A5054930802- Venous Thromboembolism Diagnosis and Management
- Pulmonary Hypertension Research and Treatments
- Tuberculosis Research and Epidemiology
- Blood Coagulation and Thrombosis Mechanisms
- Blood properties and coagulation
- Acute Myocardial Infarction Research
- Atrial Fibrillation Management and Outcomes
- Cardiovascular Conditions and Treatments
- Mycobacterium research and diagnosis
- Pneumonia and Respiratory Infections
- Cardiac tumors and thrombi
- Innovative Microfluidic and Catalytic Techniques Innovation
- HIV, Drug Use, Sexual Risk
- Biosensors and Analytical Detection
- Cardiovascular Issues in Pregnancy
- Nitric Oxide and Endothelin Effects
- Cardiovascular Effects of Exercise
- Pneumothorax, Barotrauma, Emphysema
- Erythrocyte Function and Pathophysiology
- Platelet Disorders and Treatments
- Pleural and Pulmonary Diseases
- Cancer therapeutics and mechanisms
- Hemophilia Treatment and Research
- Transplantation: Methods and Outcomes
- Pneumocystis jirovecii pneumonia detection and treatment
University of California, San Diego
2006-2023
University of California System
1997-2019
UC San Diego Health System
2017
In-Q-Tel
2004
GTx (United States)
2004
American Lung Association
2004
Although acute pulmonary embolism is epidemiologically associated with chronic thromboembolic hypertension, the factors responsible for resistance to thrombolysis and a shift toward vascular remodeling within arteries of patients hypertension are unknown.Determine whether fibrin from more resistant plasmin-mediated lysis than healthy control subjects.Fibrinogen purified subjects was used prepare clots, which were subsequently digested plasmin various periods time. The degradation alpha-,...
It is well known that endothelin (ET)-1 mediates vascular remodelling in various kinds of clinical and experimental pulmonary hypertension. The aim this study was to investigate whether ET-1 associated with the development a canine model chronic embolic Pulmonary hypertension induced 10 mongrel dogs by repeated embolization ceramic beads. In five dogs, bosentan, nonselective ET receptor antagonist, administered throughout study. Haemodynamic measurements plasma assays were performed every 2...
We determined the amide hydrogen/deuterium exchange profile of native human fibrinogen under physiologic conditions. After optimization quench and proteolysis conditions, more than 1,200 peptides were identified by mass spectrometry, spanning 90% constituent Aα, Bβ, γ chain amino acid sequences. The compact central distal globular regions well protected from deuterium exchange, with exception unfolded amino-terminal segments Aα Bβ chains extending region, short "tail" each region....
BACKGROUND The brisk fibrinolytic response of canines has impaired efforts to develop a canine model chronic thromboembolic pulmonary hypertension. Difficulties in retaining embolic residuals were partially overcome by administration tranexamic acid (TXA) (Circulation. 1991;83:1272-1279.). In this study, we used type 1 plasminogen activator inhibitor (PAI-1), major the endogenous system, determine its efficacy suppression thrombolysis canines. METHODS AND RESULTS Thrombus was induced...
Previous attempts to diagnose thromboemboli using radiolabeled antibodies and nuclear medicine imaging have been disappointing. We present the results of experiments with intravenous technetium-99m-labeled deimmunized antifibrin Fab' fragments single photon emission computed tomography (SPECT), a highly sensitive scintigraphic technique. Pulmonary emboli (PEs) lower extremity deep vein thrombi (DVTs) were formed in five dogs, then ( approximately 400 mg, 260 MBq) injected via forelimb veins....
Acute pulmonary embolism occurs in more than half a million people year the United States. Chronic thromboembolic hypertension (CTEPH) develops ∼4% of these patients due to unresolved thromboemboli. CTEPH is thus relatively common, progressive, and potentially fatal disease. One currently proposed theory for poor resolution advocates that modification fibrinogen causes resistance emboli fibrinolysis. The current study investigated regulation cytosolic Ca 2+ ([Ca ] cyt ), central control cell...
