A5059983558- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Protein Structure and Dynamics
- Fibroblast Growth Factor Research
- Chemical Reactions and Isotopes
- Tendon Structure and Treatment
- RNA Research and Splicing
- Protein Tyrosine Phosphatases
- Prion Diseases and Protein Misfolding
- Advanced Breast Cancer Therapies
- Alzheimer's disease research and treatments
- Genomics and Chromatin Dynamics
- Photoreceptor and optogenetics research
- Force Microscopy Techniques and Applications
- Sphingolipid Metabolism and Signaling
- Circadian rhythm and melatonin
- Wound Healing and Treatments
- Synthesis and Characterization of Heterocyclic Compounds
- Nuclear Structure and Function
- Planarian Biology and Electrostimulation
University of Illinois Urbana-Champaign
2016-2021
University of Arizona
2013-2016
At the onset of collective cell migration, a subset cells within an initially homogenous population acquires distinct 'leader' phenotype with characteristic morphology and motility. However, factors driving leader formation as well mechanisms regulating density during migration process remain to be determined. Here we use single-cell gene expression analysis computational modelling show that identity is dynamically regulated by Dll4 signalling through both Notch1 cellular stress in migrating...
Increasing evidence shows that mechanical stresses are critical in regulating cell functions, fate, and diseases. However, no methods exist can quantify isotropic compressive stresses. Here we describe fluorescent nanoparticle-labeled, monodisperse elastic microspheres made of Arg-Gly-Asp-conjugated alginate hydrogels (elastic round microgels, ERMGs). We generate 3D displacements calculate strains tractions exerted on an ERMG. Average ERMG 570 Pa within layers 360 tumor-repopulating (TRC)...
Mechanical forces play important roles in development, physiology, and diseases, but how force is transduced into gene transcription remains elusive. Here, we show that of transgene DHFR or endogenous genes egr-1 Cav1 rapidly up-regulated response to cyclic applied via integrins at low frequencies not 100 Hz. Gene up-regulation does follow the weak power law with frequency. Force-induced nuclear interior associated demethylation histone H3 lysine-9 trimethylation (H3K9me3), whereas no near...
The kinetics of amyloid fibril formation by beta-amyloid peptide (Abeta) are typical a nucleation-dependent polymerization mechanism. This type mechanism suggests that the study interaction Abeta with itself can provide some valuable insights into Alzheimer disease amyloidosis. Interaction was explored yeast two-hybrid system. Fusion proteins were created linking fragment to LexA DNA-binding domain (bait) and also B42 transactivation (prey). Protein-protein interactions measured expression...
A key feature of multicellular systems is the ability cells to function collectively in response external stimuli. However, mechanisms intercellular cell signaling and their functional implications diverse vascular structures are poorly understood. Using a combination computational modeling plasma lithography micropatterning, we investigate roles structural arrangement endothelial collective calcium contractility. Under histamine stimulation, self-assembled microengineered networks, but not...
Previous studies have demonstrated that integrin-linked kinases (ILKs) are abundantly expressed in extracellular matrix (ECM) riche dermis, hair follicles, and basal cells of epidermis. ILKs not only essential for the maintenance skin structure, but also play important roles wound healing. can promote formation granulation tissue by stimulating proliferation fibroblasts secretion ECM, accelerate contraction inducing differentiation to myofibroblasts, boost reepithelization promoting...