A5101454457- Osteoarthritis Treatment and Mechanisms
- dental development and anomalies
- Bone and Dental Protein Studies
- Periodontal Regeneration and Treatments
- Orthodontics and Dentofacial Orthopedics
- Bone Metabolism and Diseases
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Oral and Maxillofacial Pathology
- 3D Printing in Biomedical Research
- Bone Tissue Engineering Materials
- Cell Adhesion Molecules Research
- Bone health and treatments
- Connective tissue disorders research
- Temporomandibular Joint Disorders
- Corneal Surgery and Treatments
- Tendon Structure and Treatment
- Cellular Mechanics and Interactions
- Molecular Biology Techniques and Applications
- Single-cell and spatial transcriptomics
- Mechanical Behavior of Composites
- Dental Implant Techniques and Outcomes
- Proteoglycans and glycosaminoglycans research
- Immune Cell Function and Interaction
- Tissue Engineering and Regenerative Medicine
Suizhou Central Hospital
2025
PetroChina Southwest Oil and Gas Field Company (China)
2024
Texas A&M University
2015-2023
Academy of Military Medical Sciences
2019-2021
Stomatology Hospital
2015-2021
Guangzhou Medical University
2021
Gaston College
2017-2018
Qufu Normal University
2017
Second Affiliated Hospital of Xi'an Jiaotong University
2016
Nanjing Drum Tower Hospital
2016
A comprehensive understanding of the cellular heterogeneity and molecular mechanisms underlying development, homeostasis, disease human intervertebral disks (IVDs) remains challenging. Here, transcriptomic landscape 108 IVD cells was mapped using single-cell RNA sequencing three main compartments from young adult healthy IVDs, including nucleus pulposus (NP), annulus fibrosus, cartilage endplate (CEP). The chondrocyte subclusters were classified based on their potential regulatory,...
For decades, it has been widely accepted that hypertrophic chondrocytes undergo apoptosis prior to endochondral bone formation. However, very recent studies in long suggest can directly transform into cells. Our initial vivo characterization of condylar revealed modest numbers apoptotic cells but high levels antiapoptotic Bcl-2 expression, some dividing cells, and clear alkaline phosphatase activity (early marker). Ex culture newborn cartilage on a chick chorioallantoic membrane showed after...
Human skeletal stem cells (SSCs) have been discovered in fetal and adult long bones. However, the spatiotemporal ontogeny of human embryonic SSCs during early skeletogenesis remains elusive. Here we map transcriptional landscape limb buds bones at single-cell resolution to address this fundamental question. We found remarkable heterogeneity within bud mesenchyme epithelium, aligned them along proximal-distal anterior-posterior axes using known marker genes. Osteo-chondrogenic progenitors...
To test the null hypothesis that there is no effect of maxillary incisor labiolingual inclination and anteroposterior (AP) position on smiling profile esthetics in young adult females.A facial photograph a Chinese woman with normal profile, Class I occlusion, skeletal pattern was digitized. The digital image modified to obtain four series comprising 29 profiles. sample individuals rating these pictures comprised 21 orthodontic professionals 66 undergraduates. Post hoc tests were done Student...
Although chondrogenesis and osteogenesis are considered as two separate processes during endochondral bone formation after birth, recent studies have demonstrated the direct cell transformation from chondrocytes into cells in postnatal growth. Here we use lineage tracing multiple vivo approaches to study role of Bmpr1a endochondrogenesis. Our data showed profound changes skeletal shape, size structure when was deleted using Aggrecan-Cre ERT2 early cartilage with a one-time tamoxifen...
The integration of controlled growth factor delivery and biomimetic architecture into a microsphere is challenging but attractive strategy for developing new injectable biomaterials. In this work, unique hierarchical nanosphere‐encapsulated‐in‐microsphere scaffolding system developed. First, heparin‐conjugated gelatin (HG) synthesized, which provides binding domains bone morphogenetic protein 2 (BMP2) to stabilize factor, protect it from denaturation proteolytic degradation, subsequently...
