A5007014906- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Berry genetics and cultivation research
- Plant Pathogens and Resistance
- Genetic diversity and population structure
- Malaria Research and Control
- Helminth infection and control
- Antimicrobial Peptides and Activities
- Plant Reproductive Biology
- Amphibian and Reptile Biology
- Genomics and Phylogenetic Studies
- Plant Disease Management Techniques
- Evolution and Genetic Dynamics
- Vector-borne infectious diseases
- Plant Virus Research Studies
- Invertebrate Immune Response Mechanisms
- Mosquito-borne diseases and control
- Genetic and phenotypic traits in livestock
- Chromosomal and Genetic Variations
- Plant Taxonomy and Phylogenetics
- Plant tissue culture and regeneration
- Genetic Associations and Epidemiology
- Trace Elements in Health
- Aquaculture disease management and microbiota
- Biochemical and Structural Characterization
Broad Institute
2020-2025
Harvard University
2019-2025
Harvard University Press
2025
Oregon State University
2013-2023
University of Washington
2010-2012
Cornell University
2003
As a first step toward understanding how rare variants contribute to risk for complex diseases, we sequenced 15,585 human protein-coding genes an average median depth of 111× in 2440 individuals European (n = 1351) and African 1088) ancestry. We identified over 500,000 single-nucleotide (SNVs), the majority which were (86% with minor allele frequency less than 0.5%), previously unknown (82%), population-specific (82%). On average, 2.3% 13,595 SNVs each person carried predicted affect protein...
Whole-genome duplications are radical evolutionary events that have driven speciation and adaptation in many taxa.Higher-order polyploids complex histories often including interspecific hybridization dynamic genomic changes.This chromosomal reshuffling is poorly understood for most polyploid species, despite their agricultural importance, due to the challenge of distinguishing homologous sequences from each other.Here, we use dense linkage maps generated with targeted sequence capture...
Measures to combat the parasites that cause malaria have become compromised because of reliance on a small arsenal drugs and emerging drug resistance. We conducted transposon mutagenesis screen in primate parasite Plasmodium knowlesi , producing most complete classification gene essentiality any spp. date, with resolution define truncatable genes. found conservation druggable genome between divergences mitochondrial metabolism. Perturbation analyses frontline antimalarial artemisinin...
Turnovers of sex-determining systems represent important diversifying forces across eukaryotes. Shifts in sex chromosomes—but conservation the master genes—characterize distantly related animal lineages. Yet plants, which separate sexes have evolved repeatedly and chromosomes are typically homomorphic, we do not know whether such translocations drive sex-chromosome turnovers within closely taxonomic groups. This phenomenon can only be demonstrated by identifying sex-associated nucleotide...
Control and elimination of schistosomiasis is an arduous task, with current strategies proving inadequate to break transmission. Exploration genetic approaches interrupt Schistosoma mansoni transmission, the causative agent for human intestinal in sub-Saharan Africa South America, has led genomic research snail vector hosts genus Biomphalaria. Few complete resources exist, African Biomphalaria species being particularly underrepresented despite this where majority S. infections occur. Here...
Introduced species often colonize regions that have vastly different ecological and environmental conditions than those found in their native range. As such, introductions can provide a deeper understanding into the process of adaptive evolution. In 1880s, steelhead trout (Oncorhynchus mykiss) from California were introduced Lake Michigan (Laurentian Great Lakes, North America) where they established naturally reproducing populations. range, hatch rivers, migrate to ocean return freshwater...
Schistosomiasis, a neglected global pandemic, may be curtailed by blocking transmission of the parasite via its intermediate hosts, aquatic snails. Elucidating genetic basis snail-schistosome interaction is key to this strategy. Here we map natural parasite-resistance polymorphism from Caribbean population snail Biomphalaria glabrata. In independent experimental evolution lines, RAD genotyping shows that same genomic region responds selection for resistance Schistosoma mansoni. A dominant...
• Premise of the study: Molecular phylogenies derived from all three plant genomes can provide insight into evolutionary history groups influenced by reticulation. We sought to reconstruct mitochondrial exome, chloroplast, and nuclear genome for octoploid Fragaria their diploid ancestors document patterns incongruence between within cytoplasmic interpret these in context origin strawberries. Methods: Using a genome‐skimming approach, we assembled chloroplast exomes, used POLiMAPS method...
Abstract Gynodioecy is a sexual system wherein females coexist with hermaphrodites. It of interest not only because male-sterile plants are advantageous in plant breeding but also it can be crucial step the evolutionary transition to entirely separate sexes (dioecy) from hermaphroditic ancestor. The gynodioecious diploid wild strawberry, Fragaria vesca ssp. bracteata (Rosaceae), member clade both dioecious and cultivated species, making an ideal model which study genetics male sterility. To...
