A5073019213- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Diagnosis and treatment of tuberculosis
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced biosensing and bioanalysis techniques
- Infectious Diseases and Tuberculosis
- Respiratory viral infections research
- Metal complexes synthesis and properties
- Molecular Biology Techniques and Applications
- DNA and Nucleic Acid Chemistry
- Biosensors and Analytical Detection
- Pneumonia and Respiratory Infections
- Bacteriophages and microbial interactions
- Influenza Virus Research Studies
- SARS-CoV-2 detection and testing
- Prenatal Screening and Diagnostics
- Viral gastroenteritis research and epidemiology
- Tumors and Oncological Cases
- Parvovirus B19 Infection Studies
- CRISPR and Genetic Engineering
- Photochromic and Fluorescence Chemistry
- Bacterial Identification and Susceptibility Testing
- Microbial infections and disease research
- Molecular Sensors and Ion Detection
- Cyclization and Aryne Chemistry
Akonni Biosystems (United States)
2011-2024
EsSALUD
2020
Frederick Community College
2019
Scripps Research Institute
2005
University of North Carolina at Chapel Hill
1999-2004
Duke University
2003
Indiana University Bloomington
2003
Metal-to-ligand charge-transfer (MLCT) photolyses (lambda > or = 395 nm) of copper complexes cis-1,8-bis(pyridin-3-oxy)oct-4-ene-2,6-diyne (bpod, 1), [Cu(bpod)(2)]PF(6) (2), and [Cu(bpod)(2)](NO(3))(2) (3) yield Bergman cyclization the bound ligands. In contrast, uncomplexed ligand 1 Zn(bpod)(2)(CH(3)COO)(2) compound (4) are photochemically inert under same conditions. case 4, sensitized photochemical generation lowest energy (3)pi-pi state, which is localized on enediyne unit, leads to...
We examined Mycobacterium tuberculosis DNA detection from buccal swab samples collected children in Lima, Peru. was extracted and amplified via real-time polymerase chain reaction. Sensitivity 21% (95% confidence interval [CI]: 7%–42%) 24 culture-confirmed cases 4.6% CI: 1%–13%) 65 clinically diagnosed unconfirmed cases. highest for smear-positive tuberculosis. Specificity 99% the 199 controls 96%–100%).
The electrocatalytic oxidation of guanine in DNA and oligonucleotides by Ru(bpy)33+/2+ was investigated using cyclic voltammetry (CV) chronoamperometry (CA) (bpy = 2,2'-bipyridine). Oxidation Ru(bpy)32+ to the Ru(III) form at tin-doped indium oxide (ITO) electrodes presence produces catalytic current due Ru(III). CA traces with calf thymus high salt concentration (50 mM sodium phosphate + 700 NaCl) give a single kinetic process rate constant 3500 ± 300 M-1 s-1 that is independent similar...
Rapid and accurate diagnosis of childhood tuberculosis (TB) is challenging because children are often unable to produce the sputum sample required for conventional tests. Stool an alternative type that easy collect from children, studies investigating use stool molecular detection Mycobacterium (Mtb) have led promising results. Our objective was evaluate as specimen Mtb in children. We did so using TruTip workstation (Akonni Biosystems), a novel automated lysis extraction platform. tested...
We describe a Lab-on-a-Film disposable that detects multidrug-resistant tuberculosis (MDR-TB) from sputum extracts. The consists of 203 gel elements include DNA sequences (probes) for 37 mutations, deletions, or insertion across 5 genes (including an internal control). These are printed on flexible film, which costs approximately 500 times less than microarray glass. film with is then laminated to additional rollable materials (films) form microfluidic flow cell. combined multiplex...
Due to the low percentage of fetal DNA present in maternal plasma (< 10%) during early gestation, efficient extraction processes are required for successful downstream detection applications non-invasive prenatal diagnostic testing. In this study, two methods using similar chemistries but different workflows were compared isolation efficiency and percent recovery. The Akonni Biosystems TruTip technology uses a binding matrix embedded pipette tip; Circulating Nucleic Acids Kit from Qiagen...
Abstract Tuberculosis (TB) diagnosis relies on a sputum sample, which cannot be easily obtained from all symptomatic patients. Mycobacterium tuberculosis DNA can detected oral swabs, noninvasive, safe alternative sample type; however, reported sensitivities have been variable and likely depend collection, processing procedures host characteristics. We analyzed three buccal swab samples 123 adults with culture-confirmed TB in Lima, Peru. compared the sensitivity specificity of two collection...
