A5032479200- Neuropeptides and Animal Physiology
- Osteoarthritis Treatment and Mechanisms
- Peptidase Inhibition and Analysis
- Cell Adhesion Molecules Research
- Inflammatory mediators and NSAID effects
- Protease and Inhibitor Mechanisms
- Immune Cell Function and Interaction
- Cancer, Stress, Anesthesia, and Immune Response
- Immunotherapy and Immune Responses
- Helicobacter pylori-related gastroenterology studies
- Total Knee Arthroplasty Outcomes
- Asthma and respiratory diseases
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Chemokine receptors and signaling
- Rheumatoid Arthritis Research and Therapies
- Signaling Pathways in Disease
- Cytokine Signaling Pathways and Interactions
Universidad Complutense de Madrid
2016-2025
Research Institute Hospital 12 de Octubre
2018-2020
<title>Abstract</title> Current therapies for osteoarthritis (OA) focus on symptom management, rather than halting disease progression. Vasoactive intestinal peptide (VIP) has shown promising effects in musculoskeletal diseases, preserving joint integrity and modulating inflammation. This study investigates the potential of VIP to promote chondrogenic differentiation human bone marrow mesenchymal stem cells (BM-hMSC), while inflammatory cartilage extracellular matrix (ECM)-degrading...
Failure of therapeutic approaches for the treatment osteoarthritis (OA) based on inhibition metalloproteinases, might be because their constitutive expression in homeostasis, together with network complexity. The knowledge this would contribute to selective target pathological conditions. In sense, blockade mediators produced by neighbouring joint cells, such as synovial fibroblasts (SF), prevent cartilage damage. Thus, we studied contribution ADAMTS-7 and -12 from SF oligomeric matrix...
Abstract Several neuropeptides present in bone tissues, produced by nerve fibers and cells, have been reported to play a role regulating the fine‐tuning of osteoblast osteoclast functions maintain homeostasis. This study aims characterize influence neuropeptide vasoactive intestinal peptide (VIP) on differentiation process human mesenchymal stem cells (MSCs) into osteoblasts their anabolic function. We describe mRNA protein expression profile VIP its receptors MSCs as they differentiate...
Osteoarthritis (OA) is the most common musculoskeletal disorder causing a great disability and reduction in quality of life. In OA, articular chondrocytes (AC) synovial fibroblasts (SF) release innate-derived immune mediators that initiate perpetuate inflammation, inducing cartilage extracellular matrix (ECM) degradation. Given lack therapies for treatment this study, we explore biomarkers enable development new therapeutical approaches. We analyze set secreted proteins AC SF co-cultures by...
Vasoactive Intestinal Peptide (VIP) is an important immunomodulator of CD4+ cells in normal and pathological conditions, which exerts its anti-inflammatory immunomodulatory actions through VPAC receptors, VPAC1 VPAC2. Only a decrease the expression mRNA on Th upon activation has been reported. Thus, deepening knowledge behavior these receptors may contribute to design new therapies based their and/or blockade. In this study, we describe pattern, cellular location functional role VIP during...
Few studies have considered immune-mediated inflammatory disorders (IMID) together, which is necessary to adequately understand them given they share common mechanisms. Our goal was investigate the expression of vasoactive intestinal peptide (VIP) and its receptors VPAC1 VPAC2 in selected IMID, analyze effect biological therapies on them, identify miRNA signatures associated with their expression. Serum VIP levels mRNA VPAC peripheral blood mononuclear cells were analyzed from 52 patients...
Pro-inflammatory CD4+CD28− T cells are characteristic of immunosenescence, but also several autoimmune/inflammatory diseases. Vasoactive intestinal peptide (VIP) acts as an anti-inflammatory and immunomodulatory mediator on these cells. Our objective was to study the mutual influence between senescent Th VIP axis in early arthritis (EA), comparing with non-EA donors. We characterized correlation clinic parameters EA well behavior biomarkers by real-time PCR. Clinical data were systematically...
We aimed to evaluate the direct action of VIP on crucial molecules involved in human osteoclast differentiation and function. also investigated relationship between serum levels bone remodeling mediators early arthritis patients. The expression receptors gene markers monocytes vitro differentiated osteoclasts was studied by real-time PCR. NFATc1 activity measured using a TransAM® kit. Osteoclastogenesis confirmed quantification tartrate-resistant acid phosphatase positive multinucleated...
Naїve CD4+ T cells, which suffer different polarizing signals during cell receptor activation, are responsible for an adequate immune response. In this study, we aimed to evaluate the behavior of human CD4+CD45RA+ cells after in vitro activation by anti-CD3/CD28 bead stimulation 14 days. We also wanted check role VIP system process. The metabolic biomarker Glut1 was increased, pointing increase glucose requirement whereas Hif-1α expression higher resting than activated cells. Expression Th1...
<h3>Background</h3> Proteinases released from synovial membrane of osteoarthritis (OA) patients contribute to cartilage damage. We recently reported in fibroblasts (SF) the expression ADAMTS (a disintegrin and metalloproteinase domain with thrombospondin motifs)-7 -12, involved destruction oligomeric matrix protein (COMP) (1). Signaling pathways regulating these are poorly understood. As Runx2 β-catenin two transcription factors chondrocytes metabolism OA pathology (2–5), we studied whether...