A5079207349- Research on Leishmaniasis Studies
- Parasites and Host Interactions
- Trypanosoma species research and implications
- Redox biology and oxidative stress
- Glutathione Transferases and Polymorphisms
- Trace Elements in Health
- Sulfur Compounds in Biology
- Parasite Biology and Host Interactions
- Parasitic infections in humans and animals
- Selenium in Biological Systems
- Microbial bioremediation and biosurfactants
- Biochemical and Molecular Research
- Metalloenzymes and iron-sulfur proteins
- Social Sciences and Policies
- S100 Proteins and Annexins
- Genomics, phytochemicals, and oxidative stress
- Biofuel production and bioconversion
- Enzyme function and inhibition
- Sulfur-Based Synthesis Techniques
- Metal-Catalyzed Oxygenation Mechanisms
- Amino Acid Enzymes and Metabolism
- Heat shock proteins research
- Microbial Metabolic Engineering and Bioproduction
- Environmental Chemistry and Analysis
- Neonatal Respiratory Health Research
Institut Pasteur de Montevideo
2015-2025
Universidad Santiago de Cali
2024
Universidad de la República
2008-2020
Institut Pasteur
2015
Centro Uruguayo de Imagenología Molecular
2013
University of Florida
2002
Platyhelminth parasites are a major health problem in developing countries. In contrast to their mammalian hosts, platyhelminth thiol-disulfide redox homeostasis relies on linked thioredoxin-glutathione systems, which fully dependent reductase (TGR), promising drug target. TGR is homodimeric enzyme comprising glutaredoxin domain and thioredoxin (TR) domains with C-terminal center containing selenocysteine (Sec). this study, we demonstrate the existence of functional systems cytosolic...
Journal Article Production and characterization of a new bioemulsifier from Pseudomonas putida ML2 Get access M. Bonilla, Bonilla Departments Microbiology Search for other works by this author on: Oxford Academic Google Scholar C. Olivaro, Olivaro Pharmacognosy Natural Products, School Chemistry, Universidad de la República, Montevideo, Uruguay Corona, Corona A. Vazquez, Vazquez Soubes Matilde Soubes, Department Microbiology, Gral. Flores 2124, Casilla Correo 1157, (e‐mail:...
Abstract Assisted reproductive techniques are routinely used in livestock species to increase and enhance productivity. Ovarian hyperstimulation is a process that currently relies on administering pituitary‐derived follicle‐stimulating hormone (FSH) or equine chorionic gonadotropin combination with other hormones promote the maturation of multiple follicles thereby achieve superovulation. The use partially purified preparations FSH extracted from natural sources associated suboptimal...
Parasitic flatworms are responsible for serious infectious diseases that affect humans as well livestock animals in vast regions of the world. Yet, drug armamentarium available treatment these infections is limited: praziquantel single currently 200 million people infected with Schistosoma spp. and there justified concern about emergence resistance. Thioredoxin glutathione reductase (TGR) an essential core enzyme redox homeostasis flatworm parasites. In this work, we searched TGR inhibitors...
In most organisms, thioredoxin (Trx) and/or glutathione (GSH) systems are essential for redox homeostasis and deoxyribonucleotide synthesis. Platyhelminth parasites have a unique simplified thiol-based system, in which the selenoprotein thioredoxin-glutathione reductase (TGR), fusion of glutaredoxin (Grx) domain to canonical domains, is sole enzyme supplying electrons oxidized (GSSG) Trx. This has recently been validated as key drug target flatworm infections. this study, we show that TGR...
Abstract Background The thioredoxin and/or glutathione pathways occur in all organisms. They provide electrons for deoxyribonucleotide synthesis, function as antioxidant defenses, detoxification, Fe/S biogenesis and participate a variety of cellular processes. In contrast to their mammalian hosts, platyhelminth (flatworm) parasites studied so far, lack conventional systems. Instead, they possess linked thioredoxin-glutathione system with the selenocysteine-containing enzyme reductase (TGR)...
