A5071877501- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- Ion Channels and Receptors
- HER2/EGFR in Cancer Research
- Microtubule and mitosis dynamics
- 3D Printing in Biomedical Research
- Ion channel regulation and function
- Erythrocyte Function and Pathophysiology
- Glioma Diagnosis and Treatment
- Cell Adhesion Molecules Research
- Neurobiology and Insect Physiology Research
- Caveolin-1 and cellular processes
- Biosimilars and Bioanalytical Methods
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Magnesium in Health and Disease
- Cancer Genomics and Diagnostics
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related Molecular Pathways
- Lung Cancer Treatments and Mutations
- Pluripotent Stem Cells Research
Johns Hopkins University
2015-2025
Cells adopt distinct signaling pathways to optimize cell locomotion in different physical microenvironments. However, the underlying mechanism that enables cells sense and respond confinement is unknown. Using microfabricated devices substrate-printing methods along with FRET-based biosensors, we report that, as transition from unconfined confined spaces, intracellular Ca2+ level increased, leading phosphodiesterase 1 (PDE1)-dependent suppression of PKA activity. This elevation requires...
Tumor cell intravasation preferentially occurs in regions of low fluid shear because high is detrimental to tumor cells. Here, we describe a molecular mechanism by which cells avoid during intravasation. The transition from migration was modeled using microfluidic device where migrating inside longitudinal tissue-like microchannels encounter an orthogonal channel flow induces physiological stresses. This approach complemented with intravital microscopy, patch-clamp, and signal transduction...
Significance The discovery of human epidermal growth factor receptor 2 ( HER2 ) missense mutations in breast and other cancers potentially make such tumors susceptible to current future HER2-targeted therapies. However, the majority occur nonamplified cancers, whether these will predict for sensitivity HER2-directed therapies remains unknown. Using genome editing, data presented here suggest that are functionally distinct require additional oncogenic input impart cancerous phenotypes. These...
TRPM7 senses hydraulic pressure and modulates cortical actomyosin to guide migrating cells towards the path of lower resistance.
Cells tune adherens junction dynamics to regulate epithelial integrity in diverse (patho)physiological processes, including cancer metastasis. We hypothesized that the spatially confining architecture of peritumor stroma promotes metastatic cell dissemination by remodeling cell-cell adhesive interactions. By combining microfluidics with live-cell imaging, FLIM/FRET biosensors, and optogenetic tools, we show confinement induces leader dissociation from cohesive ensembles. Cell is triggered...