A5081147577- Retinal Development and Disorders
- Visual perception and processing mechanisms
- Color Science and Applications
- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Neural dynamics and brain function
- Optical Imaging and Spectroscopy Techniques
- CCD and CMOS Imaging Sensors
- Orbital Angular Momentum in Optics
- melanin and skin pigmentation
- Photonic and Optical Devices
- Ocular and Laser Science Research
- Optical Polarization and Ellipsometry
- Antioxidant Activity and Oxidative Stress
- Circadian rhythm and melatonin
- Optical Coatings and Gratings
- Mechanical and Optical Resonators
University of Rochester
2018-2024
Instituto de Óptica "Daza de Valdés"
2024
Center for Visual Communication (United States)
2024
University of Rochester Medical Center
2021
Rochester Institute of Technology
2013
Optogenetic therapies for vision restoration aim to confer intrinsic light sensitivity retinal ganglion cells when photoreceptors have degenerated and has been irreversibly lost. We combine adaptive optics ophthalmoscopy with calcium imaging optically record optogenetically restored cell activity in the fovea of living primate. Recording from intact eye a animal, we compare patterns evoked by optogenetic actuator ChrimsonR natural photoreceptor mediated stimulation same cells. responses are...
The primate foveola, with its high cone density and magnified cortical representation, is exquisitely specialized for high-resolution spatial vision. However, uncovering the wiring of retinal circuitry responsible this performance has been challenging due to difficulty in recording receptive fields foveal ganglion cells (RGCs) vivo. In study, we use adaptive optics scanning laser ophthalmoscopy (AOSLO) image calcium responses RGCs living primate, a stable, precision visual stimulus that...
The primate fovea is specialized for high acuity chromatic vision, with the highest density of cone photoreceptors and a disproportionately large representation in visual cortex. unique properties conferred by are conveyed to brain retinal ganglion cells, somas which lie at margin foveal pit. Microelectrode recordings these centermost cells have been challenging due fragility excised retina. Here we overcome this challenge combining resolution fluorescence adaptive optics ophthalmoscopy...
A long-standing question in vision science is how the three cone photoreceptor types – long (L), medium (M) and short (S) wavelength sensitive combine to generate our perception of color. Hue can be described along two opponent axes: red-green blue-yellow. Psychophysical measurements color appearance indicate that inputs blue-yellow axes are M vs. L+S L M+S, respectively. However, “cardinal directions space” revealed by psychophysical detection thresholds following adaptation S L+M. These...
All retina-based vision restoration approaches rely on the assumption that photoreceptor loss does not preclude reactivation of remaining retinal architecture. Whether extended periods limit efficacy restorative therapies at level is unknown. We examined long-term changes in optogenetic responsivity foveal ganglion cells (RGCs) non-human primates following localized ablation by high-intensity laser exposure. By performing fluorescence adaptive optics scanning light ophthalmoscopy (AOSLO)...
We present a ray transfer matrix for spiral phase plate. Using this we determine the stability of an optical resonator made two plates and trace stable orbits in resonator. Our results should be relevant to laser physics, micromanipulation, quantum information, optomechanics.
A long-standing question in vision science is how the three cone photoreceptor types – long (L), medium (M) and short (S) wavelength sensitive combine to generate our perception of color. Hue can be described along two opponent axes: red-green blue-yellow. Psychophysical measurements color appearance indicate that inputs blue-yellow axes are M vs. L+S L M+S, respectively. However, “cardinal directions space” revealed by psychophysical detection thresholds S L+M. The cardinal match most...
While the fovea is best known for its high spatial acuity mediated by a density of midget ganglion cells (RGCs), anatomy and transcriptomics show ~15 rarer RGC types are also present. However, our understanding visual information these RGCs convey to brain limited as both have been difficult address with standard physiology techniques. We addressed this gap in knowledge vivo, two macaques expressing GCaMP6s foveal cell layer. Using fluorescence adaptive optics scanning light ophthalmoscope,...
Here, we optically record responses to spatial and chromatic stimuli using a calcium indicator in the living macaque eye characterize receptive field (RF) properties of retinal ganglion cells (RGCs) serving foveal center. GCaMP6s was expressed three female macaques. Adaptive optics ophthalmoscopy used image fluorescence (488nm ex, 520/35nm em) from RGCs whose RF centers were driven by cones central 36 arcmin fovea additional 6 foveola. Using cone isolating luminance flicker (1.3deg, 0.15Hz,...
Get PDF Email Share with Facebook Tweet This Post on reddit LinkedIn Add to CiteULike Mendeley BibSonomy Citation Copy Text M. Eggleston, T. Godat, E. Munro, A. Alonso, H. Shi, and Bhattacharya, "Ray transfer matrix for a spiral phase plate," in Frontiers Optics 2013, I. Kang, D. Reitze, N. Alic, Hagan, eds., OSA Technical Digest (online) (Optical Society of America, 2013), paper FTu5F.1. Export BibTex Endnote (RIS) HTML Plain alert Save article
Despite the significance of fovea for primate vision, its physiology is much less understood than peripheral retina. We functionally classified foveal cells in ganglion cell layer (GCL) a female macaque that express genetically encoded calcium indicator (GCaMP6s), allowing optical recording responses to chromatic and luminance flicker. Adaptive optics scanning light ophthalmoscopy (AOSLO) was used image GCaMP6 fluorescence (488 nm excitation, 500–540 emission) from ring displaced GCL driven...
Abstract The primate fovea is specialized for high acuity chromatic vision, with the highest density of cone photoreceptors and a disproportionately large representation in visual cortex. unique properties conferred by are conveyed to brain retinal ganglion cells, somas which lie at margin foveal pit. Microelectrode recordings these centermost cells have been challenging due fragility excised retina. Here we overcome this challenge combining resolution fluorescence adaptive optics...