Milk is considered as complete food and an important part of human diet throughout the world including India. Bacterial contamination milk such Escherichia coli due to unhygienic condition poor udder health can cause infections, especially in infants elders or immunocompromised persons. Possession antimicrobial resistance genes by commensal bacteria present makes issue more serious.The study was aimed isolate characterize extended-spectrum beta-lactamase (ESBL)-producing E. from samples...

Aim: Subclinical mastitis in bovines is mainly responsible for the huge economic loss of dairy farmers, which Pseudomonas aeruginosa one causative agents.The study was aimed at a screening suspected milk samples from different cattle farms West Bengal detection and confirmation P. strains followed by their characterization. Materials Methods:Around 422 were screened primarily on-spot bromothymol blue (BTB) test then lab somatic cell counts (SCC) to finally consider 352 aeruginosa.Selective...

Background: Salmonella spp. is a pathogenic bacterium that can cause infection in animals and human beings. Drug-resistant Salmonellae make this more deadly. In background, study was undertaken to investigate the prevalence antimicrobial resistance pattern of ESBL / ACBL-producing enterica strains, obtained from bovine faecal samples different districts West Bengal. Methods: A total 170 were aseptically collected Purba Barddhaman, Birbhum North 24 Paraganas study. Standard procedures...

Aim:The ringworms of pet dogs, cats, and stray animals (dogs, other animals) could be a potential source zoonotic infections causing serious public health problem in the busy city Kolkata.The owners are more susceptible to get this infection from their pets, because close contact with them as dermatophytosis is very much prevalent those pets.So, study was aimed check prevalence owners.Materials Methods: A total 362 clinically suspected cases dogs (123 number), cats (202 human beings (37...

The aim was to characterize Salmonella enterica serovar Gallinarum isolated from backyard poultry by polymerase chain reaction (PCR) detection of virulence genes invasion (invA) and plasmid C (spvC).Two strains isolates used in this study were obtained an outbreak fowl typhoid Vanaraja fowl. PCR technique for invA spvC using standard methodology. product one representative isolate sequenced compared with other related serovars GenBank data.Salmonella produced expected amplicons gene...

Escherichia coli is quite a common and sometimes pathogenic bacterium that can cause acute to severe infection in poultry birds affecting their performance.E. also few extra-intestinal infections too birds.Nowadays antimicrobial resistance menace which reported from several gram-negative bacteria including E. coli.These traits are governed by β β-lactamases producing genes may be associated with plasmids the pathogen.This study aimed detect presence of extended-spectrum (ESBL) AmpC (ACBL)...

This study was undertaken to detect the occurrence of beta-lactamase and biofilm producing Enterobacteriaceae in healthy ducks. A total 202 cloacal swabs were collected from ducks kept organized (n = 92) backyard 110) farms West Bengal (India). The had no history antibiotic intake. Among 87 phenotypically Escherichia coli, 19 (17·43%), 6 (5·05%) 15 (13·76%) isolates possessed blaTEM , blaSHV blaCTX-M respectively. Whereas, 5 (38·46%) Salmonella found harbour . In K. pneumoniae 10 (33·33%), 3...

Antimicrobial resistance is a global emergency which needs one health approach to address. The present study was conducted detect the prevalence of beta-lactamase and biofilm-producing Klebsiella strains in rectal swabs (n = 624) collected from healthy dogs, cats, sheep goats reared as companion or household animals India. dogs cats were frequently exposed third- fourth-generation cephalosporins for therapy. occasionally antibiotics had environmental exposure. Phenotypical ESBL 93) ACBL...

This study was carried out to assess the presence of anti-bluetongue (BT) antibodies in sheep, goat and cattle different agro-climatic zones Jharkhand.Serum samples were collected from apparently healthy as well suspected districts Jharkhand covering zones. Serum screened by indirect enzyme linked immunosorbent assay (iELISA) for detecting anti-BT antibodies.Out a total 480 animal serum (sheep-190, goats-210 cattle-80) screened, 83 (43.68%) 91 (43.33%) 46 (57.50%) sera found positive. The %...

the present study was undertaken to detect incidence of extended-spectrum-cephalosporinase gene possessing-Escherichia coli, its co-resistance pattern against other antimicrobials, and clonal relationship isolates in healthy kuroiler birds.a total 80 cloacal swabs from kuroilers were collected randomly West Bengal, India.the use costly antimicrobials (cephalosporins) not practiced by farmers.Escherichia coli isolated identified standard biochemical tests 16SrRNA-PCR.All E. isolates,...

