A5005592645- Malaria Research and Control
- Mosquito-borne diseases and control
- Toxoplasma gondii Research Studies
- Vector-borne infectious diseases
- Cellular Mechanics and Interactions
- Cardiomyopathy and Myosin Studies
- HIV Research and Treatment
- Invertebrate Immune Response Mechanisms
- Catalytic C–H Functionalization Methods
- Radical Photochemical Reactions
- Photonic and Optical Devices
- Parasites and Host Interactions
- Advanced Fluorescence Microscopy Techniques
- Quantum optics and atomic interactions
- Microbial Metabolism and Applications
- Advanced Fiber Laser Technologies
- Complement system in diseases
- Antibiotic Resistance in Bacteria
- Cold Atom Physics and Bose-Einstein Condensates
- Parasitic Infections and Diagnostics
- Trypanosoma species research and implications
- Parasite Biology and Host Interactions
- Quantum Information and Cryptography
- Identification and Quantification in Food
- Signaling Pathways in Disease
Harvard University
2013-2025
West Bengal University of Animal and Fishery Sciences
2022-2025
Duke University
2023-2024
Indian Institute of Technology Dhanbad
2021-2023
KIIT University
2022
Boston University
2020
Yale University
2007-2009
Osaka University
2005
Efforts to identify host determinants for malaria have been hindered by the absence of a nucleus in erythrocytes, which precludes genetic manipulation cell parasite replicates. We used cultured red blood cells derived from hematopoietic stem carry out forward screen Plasmodium falciparum determinants. found that CD55 is an essential factor P. invasion. CD55-null erythrocytes were refractory invasion all isolates because parasites failed attach properly erythrocyte surface. Thus, attractive...
Calcium Dependent Protein Kinases are key effectors of calcium signaling in malaria parasite. PfCDPK1 is critical for asexual development Plasmodium falciparum, but its precise function and substrates remain largely unknown. Using a conditional knockdown strategy, we here establish that this kinase the invasion host erythrocytes. Furthermore, using multidisciplinary approach involving comparative phosphoproteomics gain insights into underlying molecular mechanisms. We identify PfCDPK1, which...
Scalable classical controllers are a key component of future fault-tolerant quantum computers. Neutral atom computers leverage commercially available optoelectronic devices for generating large-scale tweezer arrays and performing parallel readout, but implementing massively parallel, locally addressed gate operations is an open challenge. In this work, we demonstrate optical modulator system based on off-the-shelf components, which can generate two-dimensional array over 10,000 focused spots...
Measures to combat the parasites that cause malaria have become compromised because of reliance on a small arsenal drugs and emerging drug resistance. We conducted transposon mutagenesis screen in primate parasite Plasmodium knowlesi , producing most complete classification gene essentiality any spp. date, with resolution define truncatable genes. found conservation druggable genome between divergences mitochondrial metabolism. Perturbation analyses frontline antimalarial artemisinin...
Cellular reproduction defines life, yet our textbook-level understanding of cell division is limited to a small number model organisms centered around humans. The horizon on variants expanded here by advancing insights the fascinating modes found in Apicomplexa, key group protozoan parasites. Apicomplexa display remarkable variation offspring number, whether karyokinesis follows each S/M-phase or not, and daughter cells bud cytoplasm from cortex. We find that terminology used describe...
Asexual proliferation of the Plasmodium parasites that cause malaria follows a developmental program alternates non-canonical intraerythrocytic replication with dissemination to new host cells. We carried out functional analysis falciparum homolog Protein Phosphatase 1 (PfPP1), universally conserved cell cycle factor in eukaryotes, investigate regulation parasite proliferation. PfPP1 is indeed required for efficient replication, but absolutely essential egress from red blood By...
The study was conducted to detect the occurrence and phenotypic resistance pattern of ESBL-producing Enterobacteriaceae in livestock using docking based analysis reveal classes antibiotics against which ESBL-producers are active. Rectal swabs from healthy cattle (n=100), goats (n=88), pigs (n=66) were collected backyard farms Andaman Nicober island (India). In total, 304 isolates comprising E. coli (131), Salmonella (75), Klebsiella penumoniae (98) recovered. showed that highest found...
<title>Abstract</title> Digital holographic microscopy (DHM) has emerged as a powerful, label-free technique for visualizing and analyzing biological samples. By extracting the intrinsic optical properties of red blood cells (RBCs), DHM enables detection infection-induced morphological biophysical changes. Traditional classification methods often rely on feature-specific analysis, which can lead to misclassification when single parameter fails differentiate between uninfected infected cells....
Formin-homology (FH) 2 domains from formin proteins associate processively with the barbed ends of actin filaments through many rounds subunit addition before dissociating completely. Interaction monomer-binding protein profilin FH1 domain speeds processive end elongation by FH2 domains. In this study, we examined energetic requirements for fast elongation. contrast to previous proposals, direct microscopic observations single molecules Bni1p Saccharomyces cerevisiae labeled quantum dots...
ABSTRACT The apicomplexan parasites that cause malaria and babesiosis invade proliferate within erythrocytes. To assess the potential for common antiparasitic treatments, we measured sensitivities of multiple species Plasmodium Babesia to chemically diverse collection antimalarial compounds in Malaria Box library. We observed these share a large fraction same inhibitors identified with strong babesiacidal activity.
Exploitation of the visible light-induced photoreactivity an electron donor–acceptor complex indoles and a hypervalent iodine compound led to development methodologies for synthesis 3-acetoxyindoles isatins.
Abstract Apicomplexan egress from host cells is fundamental to the spread of infection and poorly characterized in Babesia spp., parasites veterinary importance emerging zoonoses. Through use video microscopy, transcriptomics chemical genetics, we have implicated signaling, proteases gliding motility as key drivers by divergens . We developed reverse genetics perform a knockdown screen putative mediators egress, identifying kinases involved distinct steps (ASP3, PKG CDPK4) invasion (ASP2,...
PML5 is a functional derivative of family I.3 lipase from Pseudomonas sp. MIS38 and contains five repeats nine‐residue sequence motif. Two aspartate residues within the second third repetitive sequences were replaced by Ala. The secretion level, intracellular accumulation stability resultant mutant protein greatly reduced as compared to those PML5. In addition, this was inactive did not bind Ca 2+ ion. We propose that form β‐roll structure in cells thereby contribute efficiency protein.
Red blood cell invasion by the malaria parasite Plasmodium falciparum relies on a complex protein network that uses low and high affinity receptor–ligand interactions. Signal transduction through action of specific kinases is control mechanism for orchestration this process. In present study we report phosphorylation CPD (cytoplasmic domain) P. Rh2b (reticulocyte homologue 2b). First, identified Ser3233 as sole phospho-acceptor site in vitro extract. We provide several lines evidence...
Babesiosis is an emerging zoonosis and widely distributed veterinary infection caused by 100+ species of Babesia parasites. The diversity parasites the lack specific drugs necessitate discovery broadly effective antibabesials. Here, we describe a comparative chemogenomics (CCG) pipeline for identification conserved targets. CCG relies on parallel in vitro evolution resistance independent populations spp. ( B. bovis divergens ). We identified potent antibabesial, MMV019266, from Malaria Box,...