The aim of this study was to evaluate the nutritional quality protein from Moringa oleifera seeds and leaves. defatted flours were rich in (33.53 18.63% for leaves, respectively) carbohydrates. Amino acid analysis revealed presence all essential amino acids both leaf seed flour, with high content leucine valine low methionine cysteine. total flour (42.76 g/16 g N) higher than that (35.07 N). Limiting lysine sulfur acids. available (3.78 significantly (1.30 In vitro digestibility studies...

In this study, we studied the bioactive peptides produced by thermolysin hydrolysis of a water-soluble protein (WSP) from red alga Gracilariopsis chorda, whose major components are phycobiliproteins and Ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCo). The results showed that WSP hydrolysate exhibited significantly higher ACE inhibitory activity (92% inhibition) compared to DPP-IV DPPH scavenging activity. RuBisCo G. chorda contain high proportion hydrophobic (31.0-46.5%) aromatic...

Cowhide collagen hydrolysates (CCHs) are peptides and amino acids obtained from the partial hydrolysis of collagen. These have numerous potential applications in food, biomedical, pharmaceutical industries. The study analyzed physicochemical, antioxidant, anti-atherosclerosis properties cowhide using silico methods. Proteins were identified based on their molecular weights origin protein database (UniProtKB). Using bioinformatics tools, physicochemical (toxicity acid composition) determined....

Effect of cabinet-drying, vacuum-drying, and freeze-drying methods on the surface hydrophobicity, secondary structure, emulsifying, foaming properties protein concentrates prepared from different cultivars cowpea Bambara bean was investigated. The vacuum-drying method reduced while freeze-dried presented high hydrophobicity. by freeze drying more β-sheet (40–43%) less β-turn (19–24%) structures. higher emulsifying activity (56–59%) compared to those varied significantly among (46–61%)....

In this study, the α-glucosidase (maltase-glucoamylase: MGAM) and α-amylase inhibitory properties elicited by xylooligosaccharides (XOSs) prepared from dulse xylan were analysed as a potential mechanism to control postprandial hyperglycaemia for type-2 diabetes prevention treatment. Xylan was purified red alga powder used enzymatic hydrolysis using Sucrase X produce XOSs. Fractionation of XOSs produced xylobiose (X2), β-(1→3)-xylosyl (DX3), xylotriose (X3), β-(1→3)-xylosyl-xylotriose (DX4),...

Bambara bean is a rich low-cost protein source and functional ingredient in the food industry. We investigated effects of temperature different pH on physicochemical properties isolate. Vicilin was major as revealed by SDS PAGE analysis. The emulsifying capacity isolate highest at 80 °C, 9 while emulsion stability 4. Generally, increase decreased solubility 4 7, observed 100 °C. hydrophobicity lowest 9, regardless temperature. Protein possessed highly compact β-sheet α-helix secondary...

The effect of different drying methods used in the preparation protein concentrates from cultivars cowpea and bambara bean was investigated. Protein extracted by isoelectric precipitation method subsequently dried cabinet (CD), vacuum (VD) freeze (FD). extraction yield content were high. subunit composition characterized electrophoresis. affected water-holding capacity, oil absorption capacity solubility depending on cultivars. Water-holding adequate for its utilization viscous foods....

The effects of limited enzymatic hydrolysis with pepsin on the functional properties and molecular characteristics cowpea protein concentrate (CPC) were investigated. Hydrolysates prepared at degrees (DH) 5–30%. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis analysis demonstrated that globulin was major component CPC, breakdown polypeptides selective. As a result, significant changes in hydrolysates noticed as compared to concentrate. Then, (CPHs) showed good water solubility...

Secondary structure of protein, surface hydrophobicity, and functional properties defatted Moringa olifeira seed leaf flour were studied. Protein presented high β-sheet (32.77–41.11%) β-turn (37.97–39.31%) structures. Water holding oil absorption capacities adequate for using in food formulations. Surface hydrophobicity increased significantly at pH 9. Leaf protein was more soluble than the one, excepted 4–5. Neutral basic favoured emulsifying capacity (EC) flour. The maximum EC 52.17%....

This study was carried out to correlate grain legume consumption the prevention of type II diabetes and some cardiometabolic diseases (CMD) (high blood pressure, obesity, stroke). An exploratory survey among population (n = 468) Diamaré division (Far-north, Cameroon). In addition, nutritional properties selected legumes (GLs) were determined. Results showed generally that soybean, Bambara bean, cowpea linked metabolic within studied population. The these GLs also noteworthy. Remarkable...

Cowpea protein hydrolysates were prepared using thermolysin, alcalase, and trypsin analysed for bioactive properties, then, the release of peptides was investigated in silico. It found that degree hydrolysis reached 48% after 24 h with alcalase. The hydrolysate alcalase showed higher ACE inhibitory (62%) DPPH scavenging activity (19%). SDS-PAGE analysis revealed vignin major cowpea isolate. In silico indicated presence potential potent bioactivity primary structure proteins. 3D proteins...

Effect of physicochemical properties and secondary structure conformation, on the functional protein concentrates prepared from grain legumes was investigated using response surface methodology. Appropriate models were built basis best subset technique. Oil-holding capacity mainly influenced by proteins, particularly unordered structure, β-sheet, β-turn. Protein solubility depended hydrophobicity decreased with increasing hydrophobicity. Interaction between significant for emulsifying...

Cowpea is a source of low-cost and good nutritional quality protein for utilization in food formulations replacement animal proteins. Therefore it necessary that cowpea exhibits functionality, particularly solubility which affects the other functional properties. The objective this study was to produce hydrolysate exhibiting optimum by adequate combination hydrolysis parameters, namely time, solid/liquid ratio (SLR) enzyme/substrate (ESR), determine its properties molecular characteristics.A...

Abstract The Sudano-Sahelian zone of Cameroon is characterized by a hot tropical climate and poor sanitation, favorable to the proliferation synanthropic flies endemicity diarrheal diseases. Through their activities, proliferating can increase transmission diseases in region. This study aimed determine factors that influence variation diversity dynamics contribute control Monthly capture was carried out at 63 stratified randomly selected sites over nine months, covering both rainy dry...

The present study was undertaken to improve functional properties of cowpea protein concentrate by acylation and partial hydrolysis with pepsin. acylated showed significant improvement in solubility water index, at neutral pH. In addition, increased fat absorption capacity compared the untreated concentrate, maximum obtained 0.75 g succinic anhydride/g concentrate. Acetylation concentrations 0.25-0.50 g/g led higher emulsifying activity, a markedly stability observed 1.0 Foaming activity...

Mycosporine-like amino acids (MAAs) are natural UV-absorbing compounds found in microalgae and macroalgae. The content of MAAs algae varies with the seasons environmental factors. Red alga dulse Usujiri (Hokkaido, Japan) is an underutilized resource. Therefore, we investigated amount 2022 to clarify suitable months for MAA extraction. In addition, also evaluated extraction method focusing on volume. were prepared via 20 volumes 25% ethanol detected HPLC. results showed that 25 March highest...