Residual pulmonary vascular obstruction (RPVO) and chronic thromboembolic hypertension (CTEPH) are both long-term complications of acute embolism, but it is unknown whether RPVO can be predicted by variants fibrinogen associated with CTEPH. We used the Akaike information criterion to select best predictive models for in two prospectively followed cohorts embolism patients, using as candidate variables extent initial obstruction, clinical characteristics fibrinogen-related data. measured...
While the goal of universal drug susceptibility testing has been a key component WHO End TB Strategy, in practice, this remains inaccessible to many. Rapid molecular tests for tuberculosis (TB) and antituberculosis resistance could significantly improve access testing. In study, we evaluated accuracy Akonni Biosystems XDR-TB (extensively drug-resistant TB) TruArray lateral-flow-cell (XDR-LFC) assay (Akonni Biosystems, Inc., Frederick, MD, USA), novel that detects mutations seven genes...
Background This study was performed to determine whether antibodies against the amino-terminus of β-chain fibrin (anti-β) could noninvasively distinguish actively enlarging thrombi from stabilized with anticoagulants. Methods and Results Dogs unilateral femoral vein were allocated into three groups: (1) no anticoagulation, (2) intravenous heparin maintained in “therapeutic” range (0.2 0.5 U/mL plasma), (3) “excess” heparin, at >1.0 plasma. Thrombolysis suppressed tranexamic acid. 111...
Persons who inject drugs (PWID) might be at increased risk for Mycobacterium tuberculosis infection and reactivation of latent tuberculous (LTBI) due to their injection drug use.To determine prevalence correlates M. among PWID in San Diego, California, USA.PWID aged 18 years underwent standardized interviews serologic testing using an interferon-gamma release assay (IGRA) LTBI rapid point-of-care assays human immunodeficiency virus (HIV) hepatitis C (HCV) infections. Independent were...
Chronic obstructive pulmonary disease (COPD) is a frequent source of hospitalization. Antibiotics are largely prescribed during COPD exacerbation. Our hypothesis that large broad-spectrum antibiotics more and frequently prescribed. results confirm this trend highlight the increase in use exacerbation unexplained.Acute (AECOPD) due to respiratory tract infection, benefit antipseudomonal (APA) still debated. Health care-associated pneumonia (HCAP) was defined 2005 requires antibiotherapy. The...
Radiolabelled anti-fibrin antibodies have not yet enabled reliable and practical diagnosis of venous thromboembolism. However, previous unsuccessful clinical trials were performed with beta-chain that do optimally bind to thrombi during anticoagulation. The current experiments determine if radiolabelled anti-D-dimer more reliably allowed nuclear medicine imaging deep anticoagulation than anti-beta-chain antibodies.Dogs pre-existing unilateral femoral vein anticoagulated heparin (300 units.kg...
The role of active thrombosis in the pathophysiology pulmonary embolism is unclear. We tested hypothesis that venous thrombi significantly increase their thrombotic activity once they embolize into high-flow circulation arteries. Thrombotic was measured using an immunoassay measures both fibrinopeptide B (FPB) as well its most abundant metabolite des-arginine FPB. Thrombi were formed femoral veins adult dogs. In one group, embolized without anticoagulation. second heparin (300 U/kg bolus,...
Despite the WHO's call for universal drug susceptibility testing all patients being evaluated tuberculosis (TB), a lack of rapid diagnostic tests which can fully describe TB resistance patterns is major challenge in ensuring that persons diagnosed with drug-resistant are started on an appropriate treatment regime. We accuracy Akonni Biosystems XDR-TB TruArray and lateral-flow cell (XDR-LFC), novel multiplex assay to simultaneously detect mutations across seven genes confer both first-...
The lack of accurate and efficient diagnostic devices for extensively drug-resistant tuberculosis (XDR-TB) makes it a severe threat to global public health. A prospective clinical study in an intended-use cohort was designed evaluate the Akonni Biosystems XDR-TB TruArray lateral flow cell (XDR-LFC) address this gap diagnostics.