A5029235430- Toxoplasma gondii Research Studies
- Glaucoma and retinal disorders
- Ubiquitin and proteasome pathways
- Ocular Oncology and Treatments
- Autophagy in Disease and Therapy
- Ocular Diseases and Behçet’s Syndrome
- Ocular Infections and Treatments
- Immunotherapy and Immune Responses
- Retinal and Optic Conditions
- Cancer Immunotherapy and Biomarkers
- CRISPR and Genetic Engineering
- Cytomegalovirus and herpesvirus research
- Mosquito-borne diseases and control
- Malaria Research and Control
- Single-cell and spatial transcriptomics
- Neurological Disorders and Treatments
- RNA Interference and Gene Delivery
- Immune cells in cancer
- Cancer-related Molecular Pathways
- Cell death mechanisms and regulation
- Trypanosoma species research and implications
- Cell Adhesion Molecules Research
- Retinoids in leukemia and cellular processes
- Retinal Diseases and Treatments
Whitehead Institute for Biomedical Research
2016-2023
Massachusetts Institute of Technology
2014
Massachusetts Eye and Ear Infirmary
2008-2011
Harvard University
2008-2011
Smith-Kettlewell Eye Research Institute
2009-2011
Toxoplasma gondii is a parasite of humans and animals, model for other apicomplexans including Plasmodium spp., the causative agents malaria. Despite many advances, manipulating T. genome remains labor intensive, often restricted to lab-adapted strains or lines carrying mutations that enable selection. Here, we use RNA-guided Cas9 nuclease efficiently generate knockouts without selection, introduce point epitope tags into genome. These methods will streamline functional analysis genes...
Glaucoma, the most frequent optic neuropathy, is a leading cause of blindness worldwide. Death retinal ganglion cells (RGCs) occurs in all forms glaucoma and accounts for loss vision, however molecular mechanisms that RGC remain unclear. The pro-apoptotic molecule, Fas ligand, transmembrane protein can be cleaved from cell surface by metalloproteinases to release soluble with antagonistic activity. Previous studies documented constitutive ocular expression FasL maintained immune privilege...
This work uncovers interactions between various signaling pathways that govern Toxoplasma gondii egress. Specifically, we compare the function of three canonical calcium-dependent protein kinases (CDPKs) using chemical-genetic and conditional-depletion approaches. We describe a previously uncharacterized CDPK, CDPK2A, in lytic cycle, demonstrating it contributes to parasite fitness through regulation microneme discharge, gliding motility, egress from infected host cells. Comparison...
ABSTRACT The transition between parasite replication and dissemination is regulated in apicomplexan parasites by fluctuations cytosolic calcium concentrations, effectuated calcium-dependent protein kinases (CDPKs). We examined the role of CDPK2A lytic cycle Toxoplasma , analyzing its regulation cellular processes associated with motility. used chemical-genetic approaches conditional depletion to determine that contributes initiation motility through microneme discharge. demonstrate...