A5085882492- Lipid Membrane Structure and Behavior
- Sphingolipid Metabolism and Signaling
- Erythrocyte Function and Pathophysiology
- Lipid metabolism and biosynthesis
- Natural Products and Biological Research
- Metabolism and Genetic Disorders
- Plant and soil sciences
- DNA and Nucleic Acid Chemistry
- Acute Myeloid Leukemia Research
- Fatty Acid Research and Health
- Soil Science and Environmental Management
- Sugarcane Cultivation and Processing
- Pineapple and bromelain studies
- DNA Repair Mechanisms
- Advanced biosensing and bioanalysis techniques
- Cancer, Lipids, and Metabolism
- Cellular transport and secretion
- Metabolomics and Mass Spectrometry Studies
- Nanopore and Nanochannel Transport Studies
- Glycogen Storage Diseases and Myoclonus
- Developmental and Educational Neuropsychology
- ATP Synthase and ATPases Research
- Psidium guajava Extracts and Applications
- TGF-β signaling in diseases
- Philosophical Thought and Analysis
University of the Basque Country
2005-2021
Instituto Biofisika
2017-2021
Universitat de les Illes Balears
2010-2019
Colegio de Postgraduados
2005-2017
Research Institute of Health Sciences
2010-2015
Novartis (United States)
2013
Consejo Superior de Investigaciones Científicas
2012
Fundación Biofísica Bizkaia
2009
University of Colorado Denver
2008
University of Southern Denmark
2008
Nuclear receptors for 17β-estradiol (E2) are present in growth plate chondrocytes from both male and female rats regulation of through these has been studied many years; however, recent studies indicate that an alternative pathway involving a membrane receptor may also be involved the cell response. E2 was found to directly affect fluidity chondrocyte membranes derived female, but not male, rats. In addition, activates protein kinase C (PKC) nongenomic manner cells, chelerythrine, specific...
Membrane-mediated increases in protein kinase C (PKC) activity and PKC-dependent physiological responses of growth plate chondrocytes to vitamin D metabolites depend on the state endochondral maturation; 1α,25-dihydroxyvitamin D3 [1α,25-(OH)2D3] regulates zone (GC) cells, whereas 24R,25-(OH)2D3 resting (RC) cells. Different mechanisms, including A signaling, mediate effects 1α,25-(OH)2D3 PKC, suggesting that different mechanisms may also regulate any MAPK involvement responses. This study...
Hot−cold hemolysis is the phenomenon whereby red blood cells, preincubated at 37 °C in presence of certain agents, undergo rapid when transferred to 4 °C. The mechanism this not understood. PlcHR2, a phospholipase C/sphingomyelinase from Pseudomonas aeruginosa, that prototype new phosphatase superfamily, induces hot−cold hemolysis. We found sphingomyelinase, but C activity, essential for because occurs only human erythrocytes contain both phosphatidylcholine (PC) and sphingomyelin (SM) also...
Recent research regarding 2-hydroxylated fatty acids (2OHFAs) showed clear evidence of their benefits in the treatment cancer, inflammation, and neurodegenerative disorders such as Alzheimer's disease. Monolayer compressibility isotherms isothermal titration calorimetry 2OHFA (C18-C22) phosphatidylcholine/phosphatidylethanolamine/sphingomyelin/cholesterol (1:1:1:1 mole ratio), a mixture that mimics composition mammalian plasma membrane, were performed to assess membrane binding capacity...
Due to the low percentage of fetal DNA present in maternal plasma (< 10%) during early gestation, efficient extraction processes are required for successful downstream detection applications non-invasive prenatal diagnostic testing. In this study, two methods using similar chemistries but different workflows were compared isolation efficiency and percent recovery. The Akonni Biosystems TruTip technology uses a binding matrix embedded pipette tip; Circulating Nucleic Acids Kit from Qiagen...
The plasma membrane is an attractive target for new anticancer drugs, not least because regulating its lipid structure can control multiple signaling pathways involved in cancer cell proliferation, differentiation and survival. Accordingly, the novel drug hydroxytriolein (HTO) was designed to interact with regulate composition of membrane, which turn controls interaction amphitropic proteins bilayer. Changes provoked by HTO impair growth triple negative breast (TNBC) cells, aggressive tumor...
Membrane lipid therapy is a novel approach to rationally design or discover therapeutic molecules that target membrane lipids. This strategy has been used synthetic fatty acid analogs are currently under study in clinical trials for the treatment of cancer. In this context, and with aim controlling tumor cell growth, we have designed synthesized hydroxylated analog triolein, hydroxytriolein (HTO). Both triolein HTO regulate biophysical properties model membranes, they inhibit growth...
The binding and early stages of activity a phospholipase C/sphingomyelinase from Pseudomonas aeruginosa on giant unilamellar vesicles (GUV) have been monitored using fluorescence confocal microscopy. Both the lipids enzyme were labeled with specific fluorescent markers. GUV consisted mixture phosphatidylcholine, sphingomyelin, phosphatidylethanolamine, cholesterol in equimolar ratios, to which 5-10 mol% end-product ceramide and/or diacylglycerol occasionally added. Morphological examination...
In recent years much attention has focused on the impacts of agriculture climate change, due to this stage specialists in plant nutrition and soil fertility have achieved task generating adequate fertilization doses for pineapple. A methodology Integrated System Recommending Fertilizer Dose, was used. As a result, seven Thiessen polygons, where rainfall ranged from 1640 2841 mm correlated. Therefore, three major groups were defined classified as subunit level. Likewise, eight fertilizers...
Adult polyglucosan body disease (APBD) is a neurological disorder characterized by adult-onset neurogenic bladder, spasticity, weakness, and sensory loss. The caused aberrant glycogen branching enzyme (GBE) (GBE1Y329S) yielding less branched, globular, soluble glycogen, which tends to aggregate. We explore here whether, despite being enzyme, GBE1 activity regulated protein-membrane interactions. Because proteins can contact wide variety of cell membranes, we investigated the interactions...