A5065029431- Peptidase Inhibition and Analysis
- Enzyme Production and Characterization
- Protein Hydrolysis and Bioactive Peptides
- Chemical Synthesis and Analysis
- Carbohydrate Chemistry and Synthesis
- Biofuel production and bioconversion
- Protein Kinase Regulation and GTPase Signaling
- Enzyme Catalysis and Immobilization
- Studies on Chitinases and Chitosanases
- Microbial Natural Products and Biosynthesis
- Neuropeptides and Animal Physiology
- Biochemical effects in animals
- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- Plant Virus Research Studies
- Microbial Metabolites in Food Biotechnology
- Protease and Inhibitor Mechanisms
- Ferrocene Chemistry and Applications
- Ginger and Zingiberaceae research
- Genomics, phytochemicals, and oxidative stress
- RNA and protein synthesis mechanisms
- Polyamine Metabolism and Applications
- Drug-Induced Hepatotoxicity and Protection
- Biochemical and biochemical processes
- Influenza Virus Research Studies
Institute for Biological Sciences
2005-2022
The University of Tokyo
2021
Kobe University
2021
Hokkaido University
2021
Hokkaido University of Science
2021
Nissan Chemical Corporation (Japan)
2004-2006
Tokushima University
2000
Japan Advanced Institute of Science and Technology
2000
Kuraray (Japan)
1995-1996
Toshiba (Japan)
1996
The plant pathogen Ralstonia solanacearum uses a large repertoire of type III effector proteins to succeed in infection. To clarify the function host eukaryote cells, we expressed effectors yeast cells and identified seven that interfere with growth. One proteins, RipAY, was found share homology ChaC family as γ-glutamyl cyclotransferases, which degrade glutathione (GSH), tripeptide plays important roles immune system. RipAY significantly inhibited growth simultaneously induced rapid GSH...
The collagen tripeptide fragments Gly-Ala-Hyp, Gly-Pro-Ala and Gly-Pro-Hyp were generated by hydrolyzing from pig-skin, cattle-skin, fish-scales chicken-feet, respectively, with Streptomyces collagenase. Collagenase treatment increased the concentration of tripeptides in hydrolysates 13-15% (w/w). Of three peptides, was a true peptidic inhibitor dipeptidylpeptidase-IV (DPP-IV), because DPP-IV could not hydrolyze bond between Pro-Hyp. This moderately competitive (Ki=4.5 mM) DPP-IV, its level...
In this study, the α-glucosidase (maltase-glucoamylase: MGAM) and α-amylase inhibitory properties elicited by xylooligosaccharides (XOSs) prepared from dulse xylan were analysed as a potential mechanism to control postprandial hyperglycaemia for type-2 diabetes prevention treatment. Xylan was purified red alga powder used enzymatic hydrolysis using Sucrase X produce XOSs. Fractionation of XOSs produced xylobiose (X2), β-(1→3)-xylosyl (DX3), xylotriose (X3), β-(1→3)-xylosyl-xylotriose (DX4),...
Ferulic acid (FA), a component of hemicellulose in plant cell walls, is phenolic with several potential applications based on its antioxidant properties. Recent studies have shown that feruloyl esterase (FAE) key bacterial enzyme involved FA production from agricultural biomass. In this study, we screened library 43 esterases Streptomyces species and identified two enzymes, R18 R43, FAE activity toward ethyl ferulate. addition, characterized their properties detail. R43 showed other...
Endo-β-1,4-mannanases from Streptomyces thermolilacinus (StMan) and Thermobifida fusca (TfMan) demonstrated different substrate specificities. StMan hydrolyzed galactosylmannooligosaccharide (GGM5; 6(III) ,6(IV) -α-d-galactosyl mannopentaose) to GGM3 M2, whereas TfMan GGM5 GGM4 M1. To determine the region involved in specificity, we constructed chimeric enzymes of evaluated their Moreover, crystal structure catalytic domain (StMandC) complex inactive mutant StE273AdC with M6 were solved at...
ABSTRACT Feruloyl esterases (FAEs) are key enzymes required for the production of ferulic acid from agricultural biomass. Previously, we identified and characterized R18, an FAE Streptomyces cinnamoneus NBRC 12852, which showed no sequence similarity to known FAEs. To determine region involved in its catalytic activity, constructed chimeric using R18 homolog (TH2-18) S. strain TH-2. Although TH2-18 74% identity their primary sequences, recombinant proteins these two FAEs (recombinant [rR18]...
We previously showed that commercially available rice peptide Oryza Peptide-P60 (OP60) increased the intracellular glutathione levels. This study aimed to evaluate antioxidant potential of this and assess its mechanism action. Pretreatment HepG2 cells with OP60 reduced cytotoxicity caused by H2O2 or acetaminophen (APAP) (47.7 ± 1.3% 12.2 for 5 mg/mL pretreatment compared in H2O2- APAP-treated groups, respectively; p < 0.01) through restoration homeostasis. Moreover, elevated mRNA level genes...
Journal Article Purification and Characterization of Poly(vinyl alcohol) Dehydrogenase from Pseudomonas sp. 113P3 Get access Tadashi Hatanaka, Hatanaka Kuraray Co., Ltd., 2045-1 Sakazu, Kurashiki, Okayama 710, Japan Search for other works by this author on: Oxford Academic Google Scholar Noriko Asahi, Asahi Masao Tsuji Bioscience, Biotechnology, Biochemistry, Volume 59, Issue 10, 1 January 1995, Pages 1813–1816, https://doi.org/10.1271/bbb.59.1813 Published: 01 1995 history Received: 16 November 1994
Glutathione, the most abundant intracellular antioxidant, protects cells against reactive oxygen species induced oxidative stress and regulates redox status. We found that rice peptides increased glutathione levels in human hepatoblastoma HepG2 cells. Acetaminophen is a commonly used analgesic. However, an overdose of acetaminophen causes severe hepatotoxicity via depletion hepatic glutathione. Here, we investigated protective effects on acetaminophen-induced mice. ICR mice were orally...
ABSTRACT Dipeptide synthesis by aminopeptidase from Streptomyces septatus TH-2 (SSAP) was demonstrated using free amino acid as an acyl donor and aminoacyl methyl ester acceptor in 98% methanol (MeOH). SSAP retained its activity after more than 100 h MeOH, the case of phenylalanyl-phenylalanine synthesis, enzyme reaction reached equilibrium when 50% phenylalanine converted to product. In investigation specificity toward donors acceptors, showed a broad various acids esters. Furthermore, we...
The reducing tetrasaccharide TMG-chitotriomycin (1) is an inhibitor of β-N-acetylglucosaminidase (GlcNAcase), produced by the actinomycete Streptomyces anulatusNBRC13369. shows a unique inhibitory spectrum, that is, selectivity toward enzymes from chitin-containing organisms such as insects and fungi. Nevertheless, its structure-selectivity relationship remains to be clarified. In this study, we conducted structure-guided search analogues 1 in order obtain diverse...