A5060013866- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Force Microscopy Techniques and Applications
- Cellular and Composite Structures
- Microfluidic and Bio-sensing Technologies
- Transportation Safety and Impact Analysis
- Micro and Nano Robotics
- RNA Research and Splicing
- Automotive and Human Injury Biomechanics
- Spectroscopy Techniques in Biomedical and Chemical Research
- Nuclear Structure and Function
- Cancer Cells and Metastasis
- Genomics and Chromatin Dynamics
- Pluripotent Stem Cells Research
- Pancreatic and Hepatic Oncology Research
- High-Velocity Impact and Material Behavior
- Spectroscopy and Chemometric Analyses
- AI in cancer detection
- Cell Image Analysis Techniques
- Cell Adhesion Molecules Research
- Advanced Biosensing Techniques and Applications
- Spaceflight effects on biology
- Mineral Processing and Grinding
- Biosensors and Analytical Detection
- Wireless Body Area Networks
Southern Illinois University Carbondale
2016-2023
University of Illinois Urbana-Champaign
2021-2023
Stiffness and forces are two fundamental quantities essential to living cells tissues. However, it has been a challenge quantify both 3D traction stiffness (or modulus) using the same probe in vivo. Here, we describe an approach that overcomes this by creating magnetic microrobot with controllable functionality. Biocompatible ferromagnetic cobalt-platinum microcrosses were fabricated, each microcross (about 30 micrometers) was trapped inside arginine–glycine–aspartic acid–conjugated stiff...
Desktop wax printer was employed to build high throughput platforms for studying stem cells fate.
A machine learning approach is applied to Raman spectra of cells from the MIA PaCa-2 human pancreatic cancer cell line distinguish between tumor repopulating (TRCs) and parental control cells, aid in identification molecular signatures. Fifty-one two types are analyzed determine best combination data type, dimension size, classification technique differentiate types. An accuracy 0.98 obtained support vector (SVM) k-nearest neighbor (kNN) classifiers with various reduction feature selection...
Soft 3D-fibrin-gel selected tumor repopulating cells (TRCs) from the B16F1 melanoma cell line exhibit extraordinary self-renewal and tumor-regeneration capabilities. However, their biomarkers gene regulatory features remain largely unknown. Here, we utilized next-generation sequencing-based RNA sequencing (RNA-seq) technique to discover novel active of TRCs. Systems biology analysis RNA-seq data identified differentially expressed clusters, including adhesion cluster, which subsequently...
Polymerized polyacrylamide (PAA) substrates are linearly elastic hydrogels that widely used in mechanosensing studies due to their biocompatibility, wide range of functionalization capability, and tunable mechanical properties. However, such cellular response on purely substrates, which do not mimic the viscoelastic living tissues, may be physiologically relevant. Because 2D PAA remains largely unknown, we stereolithography (SLA)-based additive manufacturing technique create with properties...
Mechanoregulation of cell–extracellular matrix (ECM) interactions are crucial for dictating pluripotent stem cell differentiation. However, not all cells respond homogeneously which results in heterogeneous populations. When cells, such as mouse epiblast (EpiSCs), cultured clusters, the heterogeneity effect during differentiation is even more pronounced. While past studies implicated variations signaling pathways to be root cause heterogeneity, biophysical aspects have been thoroughly...