A5087739105- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Circadian rhythm and melatonin
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Advanced Breast Cancer Therapies
- Advanced Fluorescence Microscopy Techniques
- Ferroptosis and cancer prognosis
- Genetic factors in colorectal cancer
- MicroRNA in disease regulation
- Cancer Genomics and Diagnostics
- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- Neuroscience and Neural Engineering
- Circular RNAs in diseases
- Osteoarthritis Treatment and Mechanisms
- Bone and Dental Protein Studies
- Esophageal Cancer Research and Treatment
- Immune cells in cancer
- Polymer-Based Agricultural Enhancements
- BRCA gene mutations in cancer
- Hematopoietic Stem Cell Transplantation
- Phytochemistry and Bioactivity Studies
- Sinusitis and nasal conditions
University of Rochester
2021-2024
Ningbo University
2021-2024
Henan Agricultural University
2024
Zhejiang University
2024
Second Affiliated Hospital of Zhejiang University
2024
Instituto de Óptica "Daza de Valdés"
2023
Center for Visual Communication (United States)
2023
Hainan Medical University
2021-2023
Academy of Military Medical Sciences
2021-2022
Bengbu Medical College
2019
A comprehensive understanding of the cellular heterogeneity and molecular mechanisms underlying development, homeostasis, disease human intervertebral disks (IVDs) remains challenging. Here, transcriptomic landscape 108 IVD cells was mapped using single-cell RNA sequencing three main compartments from young adult healthy IVDs, including nucleus pulposus (NP), annulus fibrosus, cartilage endplate (CEP). The chondrocyte subclusters were classified based on their potential regulatory,...
Cisplatin resistance is a major obstacle in the treatment of lung adenocarcinoma (LAD), and its mechanism has not been fully elucidated. Here, we report that miR-326 downregulated cisplatin-resistant A549/CDDP cells compared with parental A549 cells. Overexpression reversed cisplatin chemoresistance LAD vitro vivo. Moreover, identified specificity protein 1 (SP1) gene as novel direct target miR-326. Knockdown SP1 revealed similar effects ectopic expression. Decreased expression was also...
Purpose: Mutations leading to homologous recombination deficiency (HRD) increase the tumor sensitivity platinum-based chemotherapy and PARP inhibitors. However, reversion mutations often develop conferring acquired drug resistance. There is still a lack of comprehensive investigation on HRR in large pan-cancer cohorts, especially Eastern Asian population. This study aims characterize repair (HRR)-related genes cohort Chinese patients. Methods: Sequencing data from 23,375 patients across over...
Granulocyte colony-stimulating factor (G-CSF) has been widely used to mobilize bone marrow hematopoietic stem/progenitor cells for transplantation in the treatment of hematological malignancies decades. Additionally, G-CSF is also accepted as an essential mediator immune regulation, leading reduced graft-versus-host disease following transplantation. Despite important clinical roles G-CSF, a comprehensive, unbiased, and high-resolution survey into cellular molecular ecosystem human...
All retina-based vision restoration approaches rely on the assumption that photoreceptor loss does not preclude reactivation of remaining retinal architecture. Whether extended periods limit efficacy restorative therapies at level is unknown. We examined long-term changes in optogenetic responsivity foveal ganglion cells (RGCs) non-human primates following localized ablation by high-intensity laser exposure. By performing fluorescence adaptive optics scanning light ophthalmoscopy (AOSLO)...
Background: The clinical outcomes of chemotherapy (CT) for the treatment metastatic triple-negative (TN) and hormone receptor-positive (HR+)/human epidermal growth factor receptor 2-negative (HER2-) breast cancer (mBC) have proven to be disappointing. phosphatidylinositol 3-kinase/protein kinase B/mammalian target rapamycin (PI3K/AKT/mTOR) pathway, a tumor-promoting signaling cascade frequently mutated in (BC), has been implicated chemoresistance. In this study, our objective is investigate...
Objective or purposePhysiological changes in retinal ganglion cells (RGCs) have been reported rodent models of photoreceptor (PR) loss but this has not investigated primates. By expressing both a calcium indicator (GCaMP6s) and an optogenetic actuator (ChrimsonR) foveal RGCs the macaque, we reactivated vivo assessed their response weeks years following PR loss.DesignWe used imaging approach to record optogenetically evoked activity deafferented primate fovea. Cellular scale recordings were...
Chronic rhinosinusitis (CRS) is a complex condition brought on for many reasons, and its prevalence rising gradually around the world. Xanthii Fructus (XF) has been used in treatment of CRS decades effective. The chemical pharmacological profiles XF, other hand, are still unknown need to be clarified. potential mechanisms XF were investigated using network pharmacology approach this study. OB DL charge screening bioactive components drug-likeness. TCMSP PubChem databases identify prospective...
The aim of this study was to explore the regulatory role epoxide hydrolase 3 (EPHX3) in head and neck squamous cell carcinoma (HNSCC) analyze its bioinformatic function, as well as, screen predict miRNAs that can regulate EPHX3 expression HNSCC. We examined profile prognostic potential TCGA GTEX databases performed functional enrichment analysis using string database. Subsequently, we analyzed on EPHX3, including analysis, correlation survival analysis. In addition, also used TIMER...
Abstract Purpose MYL2, a potential biomarker, is aberrantly expressed in variety of tumor tissues. The purpose this study was to determine the exact involvement MYL2 HNSC. Methods Massive bioinformatics analysiswere used assess mRNA and protein expression levels its functional role Next, prognostic effect on HNSC analyzed using TCGA GEPIA. Afterwards, immune microenvironment examined by infiltration monomolecular analysis. Finally, immunohistochemistry, western blot, RT-PCR, Plasmid...
