A5088436888- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Asthma and respiratory diseases
- vaccines and immunoinformatics approaches
- Contact Dermatitis and Allergies
- Antimicrobial Peptides and Activities
- Pineapple and bromelain studies
- Transgenic Plants and Applications
- Antifungal resistance and susceptibility
- Metal-Catalyzed Oxygenation Mechanisms
- Toxin Mechanisms and Immunotoxins
- Machine Learning in Bioinformatics
- Mass Spectrometry Techniques and Applications
- Computational Drug Discovery Methods
- Herbal Medicine Research Studies
- Analytical Chemistry and Chromatography
- Advanced Proteomics Techniques and Applications
- Papaya Research and Applications
- Signaling Pathways in Disease
- Studies on Chitinases and Chitosanases
- Indoor Air Quality and Microbial Exposure
- Environmental Toxicology and Ecotoxicology
- Plant Stress Responses and Tolerance
- Advanced Chemical Sensor Technologies
Visva-Bharati University
2019-2024
Presidency University
2023
Bose Institute
2012-2020
Coconut pollen, one of the major palm pollen grains is an important constituent among vectors inhalant allergens in India and a sensitizer for respiratory allergy susceptible patients. To gain insight into its allergenic components, proteins were analyzed by two-dimensional electrophoresis, immunoblotted with coconut sensitive patient sera, followed mass spectrometry IgE reactive proteins. being largely unsequenced, proteomic workflow has been devised that combines conventional...
Background Respiratory allergy triggered by pollen allergens is increasing at an alarming rate worldwide. Sunflower thought to be important source of inhalant allergens. Present study aims identify the prevalence sunflower pollinosis among Indian allergic population and characterizes using immuno-proteomic tools. Methodology Clinico-immunological tests were performed understand sensitivity towards atopic population. Sera from selected positive patients used as probe detect IgE-reactive...
Background Fungal allergy is considered as serious health problem worldwide and increasing at an alarming rate in the industrialized areas. Rhizopus oyzae a ubiquitously present airborne pathogenic mold important source of inhalant allergens for atopic population India. Here, we report biochemical immunological features its 44 kDa sero-reactive aspartic protease allergen, which given official designation 'Rhi o 1'. Method The natural Rhi 1 was purified by sequential column chromatography...
Papaya has been reported to elicit IgE-mediated hypersensitivity via pollen inhalation and fruit consumption. Certain papaya sensitive patients with food allergy were found experience recurrent respiratory distresses even after quitting the consumption of fruits. This observation prompted us investigate allergens commonly present in fruits grains papaya. A discovery approach consisting immunoproteomic detection followed by molecular characterization led identification a novel allergen...
Cyclophilins are structurally conserved pan-allergens showing extensive cross-reactivity. So far, no precise information on cross-reactive IgE-epitopes of cyclophilins is available. Here, an 18-kDa IgE-reactive cyclophilin (Rhi o 2) was purified from Rhizopus oryzae, indoor mold causing allergic sensitization. Based LC-MS/MS-derived sequences natural Rhi 2, the full-length cDNA cloned, and expressed as recombinant (r) allergen. Purified rRhi 2 displayed IgE-reactivity basophil degranulation...
Sunflower pollen was reported to contain respiratory allergens responsible for occupational allergy and pollinosis. The present study describes the comprehensive characterization of a major sunflower allergen Hel 6. Natural 6 purified from by anion exchange gel filtration chromatography. reacted with IgE-antibodies 57% 39 sunflower-sensitized patient sera suggesting it be allergen. patients were Indian origin suffering pollinosis allergic rhinitis. exhibited allergenic activity stimulating...
Abstract Superoxide dismutases are important group of antioxidant metallozyme and play role in ROS homeostasis salinity stress. The present study reports the biochemical properties a salt-tolerant Cu, Zn-superoxide from Avicennia marina ( Am _SOD). _SOD was purified leaf identified by mass-spectrometry. Recombinant cDNA bacterially expressed as homodimeric protein. Enzyme kinetics revealed high substrate affinity specific activity compared to many earlier reported SODs. An electronic...
Allergy is a type of abnormal immune reactions, which triggered by environmental antigens or allergens and mediated IgE antibodies. Now-a-days mass spectrometry the method choice for allergen identification based on homology searching. Here, we provide dataset associated with our previously published research article sunflower pollen (Ghosh et al., 2015 [1]). In this study allergenicity (Helianthus annuus) grains were primarily investigated clinical studies followed detailed...
Background Airborne fungi, a significant constituent of atmospheric bioaerosol, are well-known source allergens and can cause allergic rhinitis bronchial asthma in sensitive subjects. Rhizopus stolonifer, the filamentous fungus is widely distributed thread-like Mucoralean mold. Commonly found on bread surfaces, it takes food nutrients from causes damage to surface where lives. Although exposure risk assumable indoor environment, role this provoking symptoms pre-sensitized individuals,...
Next-generation allergy vaccines refer to allergen-derived attenuated molecules that can boost allergen-blocking IgG response. These antibodies are specifically directed toward the IgE epitope of allergens and interfere in allergen-IgE interaction. Our study is a computational approach design such against four widespread pan-allergens families. Pan-allergens display extensive immunological cross-reactivity due presence conserved T cell epitope. In this study, vaccine based on hapten-carrier...