A5070605015- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Cancer, Hypoxia, and Metabolism
- Pluripotent Stem Cells Research
- Cancer Genomics and Diagnostics
- Cancer Research and Treatments
- Epigenetics and DNA Methylation
- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Fibroblast Growth Factor Research
- Cytokine Signaling Pathways and Interactions
- Molecular Biology Techniques and Applications
- Hippo pathway signaling and YAP/TAZ
- Pancreatic and Hepatic Oncology Research
- Wnt/β-catenin signaling in development and cancer
- Axon Guidance and Neuronal Signaling
- Nanofabrication and Lithography Techniques
- Tissue Engineering and Regenerative Medicine
- Ion-surface interactions and analysis
- Cancer-related gene regulation
- Advanced Proteomics Techniques and Applications
- Bone Tissue Engineering Materials
Princess Margaret Cancer Centre
2020-2024
University of Toronto
2015-2024
University Health Network
2020-2022
Toronto Public Health
2015-2017
State Street (United States)
2017
Micropatterning strategies, which enable control over cell and tissue architecture in vitro, have emerged as powerful platforms for modelling microenvironments at different scales complexities. Here, we provide an overview of popular micropatterning techniques, along with detailed descriptions, to guide new users through the decision making process procedure use, how best obtain desired patterns. Example techniques types biological observations that can be made are provided from literature....
Targeted therapies require life-long treatment, as drug discontinuation invariably leads to tumor recurrence. Recurrence is mainly driven by minor subpopulations of drug-tolerant persister (DTP) cells that survive the cytotoxic effect. In lung cancer, DTP studies have been conducted with cell line models.We an in vivo study using a adenocarcinoma patient-derived xenograft EGFR mutation. Daily treatment tumor-bearing mice for 5 6 weeks inhibitor erlotinib markedly shrunk tumors and generated...
Squamous cell carcinomas constitute a major class of head & neck cancers, where the tumour stroma ratio (TSR) carries prognostic information. Patients affected by stroma-rich tumours exhibit poor prognosis and higher chance relapse. As such, there is need for technology platform that allows rapid determination ratio. In this work, we provide proof-of-principle demonstration Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) can be used to determine ratios. Slices from three...
Separation of phenotypically distinct cell populations is necessary to ensure proper organization and function tissues organs therefore understanding fundamental mechanisms that drive this segregation important. In work, authors present an<italic>in vivo</italic>model system accurately recapitulates important aspects segregation<italic>in vivo</italic>and allows dissection behaviours driving segregation.
Abstract Background: Cancer-associated fibroblasts (CAFs) are heterogeneous, with CAF subtypes being localized in spatially defined areas of tumors. However, it remains unclear which subsets specifically play a role fundamental processes such as tumor growth, invasion, and response to therapy. This study aims establish novel vitro model that recapitulates the vivo tumor-stroma spatial relationship investigate heterogeneity its impact on cell growth non-small lung cancer (NSCLC). Methods: The...
Compartment boundaries are essential for ensuring proper cell organization during embryo development and in adult tissues, yet the mechanisms underlying boundary establishment not completely understood.
Abstract Cancer-associated fibroblasts (CAFs) have previously been shown to play a pivotal role in multiple cancer dynamics, including mediating tumor cell invasion: their pro-invasive secretory profile and ability remodel the extracellular matrix (ECM) architecture particularly promote progression through invasion into surrounding tissue areas beyond. Given that reduced CAF abundance tumors correlates with improved outcomes various cancers, we set out identify epigenetic targets involved...
Chemically directed differentiation of pluripotent stem cells (PSCs) into defined cell types is a potent strategy for creating regenerative tissue models and therapies. In vitro observations suggest that physical cues can augment differentiation. We recently demonstrated confining human PSC-derived lung progenitor in tube with diameter mimics those observed during development results the alteration towards SOX2-SOX9+ cells. Here we set out to assess robustness this geometric confinement...
Abstract The interface between a tumour and the adjacent stroma is site of great importance for development. At this site, carcinoma cells are highly proliferative, undergo invasive phenotypic changes, directly interact with surrounding stromal cells, such as cancer-associated fibroblasts (CAFs) which further exert pro-tumorigenic effects. Here we describe development GLAnCE (Gels Live Analysis Compartmentalized Environments), an easy-to-use hydrogel-culture platform investigating CAF-tumour...