A5004302690- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- RNA regulation and disease
- Advanced biosensing and bioanalysis techniques
- Peer-to-Peer Network Technologies
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Multimedia Communication and Technology
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Semantic Web and Ontologies
- Usability and User Interface Design
- RNA Research and Splicing
Sun Yat-sen University
2007-2011
Abstract Transposon-associated ribonucleoprotein TnpB is known to be the ancestry endonuclease of diverse Cas12 effector proteins from type-V CRISPR system. Given its small size (408 aa), it interest examine whether engineered could used for efficient mammalian genome editing. Here, we showed that gene editing activity native Deinococcus radiodurans (ISDra2 TnpB) in mouse embryos was already higher than previously identified small-sized Cas12f1. Further stepwise engineering noncoding RNA...
Duchenne muscular dystrophy (DMD) affecting 1 in 3500-5000 live male newborns is the frequently fatal genetic disease resulted from various mutations DMD gene encoding dystrophin protein. About 70% of DMD-causing are exon deletion leading to frameshift open reading frame and deficiency. To facilitate translating human DMD-targeting CRISPR therapeutics into patients, we herein establish a genetically humanized mouse model by replacing 50 51 Dmd with sequence. This recapitulats patient's...
Identification of aberrant microRNA (miRNA) expression during chemical carcinogen–induced cell transformation will lead to a better understanding the substantial role miRNAs in cancer development. To explore whether can be used as biomarkers exposure risk assessment carcinogenesis, we analyzed miRNA profiles human bronchial epithelial cells expressing an oncogenic allele H-Ras (HBER) at different stages induced by benzo(a)pyrene (BaP) array. It revealed 12 differentially expressed HBER both...
IscBs, as hypercompact ancestry proteins of Cas9 nuclease, are suitable for in vivo gene editing via single adeno-associated virus (AAV) delivery. Due to the low activity natural IscBs eukaryotic cells, recent studies have been focusing on improving OgeuIscB's efficiency protein engineering. However, efficacy disease correction remained be demonstrated. Here, we showed effective knockout and base mouse embryos. To further improve IscB activity, performed systematic engineering...
Concurrency control strategy is important and necessary to Collaborative Authoring System (CAS) which allows several users edit a document simultaneously. Locking algorithm adopted in traditional CAS can't achieve excellent concurrency with high performance. In this paper we propose an adaptive-granularity locking algorithm, determines lock granularity by means of user's operations dynamically. The experiments have carried out show that our algorithms the fine-granularity lower cost.