A5074547096- Pluripotent Stem Cells Research
- Developmental Biology and Gene Regulation
- Tissue Engineering and Regenerative Medicine
- melanin and skin pigmentation
- Skin Protection and Aging
- Neurogenesis and neuroplasticity mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Congenital heart defects research
- 3D Printing in Biomedical Research
- Mesenchymal stem cell research
- Genomics and Chromatin Dynamics
- Cancer Genomics and Diagnostics
- Cellular Mechanics and Interactions
- Single-cell and spatial transcriptomics
- Cell Adhesion Molecules Research
- Crystallography and molecular interactions
- Cancer Cells and Metastasis
- Skin and Cellular Biology Research
- Plant Gene Expression Analysis
- CRISPR and Genetic Engineering
- Chemical Reaction Mechanisms
- Renal and related cancers
- Hedgehog Signaling Pathway Studies
- Biosimilars and Bioanalytical Methods
- Cell Image Analysis Techniques
Stanford University
2019-2025
University of Oklahoma
2023
California Institute for Regenerative Medicine
2021
Stanford Medicine
2019
University at Buffalo, State University of New York
2011-2018
Buffalo State University
2014
State University of New York
2012
Indian Institute of Technology Kanpur
2008
Smooth muscle cells (SMC) play an important role in vascular homeostasis and disease. Although adult mesenchymal stem (MSC) have been used as a source of contractile SMC, they suffer from limited proliferation potential culture senescence, particularly when originating older donors. By comparison, human induced pluripotent (hiPSC) can provide unlimited functional SMC for autologous cell-based therapies creating models Our goal was to develop efficient strategy derive functional, hiPSC. We...
Skin color, one of the most diverse human traits, is determined by quantity, type, and distribution melanin. In this study, we leveraged light-scattering properties melanin to conduct a genome-wide screen for regulators melanogenesis. We identified 169 functionally genes that converge on melanosome biogenesis, endosomal transport, gene regulation, which 135 represented previously unknown associations with pigmentation. agreement their melanin-promoting function, majority hits were...
Cellular senescence as a result of organismal aging or progeroid diseases leads to stem cell pool exhaustion hindering tissue regeneration and contributing the progression age related disorders. Here we discovered that ectopic expression pluripotent factor NANOG in senescent myogenic progenitors reversed cellular restored completely ability generate contractile force. To elicit its effects, enabled reactivation ROCK Transforming Growth Factor (TGF)-β pathways-both which were impaired...
Abstract Neural crest (NC) cells are multipotent stem that arise from the embryonic ectoderm, delaminate neural tube in early vertebrate development and migrate throughout developing embryo, where they differentiate into various cell lineages. Here we show functional NC can be derived by induction with a growth factor cocktail containing FGF2 IGF1 cultures of human inter-follicular keratinocytes (KC) isolated elderly donors. Adult exhibited longer doubling times as compared to neonatal...
During development, neural crest (NC) cells are induced by signaling events at the plate border of all vertebrate embryos. Initially arising within central nervous system, NC subsequently undergo an epithelial to mesenchymal transition migrate into periphery, where they differentiate diverse cell types. Here we provide evidence that postnatal human epidermal keratinocytes (KC), in response fibroblast growth factor 2 and insulin like 1 signals, can be reprogrammed toward a fate. Genome-wide...
The interaction between different cations and certain aromatic dimers (benzene, naphthalene, anthracene) heteroaromatic (pyridine, pyrazine, sym-triazine sym-tetrazine) has been investigated employing the MP2 level of theory with 6-31G** 6-311++G** basis sets. trend in stabilization energy for is found to be: (anthracene)2-cation>(naphthalene)2-cation>(benzene)2-cation. In all three cases, cation remains sandwiched rings. case systems, on other hand, prefers interact nitrogen atom ring....
Abstract Neural crest (NC) cells play a central role in forming the peripheral nervous system, craniofacial skeleton, and pigmentation of skin during development due to their broad multilineage differentiation potential into neurons, Schwann cells, melanocytes, mesenchymal stem cells. Recently, we identified an easily accessible source pluripotent NC from human inter‐follicular keratinocyte (KC) cultures (KC‐NC). In this work, examined specific conditions for derivation KC cultures. More...
Summary Through the production of chimeric animals, induced pluripotent stem cells (iPSCs) can generate personalized organs with diverse applications for both basic research and translational medicine. This concept was first validated in rodents by forming a rat pancreas mice vice versa. However, potential use human iPSCs to xenogenic other species is technically ethically difficult. Recognizing these concerns, we explored generation nonhuman primates (NHP) embryos, injecting either...
Abstract The skin color is one of the most diverse human traits and determined by quantity, type distribution melanin. Here, we leverage light scattering properties melanin to conduct a genome-wide CRISPR-Cas9 screen for novel regulators melanogenesis. We identify functionally genes converging on melanosome biogenesis, endosomal transport transcriptional/posttranscriptional gene regulation, which represent associations with pigmentation. A survey transcriptomes from diversely pigmented...