A5101545443- Soybean genetics and cultivation
- Cholinesterase and Neurodegenerative Diseases
- Microbial Natural Products and Biosynthesis
- Pharmacological Effects of Natural Compounds
- Nanoplatforms for cancer theranostics
- Chemical synthesis and alkaloids
- Cancer therapeutics and mechanisms
- Insect Resistance and Genetics
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Phytochemistry and Bioactivity Studies
- Molecular Biology Techniques and Applications
- Nanoparticle-Based Drug Delivery
- Pesticide Exposure and Toxicity
- Plant pathogens and resistance mechanisms
- Oxidative Organic Chemistry Reactions
- Fungal Biology and Applications
- Influenza Virus Research Studies
- Photoacoustic and Ultrasonic Imaging
- Animal Disease Management and Epidemiology
- RNA and protein synthesis mechanisms
- Biochemical and Molecular Research
- Essential Oils and Antimicrobial Activity
- Ginseng Biological Effects and Applications
- Genetically Modified Organisms Research
Chinese PLA General Hospital
2024-2025
China Jiliang University
2024
Chinese Academy of Sciences
2017-2023
Center for Excellence in Molecular Plant Sciences
2017-2023
Donghua University
2022
Jimei University
2020
Shanghai Institutes for Biological Sciences
2017-2019
State Key Laboratory of Plant Genomics
2017
Institute of Genetics and Developmental Biology
2017
Fujian University of Traditional Chinese Medicine
2015
Soybean (Glycine max [L.] Merr.) is one of the most important oil and protein crops. Ever-increasing soybean consumption necessitates improvement varieties for more efficient production. However, both correlations among different traits genetic interactions genes that affect a single trait pose challenge to breeding. To understand networks underlying phenotypic correlations, we collected 809 accessions worldwide phenotyped them two years at three locations 84 agronomic traits. Genome-wide...
Sonodynamic therapy (SDT) is one of the promising strategies for tumor therapy, but its application usually hindered by fast clearance in blood-circulation, abnormal microenvironment, and inefficient generation reactive oxygen species. To solve these problems, we proposed an on-demand assembly-disassembly strategy, where assembly favorable longer-blood-circulation then disassembly boosting SDT. Hematoporphyrin monomethyl ether (HMME) as model organic sonosensitizers were conjugated with...
Activatable polymeric nanosystems have attracted great interest, and their interactions with endo-exogenous stimulations are highly vital for therapeutic efficacy, which urgently needs systematic study. Herein we focus on systematically investigating these an enzyme-nanosystem model, the tumor-overexpressed hyaluronidase (HAase) doxorubicin-loaded hyaluronic-acid-porphyrin nanoassemblies (DOX@HPNAs), to augment photo-sono-chemo therapies. The HAase degrades HPNAs in acidic solution at a...
Duchenne muscular dystrophy (DMD) affecting 1 in 3500-5000 live male newborns is the frequently fatal genetic disease resulted from various mutations DMD gene encoding dystrophin protein. About 70% of DMD-causing are exon deletion leading to frameshift open reading frame and deficiency. To facilitate translating human DMD-targeting CRISPR therapeutics into patients, we herein establish a genetically humanized mouse model by replacing 50 51 Dmd with sequence. This recapitulats patient's...
Compound identification is a key component of data analysis in the applications gas chromatography–mass spectrometry (GC-MS). Currently, most widely used compound mass spectrum matching, which dot product and its composite version are employed as spectral similarity measures. Several forms transformations for fragment ion intensities have also been proposed to increase accuracy identification. In this study, we introduced partial semipartial correlations measures applied them identify...
Huperzia serrata (H. serrata) is an economically important traditional Chinese herb with the notably medicinal value. As a representative member of Lycopodiaceae family, H. produces various types effectively bioactive lycopodium alkaloids, especially huperzine A (HupA) which promising drug for Alzheimer's disease. Despite their importance, public genomic and transcriptomic resources are very limited biosynthesis HupA largely unknown. Previous studies on comparison 454-ESTs from Phlegmariurus...
Securinega alkaloids represent a class of plant secondary metabolites with intriguing bridged tetracyclic structures and promising biological activities. Despite extensive synthetic efforts number innovative total syntheses, no relevant biosynthetic genes have been reported to date. In this study, fluesuffine A (1), C-2 C-3 functionalized derivative fused 5/5/5/6/5/6/5 congested heptacyclic ring system, was isolated from Flueggea suffruticosa. the biogenetic study 1, we found berberine...
Qian ceng Ta, the whole plant of Huperzia serrata, is an important landscape and medicinal herbs contains abundant bioactive lycopodium alkaloids. Although structures more than 100 alkaloids in serrata have been isolated identified, content distribution these different tissues are still unclear. In current study, ultra-performance liquid chromatography-mass spectrometry based comprehensive metabolomics strategy was developed, including extraction, separation, identification, statistical...
Huperzine A (HupA) is a powerful and selective inhibitor of acetylcholinesterase. It has attracted widespread attention endangering the ultimate plant sources Lycopodiaceae family. In this study, we used Huperzia serrata, extensively in Traditional Chinese medicine (TCM), slow growing vascular as model family to develop validate reference genes. We aim use gene expression platform understand different tissues developmental stages medicinal herb. Eight candidate genes were selected based on...
Aims To search for a set of reference genes reliable gene expression analysis in the globally important marine coccolithophore Emiliania huxleyi–virus model system. Methods and Results Fifteen housekeeping (CDKA, CYP15, EFG3, POLAI, RPL30, RPL13, SAMS, COX1, GPB1-2, HSP90, TUA, TUB, UBA1, CAM3 GAPDH) were evaluated their stability as potential qRT-PCR using ΔCt, geNorm, NormFinder, Bestkeeper RefFinder software. CDKA, TUA TUB tested loading controls Western blot same sample panel....
The H5N1 avian influenza virus seriously affects the health of poultry and humans. Once infected, mortality rate is very high. Therefore, accurate timely detection beneficial for controlling its spread. This article establishes a dual gene method based on RPA simultaneously detecting HA M2 genes virus, virus. Design specific primers conserved regions genes. sensitivity RT-RPA 1 × 10−7 ng/μL. optimal primer ratio 1:1, reaction temperature 40 °C, time 20 min. Dual was used to detect 72...
Abstract Camptothecin and its derivatives are the third largest anticancer drugs in world market, mainly used to treat malignant tumors such as lung, colon cervical cancer. was firstly discovered Camptotheca acuminate extracted from C. Nothapodytes nimmoniana for medicine production (Sadre et al. 2016). However, overharvesting of N. has greatly reduced their populations nature, which currently listed second protected plants China India. It is estimated there would be 20 million new cancer...
Securinega alkaloids represent a class of plant secondary metabolites with intriguing bridged tetracyclic structures and promising biological activities. Despite extensive synthetic efforts and number innovative total syntheses, no relevant biosynthetic genes have been reported to date. In this study, fluesuffine A (1), C-2 and C-3 functionalized derivative fused 5/5/5/6/5/6/5 congested heptacyclic ring system, was isolated from Flueggea suffruticosa. the biogenetic study 1, we found...