A5088703357- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Advanced Sensor and Energy Harvesting Materials
- Hippo pathway signaling and YAP/TAZ
- Hydrogels: synthesis, properties, applications
- Micro and Nano Robotics
- Protein Degradation and Inhibitors
- Nuclear Structure and Function
- Planarian Biology and Electrostimulation
- Gut microbiota and health
- Conducting polymers and applications
- Aortic Disease and Treatment Approaches
- Polymer Surface Interaction Studies
- Dendrimers and Hyperbranched Polymers
- Advanced Polymer Synthesis and Characterization
- biodegradable polymer synthesis and properties
- Additive Manufacturing and 3D Printing Technologies
- Cardiac Valve Diseases and Treatments
- Infective Endocarditis Diagnosis and Management
- Digestive system and related health
- Cellular transport and secretion
- Liver physiology and pathology
- Skin and Cellular Biology Research
- Microfluidic and Bio-sensing Technologies
University of Colorado Boulder
2018-2024
McMaster University
2014
Epithelial organoids are stem cell–derived tissues that approximate aspects of real organs, and thus they have potential as powerful tools in basic translational research. By definition, self-organize, but the structures formed often heterogeneous irreproducible, which limits their use lab clinic. We describe methodologies for spatially temporally controlling organoid formation, thereby rendering a stochastic process more deterministic. Bioengineered cell microenvironments used to specify...
Spatiotemporally coordinated transformations in epithelial curvature are necessary to generate crypt-villus structures during intestinal development. However, the temporal regulation of mechanotransduction pathways that drive crypt morphogenesis remains understudied. Intestinal organoids have proven useful study vitro, yet reliance on static culture scaffolds limits ability assess effects changing curvature. Here, a photoinduced hydrogel cross-link exchange reaction is used spatiotemporally...
Abstract Intestinal organoid protocols rely on the use of extracellular scaffolds, typically Matrigel, and upon switching from growth to differentiation promoting media, a symmetry breaking event takes place. During this stage, first bud like structures analogous crypts protrude central body ensues. While organoids provide unparalleled architectural functional complexity, sophistication is also responsible for high variability lack reproducibility uniform crypt‐villus structures. If function...
Abstract Intestinal organoids are useful in vitro models for basic and translational studies aimed at understanding treating disease. However, their routine culture relies on animal‐derived matrices that limit translation to clinical applications. In fact, there few fully defined, synthetic hydrogel systems allow the expansion of intestinal organoids. Here, an allyl sulfide photodegradable is presented, achieving rapid degradation through radical addition‐fragmentation chain transfer (AFCT)...
Stiffness and forces are two fundamental quantities essential to living cells tissues. However, it has been a challenge quantify both 3D traction stiffness (or modulus) using the same probe in vivo. Here, we describe an approach that overcomes this by creating magnetic microrobot with controllable functionality. Biocompatible ferromagnetic cobalt-platinum microcrosses were fabricated, each microcross (about 30 micrometers) was trapped inside arginine–glycine–aspartic acid–conjugated stiff...
Muscle cells sense the mechanical properties of their microenvironment, and these can change in response to injury or disease. Hydrogels with dynamic material be used study effect such varying signals. Here, we report ability azadibenzocyclooctyne undergo a cytocompatible, photoinitiated crosslinking reaction. This reaction is exploited as strategy for on-demand stiffening three-dimensional cell scaffolds formed through an initial strain-promoted azide-alkyne cycloaddition. Myoblasts...
Objective: Resident valvular interstitial cells (VICs) activate to myofibroblasts during aortic valve stenosis progression, which further promotes fibrosis or even differentiate into osteoblast-like that can lead calcification of tissue. Inflammation is a hallmark stenosis, so we aimed determine proinflammatory cytokines secreted from M1 macrophages give rise transient VIC phenotype leads Approach and Results: We designed hydrogel biomaterials as extracellular matrix mimics enabling the...
3D organoid models have recently seen a boom in popularity, as they can better recapitulate the complexity of multicellular organs compared to other vitro culture systems. However, organoids are difficult image because limited penetration depth high-resolution microscopes and depth-dependent light attenuation, which limit understanding signal transduction pathways characterization intimate cell-extracellular matrix (ECM) interactions. To overcome these challenges, phototransfer by allyl...
Abstract Biofabrication allows for the templating of structural features in materials on cellularly-relevant size scales, enabling generation tissue-like structures with controlled form and function. This is particularly relevant growing organoids, where application biochemical biomechanical stimuli can be used to guide assembly differentiation stem cells architectures similar parent tissue or organ. Recently, ablative laser-scanning techniques was create 3D overhang collagen hydrogels at...
Injectable, in situ-gelling nanostructured hydrogels have been prepared from hydrazide and aldehyde-functionalized polymer precursors based on a copolymer of oligo(ethylene glycol) methacrylate (OEGMA) an oligo(lactic acid) macromonomer (OLA) with varying lactic acid chain lengths. The resulting contain mix chemical (hydrazone bond formation between aldehyde groups) physical (hydrophobic interactions OLA chains) cross-links which form competitively as function the length density. An increase...
Initial landmark studies in the design of synthetic hydrogels for intestinal organoid culture identified precise matrix requirements differentiation, namely decompression matrix-imposed forces and supplementation laminin. But beyond stating necessity laminin, organoid-laminin interactions have gone largely unstudied, as this ubiquitous requirement exogenous laminin hinders investigation. In work, we exploit a fast stress relaxing, boronate ester based hydrogel organoids, fortuitously...
While injectable, in situ gelling hydrogels have attracted increasing attention the biomedical literature due to their minimally invasive administration potential, little is known about internal morphology of these and thus how engineer precursor polymer compositions achieve desired hydrogel properties. In this paper, injectable based on hydrazide aldehyde-functionalized poly(oligoethylene glycol methacrylate) precursors with varying lower critical solution temperatures (LCSTs) investigated...
Dynamic windows based on reversible metal electrodeposition are an attractive way to enhance the energy efficiency of buildings and show great commercial potential. that rely liquid electrolytes at risk short circuiting when two electrodes contact, especially larger-scale. Here we developed a poly (vinyl alcohol) (PVA) gel polymer electrolyte (GPE) with 85% transmittance, is, sufficiently stiff act as separator. The GPE is implemented into exhibit comparable electrochemical optical...
The cell nucleus is continuously exposed to external signals, of both chemical and mechanical nature. To ensure proper cellular response, cells need regulate not only the transmission these but also their timing duration. Such timescale regulation well described for fluctuating if how it applies signals reaching still unknown. Here we demonstrate that formation fibrillar adhesions locks in a mechanically deformed conformation, setting response adhesion remodelling (~1 hour). This process...
This study describes the synthesis of novel amphiphilic linear-dendritic block copolymers and their self-assembly in water to form supramolecular nanoreactors capable catalyzing Suzuki-Miyaura coupling reactions under “green” conditions. The were formed through copper(I)-catalyzed alkyne-azide cycloaddition between azide functionalized poly(benzyl ether) dendrons as perfectly branched blocks, well bis-alkyne modified poly(ethylene glycol), PEG, linear block. A first-generation dendron (G1)...
In article number 1901214 by Kristi S. Anseth and co-workers, intestinal organoids are grown in synthetic hydrogels with photodegradable, allyl sulfide linkers. These materials used to determine the effects of matrix softening on size resulting crypts. The image shows a differentiated organoid immunofluorescent staining for F-actin (green), nuclei (blue) paneth cells (red), marker crypt formation.