A5014512379- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Labor Movements and Unions
- Fungal and yeast genetics research
- International Labor and Employment Law
- Enzyme Catalysis and Immobilization
- Catalysis for Biomass Conversion
- Microbial Metabolites in Food Biotechnology
- Viral Infectious Diseases and Gene Expression in Insects
- Global trade, sustainability, and social impact
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacteriophages and microbial interactions
- Insect Resistance and Genetics
- Cultural Heritage Management and Preservation
- Water Quality and Pollution Assessment
- Insect and Pesticide Research
- Genomics and Phylogenetic Studies
- Phytase and its Applications
- Anaerobic Digestion and Biogas Production
- Studies on Chitinases and Chitosanases
- Alzheimer's disease research and treatments
- Water Treatment and Disinfection
- Prion Diseases and Protein Misfolding
- Asian Industrial and Economic Development
Center for Food Safety and Applied Nutrition
2024
United States Food and Drug Administration
2024
Applied DNA Sciences (United States)
2020
Agricultural Research Service
2008-2017
United States Department of Agriculture
2008-2017
National Center for Agricultural Utilization Research
2008-2017
University of Leeds
2012-2016
Newcastle University
2003-2013
Royal Commission on the Ancient and Historical Monuments of Wales
1994-2010
Freeman Hospital
2008
We have used the yeast two-hybrid system to isolate cDNAs encoding proteins that specifically interact with 42-aa β-amyloid peptide (Aβ), a major constituent of senile plaques in Alzheimer’s disease. The carboxy terminus α 2 -macroglobulin (α2M), proteinase inhibitor released response inflammatory stimuli, was identified as strong and specific interactor Aβ, utilizing this system. Direct evidence for interaction obtained by co-immunoprecipitation α2M Aβ from cell, formation SDS-resistant...
Research addressing the occurrence, fate and effects of pharmaceuticals in aquatic environment has expanded rapidly over past two decades, primarily due to development improved chemical analysis methods. Significant research gaps still remain, however, including a lack longer term, repeated monitoring rivers, determination temporal spatial changes pharmaceutical concentrations, inputs from sources other than wastewater treatment plants (WWTPs), such as combined sewer overflows (CSOs). In...
Acetone, butanol, and ethanol (ABE) were produced from corn fiber arabinoxylan (CFAX) CFAX sugars (glucose, xylose, galactose, arabinose) using Clostridium acetobutylicum P260. In mixed sugar fermentation, the culture preferred glucose arabinose over galactose xylose. Under experimental conditions, (60 g/L) was not fermented until either 5 g/L xylose or plus xylanase enzyme added to support initial growth fermentation. this system, C. 9.60 ABE This experiment resulted in a yield productivity...
Reduced yields of ethanol due to bacterial contamination in fermentation cultures weaken the economics biofuel production. Lactic acid bacteria are considered most problematic, and surveys commercial fuel facilities have found that species Lactobacillus predominant. Bacteriophage lytic enzymes peptidoglycan hydrolases can degrade Gram positive cell wall when exposed externally provide a novel source antimicrobials highly refractory resistance development.The streptococcal phage LambdaSa2...
One of the challenges facing fuel ethanol industry is management bacterial contamination during fermentation. Lactobacillus species are predominant contaminants that decrease profitability biofuel production by reducing yields and causing "stuck" fermentations, which incur additional economic losses via expensive antibiotic treatments disinfection costs. The current use has led to emergence drug-resistant strains, residues in distillers dried grains with solubles (DDGS) a concern for feed...
Two recent outbreaks of listeriosis have been linked to the consumption enoki mushrooms. After first outbreak, import sampling by U.S. FDA identified that 43% samples evaluated were positive for Listeria monocytogenes (Lm). These observations raised questions about potential sources Lm contamination One source is during mushroom cultivation, as growing conditions are comparatively cool and moist induce germination, which well adapted. varieties mushrooms determine contaminate cultures when...
Saccharomyces strains engineered to ferment xylose using Scheffersomyces stipitis reductase (XR) and xylitol dehydrogenase (XDH) genes appear be limited by metabolic imbalances, due differing cofactor specificities of XR XDH. The S. XR, which uses both NADH NADPH, is hypothesized reduce the imbalance, allowing fermentation in this yeast. However, unadapted cerevisiae expressing grow poorly on xylose, suggesting that metabolism still imbalanced, even under aerobic conditions. In study, we...
Scheffersomyces (formerly Pichia) stipitis NRRL Y-7124 was mutagenized using UV-C irradiation to produce yeast strains for anaerobic conversion of lignocellulosic sugars ethanol. potentially produces large numbers random mutations broadly and uniformly over the whole genome generate unique strains. Wild-type cultures S. were subjected (234 nm) targeted at approximately 40% cell survival. When surviving cells selected in sufficient via automated plating strategies cultured anaerobically on...
The kinetics of amyloid fibril formation by beta-amyloid peptide (Abeta) are typical a nucleation-dependent polymerization mechanism. This type mechanism suggests that the study interaction Abeta with itself can provide some valuable insights into Alzheimer disease amyloidosis. Interaction was explored yeast two-hybrid system. Fusion proteins were created linking fragment to LexA DNA-binding domain (bait) and also B42 transactivation (prey). Protein-protein interactions measured expression...