An injectable composite scaffold incorporating decellularized adipose tissue (DAT) as a bioactive matrix within hydrogel phase capable of in situ polymerization would be advantageous for adipose-derived stem cell (ASC) delivery the filling small or irregular soft defects. Building on previous work, current study investigates DAT milling methods and effects particle size seeding density response human ASCs encapsulated photo-cross-linkable methacrylated chondroitin sulphate (MCS)—DAT...
Abstract Whereas the critical roles of innate lymphoid cells (ILCs) in adult are increasingly appreciated, their developmental hierarchy early human fetus remains largely elusive. In this study, we sorted hematopoietic stem/progenitor cells, progenitors, putative ILC progenitor/precursors and mature ILCs fetal hematopoietic, non-lymphoid tissues, from 8 to 12 post-conception weeks, for single-cell RNA-sequencing, followed by computational analysis functional validation at bulk levels. We...
Wnt–β-catenin signaling plays a key role in orthodontic tooth movement (OTM), common clinical practice for malocclusion correction. However, its targeted periodontal ligament (PDL) progenitor cells remain largely unclear. In this study, we first showed synchronized increase levels and Axin2 + PDL cell numbers during OTM using immunostaining of β-catenin wild-type mice X-gal staining the Axin2-LacZ knock-in line. Next, demonstrated time-dependent increases progenitors their progeny within...
Background/Objectives: Nestin is an intermediate filament protein and a marker of odontoblasts, but its function in tooth bone formation largely unknown. This study aimed to determine whether plays role postnatal formation. Methods: 4-week-old knockout (KO) mice were analyzed with range techniques, including X-ray imaging, uCT, backscattered acid-etched casted SEM, FITC-confocal microscopy, H&E TRAP staining, immunohistochemistry. Results: The KO had no apparent difference crown compared...
Recent studies have revealed multiple roles of osteocytes in bone metabolism. However, detailed analyses the embedded structure are still limited because high mineral content around these cells. In this study, we developed an innovative technique, "FITC-Imaris technique", which combines FITC ([2, 5]-Fluorescein isothiocyanate), confocal microscopy and Imaris software. With method, could not only visualize 3-D morphology osteocytes, but more importantly, were able to statistically quantitate...
Bone-forming osteoblasts have been a cornerstone of bone biology for more than century.Most research toward and diseases center on osteoblasts.Overlooked are the 90% cells, called osteocytes.This study aims to test hypothesis that osteocytes but not directly build mineralized structures, defects in lead onset hypophosphatemia rickets.The was tested by developing modifying multiple imaging techniques, including both vivo vitro models plus two types rickets (Dmp1-null Hyp, Phex mutation mice),...
Osterix (Osx) is a transcription factor essential for osteoblast differentiation and bone mineralization. Although there are indications that Osx also plays regulatory role in cartilage, this has not been well-studied. The goal of study was to define the function post-natal growth secondary cartilage at mandibular condyle. Conditional knockout (cKO) mice were missing only generated by crossing Osx-loxP Aggrecan-Cre mice. Cre activity induced tamoxifen injection twice week from day 12 1 mo...
Aim: The process of orthodontic tooth movement (OTM) involves multiple mechanisms action including bone and extracellular matrix remodelling, although the role periodontal ligament (PDL) in this is largely unknown. Periostin, which highly expressed PDL, known to be responsible for mechanical stimulation maintaining integrity tissues. We hypothesize that protein plays an important during OTM.
A recent breakthrough showing that direct trans-differentiation of chondrocytes into bone cells commonly occurs during endochondral formation in the growth plate, articular cartilage, and mandibular condylar cartilage suggests chondrogenesis osteogenesis are likely one continuous biological process instead two separate processes. Yet, gene regulation this cell transformation is largely unclear. Here, we employed cartilage-specific β-catenin loss-of-function (β-catenin fx/fx )...
An electrospun fibrous scaffold was fabricated and used in the<italic>in situ</italic>remediation of rabbits’ corneal stromata.