In the current study prevalence and characterization of extended spectrum beta-lactamase(ESBL) production Escherichia coli was detected from broilers West Bengal, India. A total 248 cloacal swabs samples were examined for E.coli by standard bacteriological techniques. isolates serotyped also screened beta-lactamase double disc diffusion assay PCR detection blaCTX-M, blaSHV, blaTEM genes. The antimicrobial sensitivity profile isolated demonstrated against 13 agents. One hundred thirty one...

Fatty liver haemorrhagic syndrome (FLHS) in poultry is a non-infectious metabolic disease of unknown aetiology. An outbreak fatty was detected at post mortem from 42-week old caged Vencobb broiler breeders commercial farm (n =1200) Bankura, West Bengal, India. The affected flock showing severe loss production and sudden death peak summer month June with 4% mortality week. Post the dead birds were performed bacteriological examination done as per standard procedure. At necropsy, had pale...

Staphylococcus aureus is a Gram positive commensal of the mammalian body, commonly found in respiratory tract, skin and mammary gland.Bovine milk major source S. aureus.Various factors play role production pathogenicity bacterium including enzymes like thermonuclease, encoded by nuc gene.The present study was carried out for isolation bovine determination occurrence gene them.Out 168 samples, 146 strains (86.90%) were isolated, which 49 (32.88%) polymerase chain reaction (PCR).In study,...

Escherichia coli is one of the pathogenic bacteria causing food-borne infections like chicken meat, which can lead to serious public health hazards.Antimicrobial resistance in E. a threat human population nowadays.The present study aimed detect extended-spectrum beta-lactamases (ESBLs) and AmpC beta-lactamase (ACBL)producing from different districts West Bengal.A total 107 raw meat samples were collected just after slaughter districts' local markets followed by isolation identification...

Despite Fowl typhoid (FT) eradication in commercial poultry of some parts the world, outbreak threats are not eliminated developing countries. In current study, we reported molecular identification Salmonella gallinarum Indian isolates from backyard by a multiplex PCR assay. Boiled culture lysates Gallinarum (n=2) an showed positive amplifications glgC and speC genes duplex with 174 bp 252 products, respectively. assay, primers for salmonella plasmid virulence gene (spvC) were also added...

Diarrhoea in canines is mainly caused by Escherichia coli which can be fatal also. To understand the depth of this infection, a study was undertaken to detect E. isolates from diarrhoeic and non-diarrhoeic dogs Southern part West Bengal. A total 112 canine samples were tested during May September 2012 revealing approx 63.4% (71) positive for coli. The most common serotypes O8 (23.9%) followed O157 (19.7%), O101 (16.9%), O26 (15.5%), O153 (12.7%) O6 (11.3%). Among these O6, O8, highly...

Sub-clinical Mastitis is one of the major problems in India which among largest milk producing countries world, hampering national economy. Among 422 bovine samples screened from different cattle farms West Bengal with history infection and drop yield by on-spot bromothymol blue (BTB) test, 371 were considered for collection secondary screening somatic cell count study. The collected showed significantly high average SCC value 3.26-4.88 lakh cells/ml was indicative infection. Samples (23,...

Escherichia coli are important commensal with pathogenic potentiality in bovine neonates and may cause intestinal extra-intestinal infections.Different enteric pathogens such as E. remain associated diarrhoea which is a multifactorial menace.Possession of antimicrobial resistance genes by these or diarrhea makes the issue more serious.The study was aimed to detect occurrence characterize extended-spectrum (ESBL) AmpC (ACBL) beta-lactamases producing (STEC) diarrhoeal faecal samples collected...

Background: Escherichia coli and Klebsiella pneumoniae are two important commensals with pathogenic potentiality in animals birds, causing intestinal extra-intestinal infections. These somewhat zoonotic too via different routes. So, the present study was undertaken to investigate prevalence of these bacteria from cloaca environment ducks districts West Bengal, followed by detailed characterization antibiogram. Methods: Around 197 duck environmental samples were collected Bengal. E. K....