There is a growing awareness that molecular diagnostics for detect-to-treat applications will soon need highly multiplexed mutation detection and identification capability. In this study, we converted an open-amplicon microarray hybridization test multidrug-resistant (MDR) Mycobacterium tuberculosis into entirely closed-amplicon consumable (an amplification microarray) evaluated its performance with matched sputum sediment extracts. Reproducible genotyping (the limit of detection) was...
Automated genotyping of drug-resistant Mycobacterium tuberculosis (MTB) directly from sputum is challenging for three primary reasons. First, the sample matrix, sputum, highly viscous and heterogeneous, posing a challenge processing. Second, acid-fast MTB bacilli are difficult to lyse. And third, there hundreds mutations that confer drug resistance. An additional constraint most prevalent where test affordability paramount. We address homogenization cell lysis using magnetic rotation an...
While the goal of universal drug susceptibility testing has been a key component WHO End TB Strategy, in practice, this remains inaccessible to many. Rapid molecular tests for tuberculosis (TB) and antituberculosis resistance could significantly improve access testing. In study, we evaluated accuracy Akonni Biosystems XDR-TB (extensively drug-resistant TB) TruArray lateral-flow-cell (XDR-LFC) assay (Akonni Biosystems, Inc., Frederick, MD, USA), novel that detects mutations seven genes...
TruTip is a simple nucleic acid extraction technology whereby porous, monolithic binding matrix inserted into pipette tip. The geometry of the monolith can be adapted for specific tips ranging in volume from 1.0 to 5.0 ml. large porosity enables viscous or complex samples readily pass through it with minimal fluidic backpressure. Bi-directional flow maximizes residence time between and sample, sample volumes processed within single TruTip. fundamental steps, irrespective geometry, include...
Automated nucleic acid extraction from primary (raw) sputum continues to be a significant technical challenge for molecular diagnostics. In this work, we developed prototype open-architecture, automated workstation that includes mechanical homogenization and lysis function integrated with heating TruTip purification; optimized an protocol raw sputum; evaluated system performance on clinical specimens. Eight samples could processed within 70 min. The efficiently homogenized sputa doubled...
The electrocatalytic oxidation of DNA by a heterodimer, [(bpy)2Ru(tpphz)Os(bpy)2]4+ (tpphz: tetrapyrido[3,2-alpha: 2',3'-c:3'',2''-h:2''',3'''-j]phenazine) (1), was studied using cyclic voltammetry with digital simulation. This dimer chosen because the Ru(III/II) couple (E1/2 = 1.09 V vs Ag/AgCl) is capable catalyzing guanine while Os(III/II) 0.63 V) provides convenient reporter on binding and mass transport complex, which can then be determined in same voltammetric sweep as...
A phosphoramidite containing Os(bpy)32+ (Os; bpy, 2,2'-bipyridine) with a three-carbon linker was synthesized and used to prepare oligonucleotides the Os redox catalyst appended 5'-end. The electrogenerated Os(III) is capable of oxidizing 7,8-dihydro-8-oxo-guanine (8G), but 8G not electrochemically reactive at indium tin oxide electrodes because poor electrode kinetics for direct reaction. hairpin-forming oligonucleotide Os-5'-ATG TCA GAT TAG CAG GCC TGA CAT characterized by thermal...
TruTip is a simple nucleic acid extraction technology whereby porous, monolithic binding matrix inserted into pipette tip. The geometry of the monolith can be adapted for specific tips ranging in volume from 1.0 to 5.0 ml. large porosity enables viscous or complex samples readily pass through it with minimal fluidic backpressure. Bi-directional flow maximizes residence time between and sample, sample volumes processed within single TruTip. fundamental steps, irrespective geometry, include...
While there has been progress in detection of drug resistant tuberculosis globally, WHO estimates only about half the patients with bacteriologically confirmed were tested for rifampicin resistance over past two years. To close this diagnostic gap, an expansion testing and isoniazid is critically needed. The Akonni Biosystem Integrated System combines DNA extraction a Lab-on-a-Film assembly (LFA) to perform rapid probe PCR-based associated mutations first-line anti-tuberculosis drugs. Using...
Electrocatalytic oxidation of the oligonucleotide 5'- GAA GAG GTT TTT CCT CTT CTC C (TS) by Ru(bpy)32+ was studied cyclic voltammetry. This forms either an intramolecular triplex, hairpin, or single strand, depending on pH (Plum, G. E.; Breslauer, K. J. Mol. Biol. 1995, 248, 679−695). In triplex form, guanine doublet in TS is buried inside folded structure, and as such less susceptible to electrogenerated Ru(bpy)33+. Digital simulations catalytic voltammograms gave a rate constant 3.5 ± 0.2...