization in catalysis
- Conducting polymers and applications
- Protein Degradation and Inhibitors
- Mesoporous Materials and Catalysis
- Aluminum Alloy Microstructure Properties
Zhengzhou University
2015-2025
University of Chinese Academy of Sciences
2016-2025
Shenzhen Institutes of Advanced Technology
2023-2025
Wuhan Children's Hospital
2024
Huazhong University of Science and Technology
2024
Northwestern Polytechnical University
2014-2024
Jiangsu Province Hospital
2024
Chinese Academy of Sciences
2014-2024
Nanjing Medical University
2024
Jiaozuo University
2024
We describe the design and synthesis of a cyanine-based near-infrared ratiometric fluorescent probe, HS–Cy, for H2S detection, which features rapid response, high sensitivity, mitochondria targeting. After quenching at 780 nm by initial nucleophilic addition on aldehyde group, HS–Cy experienced polymethine π-electron conjugation modulation triggered second ester, releasing cyanine fluorophore underwent tautomerism from enol form to ketone form. Therefore, gradual emergence new peak 625 was...
The type V-F CRISPR-Cas12f system is a strong candidate for therapeutic applications due to the compact size of Cas12f proteins. In this work, we identify six uncharacterized Cas12f1 proteins with nuclease activity in mammalian cells from assembled bacterial genomes. Among them, OsCas12f1 (433 aa) Oscillibacter sp. and RhCas12f1 (415 Ruminiclostridium herbifermentans, which respectively target 5' T-rich Protospacer Adjacent Motifs (PAMs) C-rich PAMs, show highest editing activity. Through...
Acute myeloid leukemia (AML) is marked by significant unmet clinical need due to both poor survival and high relapse rates where long-term disease control for most patients with relapsed or refractory AML remain dismal. Inspired bring novel therapeutic options these patients, we envisioned protein degradation as a potential approach the treatment of AML. Following this course, discovered pioneered mechanism action which culminated in discovery CC-90009. CC-90009 represents degrader first...
Abstract Transposon-associated ribonucleoprotein TnpB is known to be the ancestry endonuclease of diverse Cas12 effector proteins from type-V CRISPR system. Given its small size (408 aa), it interest examine whether engineered could used for efficient mammalian genome editing. Here, we showed that gene editing activity native Deinococcus radiodurans (ISDra2 TnpB) in mouse embryos was already higher than previously identified small-sized Cas12f1. Further stepwise engineering noncoding RNA...
To understand the interplay of residual structures and conformational fluctuations in interaction intrinsically disordered proteins (IDPs), we first combined implicit solvent replica exchange sampling to calculate atomistic ensembles nuclear co-activator binding domain (NCBD) transcription coactivator CBP activation p160 steroid receptor ACTR. The calculated are quantitative agreement with NMR-derived residue helicity recapitulate experimental observation that, while free ACTR largely lacks...
Graphene oxides were grafted with functionalized ionic liquid and showed high catalytic activity in cycloaddition of CO<sub>2</sub>with propylene oxide to carbonate.
Achieving facile specific recognition is essential for intrinsically disordered proteins (IDPs) that are involved in cellular signaling and regulation. Consideration of the physical time scales protein folding diffusion-limited protein-protein encounter has suggested frequent requirement IDP could lead to kinetic bottlenecks. How IDPs overcome such potential bottlenecks viably function regulation general poorly understood. Our recent computational experimental study cell-cycle regulator p27...
The hydrogenation process of the layered α-MoO3 crystal was investigated on a nanoscale. At low hydrogen concentration, can lead to formation HxMoO3 without breaking MoO3 atomic flat surface. For with high atoms accumulated along <101> direction MoO3, which induced oxygen vacancy line defects. injected acted as electron donors increase electrical conductivity MoO3. Near-field optical measurements indicated that both and vacancies were responsible for coloration hydrogenated latter...
We previously disclosed the identification of cereblon modulator 3 (CC-885), with potent antitumor activity mediated through degradation GSPT1. describe herein structure-activity relationships for analogs exploration structurally related dioxoisoindoline class. The observed protein could in part be rationalized docking into 3-CRBN-GSPT1 cocrystal ternary complex. For SAR that not complex, we sought to predict a QSAR model developed house. Through these analyses, selective achieved between...
An <italic>in situ</italic> Raman study of the mechanism HER catalytic performance enhanced by high-index facets on Ti@TiO<sub>2</sub> nanosheets.
Abstract The delivery of biomolecules by extracellular vesicles (EVs) derived from endothelial progenitor cells (EPCs) has been proven to ameliorate sepsis, yet the therapeutic mechanism remains be elucidated. Taurine upregulated gene 1 ( TUG1 ) is a long noncoding RNA (lncRNA) that downregulated in sepsis. current study was designed explore role EPCs EVs transmitting macrophage polarization and macrophage-mediated inflammation cecal ligation puncture (CLP)-induced sepsis mouse model....
New organic heteropolyacid (HPA) salts prepared by exchanging counter protons of Keggin HPAs with N-methyl-2-pyrrolidonium proved to be highly efficient, conveniently recoverable, and steadily reusable heterogeneous catalysts for the solvent-free Prins reactions styrenes formalin.
Abstract As an important part of tumor microenvironment, neutrophils are poorly understood due to their spatiotemporal heterogeneity in tumorigenesis. Here we defined, at single-cell resolution, CD44 −CXCR2 − as tumor-specific (tsNeus) both mouse and human gastric cancer (GC). We uncovered a Hippo regulon with unique YAP signature genes (e.g., ICAM1, CD14, EGR1) distinct from those identified epithelial and/or cells. Importantly, knockout YAP/TAZ neutrophiles impaired differentiation into...
Abstract Proteins are the building blocks of life, carrying out fundamental functions in biology. In computational biology, an effective protein representation facilitates many important biological quantifications. Most existing methods derived from self‐supervised language models designed for text analysis. Proteins, however, more than linear sequences amino acids. Here, a multimodal deep learning framework incorporating ≈1 million sequence, structure, and functional annotation (MASSA) is...
ABSTRACT To explore the chain mediating role of social support and trust in between decision self‐efficacy conflict stroke caregivers. Convenient sampling was used to select caregivers who were admitted department neurology, neurosurgery rehabilitation a four‐3A hospital Henan Province from September 2023 April 2024. General information questionnaire, Decisional Self‐efficacy Scale, Social Support Wake Forest Physician Trust Scale Conflict adopted. Using SPSS plug‐in PROCESS analyze effect,...
Intrinsically disordered proteins (IDPs) lack stable structures under physiological conditions but often fold into upon specific binding. These coupled binding and folding processes underlie the organization of cellular regulatory networks, a mechanistic understanding is thus fundamental importance. Here, we investigated synergistic two IDPs, namely, NCBD domain transcription coactivator CBP p160 steroid receptor ACTR, using topology-based model that was carefully calibrated to balance...
The first data from the LHC Run-2 have shown a possible excess in diphoton events with invariant mass $\sim 750$ GeV, suggesting existence of new resonance which may decay dominantly into dark matter (DM) particles. We show simple model that reported at is consistent another photon excess, $2$ GeV cosmic gamma-ray fluxes towards Galactic Center observed by Fermi-LAT. Both excesses can be simultaneously explained 60$ scalar DM particle annihilating two gluons typical thermal annihilation...