This study investigated the role of miRNA-144 (miR-144) targeting long noncoding DNAJC3-AS1 in regulating breast cancer chemosensitivity. Real-time quantitative polymerase chain reaction was employed to detect levels miR-144 different drug-resistant cells. MTT assays were used measure proliferation cells treatment groups. The apoptosis rate transfected detected by flow cytometry. Western blotting protein and autophagy-related proteins. A double luciferase report experiment performed evaluate...
To explore the effect of bone morphogenetic protein 4(BMP4) on cell cycle and apoptosis hemaropoictic stem progenitor cells (HSPC) in conditions 5-fluorouracil (5-FU)-inducing marrow suppression stress hemogenesis, its possible mechanism.The C57BL transgenic mice with BMP4 overexpression were established enrolled group (BMP4 group), at same time wild type matching age, sex body weight selected control (WT group). The was induced by injection 5-FU dose 150 mg/kg, then nucleated isolated from...
Abstract The significance of MYL2 as a possible HNSC treatment gene is unknown, the purpose this study was to determine exact involvement in HNSC.The Tumor Immune Estimate Resource (TIMER), UALCAN, Cancer Genome Atlas (TCGA), and Gene Expression Omnibus (GEO) databases were used assess mRNA protein expression levels HNSC. Experimental tests, including immunohistochemistry, corroborate findings using tumor tissues normal controls. Next, prognostic effect on analyzed TCGA GEPIA. Understanding...
Cystatin E/M (CST6), a representative cysteine protease inhibitor, plays both tumor-promoting and tumor-suppressing functions is pursued as an epigenetically therapeutic target in special cancer types. However, comprehensive systematic analysis for CST6 pan-cancer level still lacking. In the present study, we explored expression pattern of multiple types across ∼10,000 samples from TCGA (The Cancer Genome Atlas) ∼8,000 MMDs (Merged Microarray-acquired Datasets). We found that dynamic...
Abstract Objective or purpose Physiological changes in retinal ganglion cells (RGCs) have been reported rodent models of photoreceptor (PR) loss but this has not investigated primates. By expressing both a calcium indicator (GCaMP6s) and an optogenetic actuator (ChrimsonR) foveal RGCs the macaque, we reactivated vivo assessed their response weeks years following PR loss. Design We used imaging approach to record optogenetically evoked activity deafferented primate fovea. Cellular scale...
Though the responses of rich variety retinal ganglion cells (RGCs) reflect totality visual processing in retina and provide sole conduit for those processed to brain, we have much learn about how brain uses these signals guide behavior. An impediment developing a comprehensive understanding role circuits behavior is paucity causal studies intact primate system. Here demonstrate ability optogenetically activate individual RGCs with flashes light focused on single RGC somas vivo , without...
The perceptual roles of most retinal ganglion cell (RGC) classes are controversial in part because we lack a paradigm for measuring the consequences activating cells only one class living primate eye. Recent success classifying single RGCs with vivo calcium imaging and optogenetic activation en masse now bring such within reach. As first step, demonstrate stimulation foveal macaque. an anesthetized female macaque were stimulated imaged using adaptive optics scanning light ophthalmoscopy....
Vision restoration therapies aim to restore light sensitivity the retina following photoreceptor (PR) degeneration. Physiological changes in retinal ganglion cells (RGCs) have been reported rodent models of PR loss but this has not investigated primates. By expressing both a calcium indicator (GCaMP6s) and an optogenetic actuator (ChrimsonR) foveal RGCs macaque, we reactivate assess activity weeks years loss. Cones were ablated with ultrafast laser delivered through adaptive optics scanning...
e13061 Background: Fulvestrant, a selective estrogen receptor (ER) down-regulator, is standard of care for first-line treatment in hormone receptor-positive (HR+) advanced breast cancer. This study to assess the safety profile and effectiveness fulvestrant 500mg as endocrine therapy post-menopausal women with HR+ locally or metastatic Methods: The an open-label, multicentre, prospective, observational China. Chinese patients receiving first agent after relapse according investigator’s...
Though the responses of rich variety retinal ganglion cells (RGCs) reflect totality visual processing in retina and provide sole conduit for those processed to brain, we have much learn about how brain uses these signals guide behavior. An impediment developing a comprehensive understanding role circuits behavior is paucity causal studies intact primate system. Here demonstrate ability optogenetically activate individual RGCs with flashes light focused on single RGC somas vivo , without...
Though the responses of rich variety retinal ganglion cells (RGCs) reflect totality visual processing in retina and provide sole conduit for those processed to brain, we have much learn about how brain uses these signals guide behavior. An impediment developing a comprehensive understanding role circuits behavior is paucity causal studies intact primate system. Here demonstrate ability optogenetically activate individual RGCs with flashes light focused on single RGC somas vivo , without...
Abstract In this study, We comprehensively analyzed 20 genes from The Cancer Genome Atlas(TCGA) HNSCC database (YTHDF1, FTO, YTHDF2, YTHDF3, HNRNPA2B1, METTL14, METTL3, IGF2BP1, HNRNPC, IGF2BP2, RBMX, IGF2BP3, YTHDC1, YTHDC2, RBM15, RBM15B, VIRMA, ZC3H13, WTAP, ALKBH5) to predict whether there is an impact on the prognosis of patients with HNSCC. importance m6A regulators such as VIRMA in pathogenesis was emphasized. These findings will provide a therapeutic approach and new research...