African trypanosomiasis is a major problem for human and animal health in endemic countries, where it threatens millions of people affects economic development. New drugs are needed to overcome the toxicity, administration, low efficacy, resistance issues current chemotherapy. Robust, simple, economical high-throughput, whole-cell-based assays required accelerate identification novel chemical entities. With this aim, we generated bioluminescent cell line bloodstream stage Trypanosoma brucei...
New drugs are needed to treat flatworm infections that cause severe human diseases such as schistosomiasis. The unique enzyme thioredoxin glutathione reductase (TGR), structurally different from the enzyme, is a key drug target. Structural studies of Echinococcus granulosus TGR, free and complexed with Au
Trypanosoma brucei glutaredoxin 2 (Grx2) is a dithiol that specifically located in the mitochondrial intermembrane space. Bloodstream form parasites lacking Grx2 or both, and cytosolic Grx1, are viable vitro infectious to mice suggesting neither oxidoreductase needed for survival infectivity mammals. A 37 °C 39 shift changes cellular redox milieu of bloodstream cells more oxidizing conditions induces significantly stronger growth arrest wildtype compared mutant cells. Grx2-deficient...
Most known thioredoxin-type proteins (Trx) participate in redox pathways, using two highly conserved cysteine residues to catalyze thiol-disulfide exchange reactions. Here we demonstrate that the so far unexplored Trx2 from African trypanosomes (Trypanosoma brucei) lacks protein disulfide reductase activity but functions as an effective temperature-activated and redox-regulated chaperone. Immunofluorescence microscopy fractionated cell lysis revealed is located mitochondrion of parasite....
SUMMARY Infection by larval Echinococcus granulosus is usually characterized tight inflammatory control. However, various degrees of chronic granulomatous inflammation are also observed, reaching a high point in infection cattle the most prevalent parasite strain worldwide, which not well adapted to this host species. In context, epithelioid and multinucleated giant macrophages surround parasite, secreted products these cells often associate with wall. The phagocyte-specific S100 proteins,...
Aims: Members of the thioredoxin (Trx) protein family participate mainly in redox pathways and have not been associated with Fe/S binding, contrast to some closely related glutaredoxins (Grxs). Cestode parasites possess an unusual diversity Trxs Trx-related proteins unexplored functions. In this study, we addressed biochemical characterization a new class (IsTRP) classical monothiol Grx (EgGrx5) from human pathogen Echinococcus granulosus. Results: The dimeric form IsTRP coordinates Fe2S2...
Parasitic flatworms cause serious infectious diseases that affect humans and livestock in vast regions of the world, yet there are few effective drugs to treat them. Thioredoxin glutathione reductase (TGR) is an essential enzyme for redox homeostasis flatworm parasites a promising pharmacological target. We purified homogeneity characterized TGR from tapeworm Mesocestoides vogae (syn. M. corti). This purification revealed absence conventional TR GR. The activity exhibits hysteretic behavior...
Glutaredoxins (Grx) are small proteins conserved throughout all the kingdoms of life that engaged in a wide variety biological processes and share common thioredoxin-fold. Among them, class II Grx redox-inactive involved iron-sulfur (FeS) metabolism. They contain single thiol group their active site use low molecular mass thiols such as glutathione ligand for binding FeS-clusters. In this study, we investigated aspects 1CGrx1 from pathogenic parasite Trypanosoma brucei brucei, mitochondrial...
El presente trabajo inicia otorgando una breve descripción tanto de la justicia restaurativa como reinserción social, siendo necesario analizar en medida lo posible sus orígenes, características, objetivos y elementos que pueden ayudar a cualquier persona entenderlos. Con esto se buscó enfatizar importancia dichos conceptos, no solo sociedad ecuatoriana, sino toda Latinoamérica e incluso varias partes del mundo, donde pesar los esfuerzos tomados por entidades gubernamentales, particulares;...