Divyashree Kushnoor
A5089050112- Endometriosis Research and Treatment
- Reproductive System and Pregnancy
- Cancer Immunotherapy and Biomarkers
- Single-cell and spatial transcriptomics
- Endometrial and Cervical Cancer Treatments
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Immune responses and vaccinations
- Immune cells in cancer
- Immune Cell Function and Interaction
- Lung Cancer Research Studies
- RNA Research and Splicing
- COVID-19 Impact on Reproduction
- Long-Term Effects of COVID-19
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Cancer Genomics and Diagnostics
- Molecular Biology Techniques and Applications
- Legume Nitrogen Fixing Symbiosis
- T-cell and B-cell Immunology
University of California, San Francisco
2020-2024
Although infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has pleiotropic and systemic effects in some individuals1-3, many others experience milder symptoms. Here, to gain a more comprehensive understanding of the distinction between mild phenotypes pathology disease 2019 (COVID-19) its origins, we performed whole-blood-preserving single-cell analysis protocol integrate contributions from all major immune cell types blood-including neutrophils, monocytes,...
Endometriosis is a chronic, estrogen-dependent disorder where inflammation contributes to disease-associated symptoms of pelvic pain and infertility. Immune dysfunction includes insufficient immune lesion clearance, pro-inflammatory endometrial environment, systemic inflammation. Comprehensive understanding endometriosis pathophysiology in different hormonal milieu disease severity has been hampered by limited direct characterization populations endometrium, blood, lesions. Simultaneous deep...
Background Colitis caused by checkpoint inhibitors (CPI) is frequent and treated with empiric steroids, but CPI colitis mechanisms in steroid-experienced or refractory disease are unclear. Methods Using colon biopsies blood from predominantly patients, we performed multiplexed single-cell transcriptomics proteomics to nominate contributing populations. Results showed enrichment of CD4 + resident memory (RM) T cells addition CD8 RM cytotoxic cells. Matching cell receptor (TCR) clonotypes...
Abstract While SARS-CoV-2 infection has pleiotropic and systemic effects in some patients, many others experience milder symptoms. We sought a holistic understanding of the severe/mild distinction COVID-19 pathology, its origins. performed wholeblood preserving single-cell analysis protocol to integrate contributions from all major cell types including neutrophils, monocytes, platelets, lymphocytes contents serum. Patients with mild disease display coordinated pattern interferonstimulated...
SUMMARY Cancers display significant heterogeneity with respect to tissue of origin, driver mutations and other features the surrounding tissue. It is likely that persistent tumors differentially engage inherent patterns–here ‘Archetypes’–of immune system, both benefit from a tumor microenvironment (TIME) disengage tumor-targeting. To discover dominant system archetypes, Immunoprofiler Initiative (IPI) processed 364 individual across 12 cancer types using standardized protocols. Computational...
Cancers display significant heterogeneity with respect to tissue of origin, driver mutations and other features the surrounding tissue. It is likely that persistent tumors differentially engage inherent patterns–here ‘Archetypes’–of immune system, both benefit from a tumor microenvironment (TIME) disengage tumor-targeting. To discover dominant system archetypes, Immunoprofiler Initiative (IPI) processed 364 individual across 12 cancer types using standardized protocols. Computational...
Abstract While SARS-CoV-2 infection has pleiotropic and systemic effects in some patients, many others experience milder symptoms. We sought a holistic understanding of the severe/mild distinction COVID-19 pathology, its origins. performed whole-blood preserving single-cell analysis protocol to integrate contributions from all major cell types including neutrophils, monocytes, platelets, lymphocytes contents serum. Patients with mild disease display coordinated pattern interferon-stimulated...
Abstract Background Endometriosis is a chronic, estrogen-dependent disorder where inflammation contributes to disease-associated symptoms of pelvic pain and infertility. Immune dysfunction includes insufficient immune lesion clearance, pro-inflammatory endometrial environment, systemic inflammation. Comprehensive understanding endometriosis pathophysiology in different hormonal milieu disease severity has been hampered by limited direct characterization populations endometrium, blood,...
Purpose To detail the workflow for Multiome coassay of 20 PBMC samples with 3 technicians using 10X Next GEM Single Cell ATAC + Gene Expression Kit Please refer to attached template as mentioned in protocol