Schistosomiasis is one of the most important neglected tropical diseases. Despite effective chemotherapeutic treatments, this disease continues to afflict hundreds millions people. Understanding natural intermediate snail hosts schistosome parasites vital suppression disease. A recently identified genomic region in Caribbean Biomphalaria glabrata snails strongly influences their resistance infection by Schistosoma mansoni. This contains novel genes having structural similarity known pathogen...
<title>Abstract</title> Schistosomiasis, a neglected tropical disease, is transmitted by freshwater snails. Interruption of transmission will require novel vector-focused interventions. We performed genome-wide association study African snails, <italic>Biomphalaria sudanica</italic>, exposed to <italic>Schistosoma mansoni</italic> in an endemic area high Kenya. Two snail genomic regions, SudRes1 and SudRes2, were significantly associated with immunity schistosomes. includes receptor-like...
Biomphalaria snails are intermediate hosts for schistosome parasites, which cause morbidity and mortality in humans worldwide. We aimed to determine the mating system of sudanica, a hermaphroditic vector schistosomiasis African Great Lakes, with goal informing design genetic studies such as linkage mapping improve genome assembly association identify snail resistance genes. To relative rates outcrossing versus selfing, we assayed progeny experimental crosses laboratory using PCR restriction...
ABSTRACT Vector control remains an important strategy worldwide to prevent human infection with pathogens transmitted by arthropods. strategies rely on accurate identification of vector taxa along vector‐specific biological indicators such as feeding ecology, prevalence and insecticide resistance. Multiple ‘DNA barcoding’ protocols have been published over the past several decades support these applications, generally relying informal manual approaches BLAST assign taxonomic identity...
ABSTRACT Malaria in South America remains a serious public health problem. Anopheles ( Nyssorhynchus ) darlingi is the most important malaria vector across tropical Latin America. Vector-targeted disease control efforts require thorough understanding of mosquito demographic and evolutionary patterns. We present analyze whole genomes 1094 A. (median depth 18x) from six American countries. observe deep geographic population structure, high genetic diversity including thirteen putative...
Few genetic differences between human populations conform to the classic model of positive selection, in which a newly arisen mutation rapidly approaches fixation one lineage, suggesting that adaptation more commonly occurs via moderate changes standing variation at many loci. Detecting and characterizing this type complex selection requires integrating individually ambiguous signatures across genomically geographically extensive data. Here, we develop novel approach test hypothesis has...
Abstract Motivation: The goal of any parentage analysis is to identify as many parent–offspring relationships possible, while minimizing incorrect assignments. Existing methods can achieve these ends, but they require additional information in the form demographic data, thousands markers and/or estimates genotyping error rates. For non-model systems, it simply not practical, cost-effective or logistically feasible obtain this information. Here, we develop a Bayesian method that only requires...
Background New strategies to combat the global scourge of schistosomiasis may be revealed by increased understanding mechanisms which obligate snail host can resist schistosome parasite. However, few molecular markers linked resistance have been identified and characterized in snails. Methodology/Principal Findings Here we test six independent genetic loci for their influence on Schistosoma mansoni strain PR1 13-16-R1 Biomphalaria glabrata. We first identify a genomic region, RADres, showing...
Recombination in ancient, heteromorphic sex chromosomes is typically suppressed at the sex-determining region (SDR) and proportionally elevated pseudoautosomal (PAR). However, little known about recombination dynamics of young, homomorphic plant chromosomes. We examine male female function crosses unrelated samples dioecious octoploid strawberry Fragaria chiloensis order to map small recently evolved SDR controlling both traits patterns on incipient ZW chromosome. The this system located...
Premise of the Study Recurrent formation polyploid taxa is a common observation in many plant groups. Haploid, cytoplasmic genomes like plastid genome can be used to overcome problem homeologous genes and recombination taxa. Fragaria (Rosaceae) contains several octo‐ decaploid species. We use plastome sequences infer ancestry these with special focus on cascadensis . Methods skimming 96 samples single Illumina HiSeq 3000 lane obtain whole sequences. These were for phylogenetic...
Insecticide resistance is a major threat to gains in malaria control, which have been stalling and potentially reversing since 2015. Studies into the causal mechanisms of insecticide are painting an increasingly complicated picture, underlining need design implement targeted studies on this phenotype. In study, we compare three populations vector An. coluzzii: susceptible two resistant colonies with same genetic background. The original colonised population rapidly lost over 6-month period,...
Balancing selection is common on many defense genes, but it has rarely been reported for immune effector proteins such as antimicrobial peptides (AMPs). We describe genetic diversity at a brevinin-1 AMP locus in three species of leopard frogs (Rana pipiens, Rana blairi, and palustris). Several highly divergent allelic lineages are segregating this locus. That unusual pattern results from balancing demonstrated by multiple lines evidence, including ratio nonsynonymous/synonymous polymorphism...