A5091316546- Sphingolipid Metabolism and Signaling
- Lipid Membrane Structure and Behavior
- Protein Kinase Regulation and GTPase Signaling
- HIV/AIDS drug development and treatment
- Glycosylation and Glycoproteins Research
- Click Chemistry and Applications
- Cancer Genomics and Diagnostics
- Enzyme function and inhibition
- Cellular transport and secretion
- Galectins and Cancer Biology
- Endoplasmic Reticulum Stress and Disease
- Synthesis and Characterization of Heterocyclic Compounds
- Carbohydrate Chemistry and Synthesis
- Chromosomal and Genetic Variations
- Malaria Research and Control
- Monoclonal and Polyclonal Antibodies Research
- Supramolecular Self-Assembly in Materials
- Advanced Biosensing Techniques and Applications
- Renal Transplantation Outcomes and Treatments
- Biochemical and Molecular Research
- Blood groups and transfusion
- Advanced biosensing and bioanalysis techniques
- Peptidase Inhibition and Analysis
- Evolution and Genetic Dynamics
- Synthesis and biological activity
Virginia Tech
2015-2024
National Cancer Institute
2020-2022
Frederick National Laboratory for Cancer Research
2022
Center for Cancer Research
2022
National Institutes of Health
2020
University of Virginia
2015
Adenosine Therapeutics (United States)
2015
Sphingosine 1-phosphate (S1P) levels are significantly higher in blood and lymph than tissues. This S1P concentration difference is necessary for proper lymphocyte egress from secondary lymphoid tissue to maintain endothelial barrier integrity. Studies with mice lacking either sphingosine kinase (SphK) type 1 2 indicate that these enzymes the sole biosynthetic source of S1P, but they play different roles setting levels. We have developed a set drug-like SphK inhibitors, differing selectivity...
The two isoforms of sphingosine kinase (SphK1 and SphK2) are the only enzymes that phosphorylate to sphingosine-1-phosphate (S1P), which is a pleiotropic lipid mediator involved in broad range cellular processes including migration, proliferation, inflammation. SphKs targets for various diseases such as cancer, fibrosis, Alzheimer's sickle cell disease. Herein, we disclose structure–activity profile naphthalene-containing SphK inhibitors molecular modeling studies reveal key switch controls...
Glycosylation is a vital post-translational modification involved in range of biological processes including protein folding, signaling, and cell-cell interactions. In 2011, new type O-linked glycosylation was discovered, wherein the side-chain oxygen tyrosine modified with GalNAc residue (GalNAc-Tyr). At present, very little known about GalNAc-Tyr prevalence, function, or biosynthesis. Herein, we describe design synthesis GalNAc-Tyr-derived hapten its use generating selective monoclonal...
ABSTRACT Changes in the copy number of large genomic regions, termed variations (CNVs), contribute to important phenotypes many organisms. CNVs are readily identified using conventional approaches when present a fraction cell population. However, that only few genomes across population often overlooked but important; if beneficial under specific conditions, de novo CNV arises single genome can expand during selection create larger cells with novel characteristics. While reach methods study...
Extrachromosomal (ec) DNAs are genetic elements that exist separately from the genome. Since ecDNA can carry beneficial genes, they a powerful adaptive mechanism in cancers and many pathogens. For first time, we report contributing to antimalarial resistance Plasmodium falciparum, most virulent human malaria parasite. Using pulse field gel electrophoresis combined with PCR-based copy number analysis, detected two differ migration structure. Entrapment well low susceptibility exonucleases...
Biological conjugation is an important tool employed for many basic research and clinical applications. While useful, common methods of biological suffer from a variety limitations, such as (a) requiring the presence specific surface-exposed residues, lysines or cysteines, (b) reducing protein activity, and/or (c) stability solubility. Use photoreactive moieties including diazirines, azides, benzophenones provide alternative, mild approach to conjugation. Upon irradiation with UV visible...
Increases in the copy number of large genomic regions, termed genome amplification, are an important adaptive strategy for malaria parasites. Numerous amplifications across