ABSTRACT Salmon ( Salmo salar ) was stored in ice up to 24 d, and changes during storage were observed with sensory evaluation using the Quality Index Method (QIM), Quantitative Descriptive Analysis (QDA), total viable counts (TVC), hydrogen sulfide (H2S)‐producing bacteria, instrumental texture measurements (compression test). Maximum time determined QDA fat content by Soxhlet extraction. A high correlation between QIM found. Storage could be predicted ± 2 d. TVC increased exponentially...

The individual amino acid contacts responsible for peptide binding to DRB1*0101 and/or DRB1*0401 were defined using a quantitative assay. differential contribution of each in two well studied T cell determinants, HA307-319 and RMBP 90-102, was delineated by comparing the IC50 values analogues varying length. This analysis confirmed importance hydrophobic located near amino-terminus both alleles revealed that carboxyl-terminal acids differed between DRB1*0401. Taking advantage previous...

AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 61:179-189 (2010) - DOI: https://doi.org/10.3354/ame01443 Latitudinal patterns in abundance of major marine bacterioplankton groups Matthias Wietz1,*, Lone Gram1, Bo Jørgensen1, Andreas Schramm2 1National Food Institute, Technical University Denmark, 2800 Kgs. Lyngby, Denmark 2Department Biological Sciences,...

Analysis of peptide binding to a set HLA-DR alleles has allowed the proteins be segregated into functional subsets, depending on amino acids at positions 57 and 86 beta-chain. DR with glycine beta aspartic acid bound simplified significantly lower IC50 values than that did not have this combination acids. The size seemed modify steric requirements for single most important side chain peptide. Within each four subgroups, other polymorphic define allele-specific requirements. These were...

The accumulation of formaldehyde and the resulting deterioration seafood products during frozen storage are primarily caused by enzymatic activity trimethylamine oxide aldolase (TMAOase). A screening muscle samples from 24 species showed TMAOase in only nine gadiform that were analyzed. Enzyme activities major white fish large variations between as well individuals. experiment a similarly variation rate accumulation, which could be accounted for endogenous situ activity. This also correlated...

Five methods for finding significant changes in proteome data have been used to analyze a two-dimensional gel electrophoresis set. We both univariate (ANOVA) and multivariate (Partial Least Squares with jackknife, Cross Model Validation, Power-PLS CovProc) methods. The gels were taken from time-series experiment exploring the metabolic enzymes bovine muscle at five time-points after slaughter. set consisted of 1377 protein spots, each analysis, preprocessed fit requirements chosen method....

Abstract The effect of frozen storage temperature on quality-related parameters rainbow trout (Oncorhynchus mykiss) muscle was studied in the interval from −10 to −80°C samples stored for 1 18 months. following quantities were measured: drip loss, water holding capacity and distribution, color, lipid oxidation (thiobarbituric acid-reactive substances, TBARS), membrane stability (enzyme activity). No capacity, or observed below −20°C, whereas at −40°C lower compared −30°C higher resulted a...

SummaryThe ability of a variety common intestinal microrganisms to grow in bile salt-containing media and alter the structure those salts was studied. Strains Escherichia coli, Proteus vulgaris, Streptococcus faecalis were adapted by serial transfer cholate-containing more rapidly high concentrations cholate composition than unadapted cultures. E. coli also shown deoxycholate. Low linoleic or palmitic acids when added partially overcame inhibition growth cholate-altering capacity coli....

Practical approaches to the use of multivariate data analysis 2-DE protein patterns are demonstrated by three independent strategies for image and on same set data. Four wheat varieties were selected basis their baking quality. Two strong quality hard kernel two weak soft kernel. Gliadins at different stages grain development analyzed application images 2-DEs. Patterns related varieties, harvest times detected all strategies. The methods was evaluated in alignment matching procedures gels....

Abstract Parallel factor analysis (PARAFAC) is a widespread method for modeling fluorescence data by means of an alternating least squares procedure. Consequently, the PARAFAC estimates are highly influenced outlying excitation–emission landscapes (EEM) and element‐wise outliers, like example Raman Rayleigh scatter. Recently, robust that circumvents harmful effects samples has been developed. For removing scatter on final model, different techniques exist. Newly, automated identification...

Cod (Gadus morhua) was stored at eight temperatures (−10 to −80°C) for 1 18 months, after which quality indicators were measured—including drip loss, water holding capacity, low field NMR spin-spin relaxation, color, amount of thiobarbituric acid reactive substances, and sarcoplasmic reticulum Ca2+-ATPase lysosomal Cathepsin D activities. Results from samples up 12 months showed no significant differences between −30°C lower temperatures. The measurements, however, indicated some changes in...

Two-dimensional gel electrophoresis (2-DE) produces large amounts of data and extraction relevant information from these demands a cautious time consuming process spot pattern matching between gels. The classical approach analysis is to detect protein markers that appear or disappear depending on the experimental conditions. Such biomarkers are found by comparing relative volumes individual spots in Multivariate statistical modelling 2-DE for comparison classification an alternative...

One application of 2D gel electrophoresis is to reveal differences in protein pattern between two or more groups individuals, attributable their group membership. Multivariate data analytical methods are useful pinpointing the spots relevant for discrimination by focusing not only on single spot differences, but covariance structure proteins. However, outcome dependent scaling, and they may fail producing valid multivariate models due much higher number "irrelevant" present gels. The case...

Intact pigeon heart mitochondria showed 10-30% ubiquinone reduction in the absence of substrates. This could not be ascribed to endogenous substrates, as judged by lack effect inhibitors and uncouplers very low respiratory rate. Addition NADH presence antimycin caused further about 10% ubiquinone, apparently coupled rotenone- antimycin-sensitive exo-NADH oxidase system [Rasmussen (1969) FEBS Lett. 2, 157-162]. Citric acid cycle substrates reduced most remaining antimycin; 15-20% total...

Abstract Low‐field 1 H nuclear magnetic resonance transverse relaxation profiles were measured on muscle samples from herring differing in catch season (summer or winter), fishing ground (Baltic Sea, North Skagerrak Kattegat), biological parameters (maturity status, sex and spawning type), lipid water content, catching vessel (the institute's research Dana commercial vessels). Water mobility distribution calculated the NMR signals by use of multi‐way analysis. The caught differed according...

Near infrared diffuse reflectance spectra were recorded from the skin of 115 thawed whole cod (Gadus morhua) varying quality. In fish fillets same four frozen quality parameters (water holding capacity, concentration total volatile nitrogen bases, dimethylamine, and formaldehyde) determined. A principal component analysis showed that strongly correlated with each other. Partial least squares (PLS) regression models based on full variables, respectively, compared respect to prediction ability...