A5101599675- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Enzyme Production and Characterization
- Advanced Sensor Technologies Research
- Microbial Metabolites in Food Biotechnology
- Fungal and yeast genetics research
- Scientific Measurement and Uncertainty Evaluation
- Asphalt Pavement Performance Evaluation
- Catalysis for Biomass Conversion
- Probiotics and Fermented Foods
- Infrastructure Maintenance and Monitoring
- Advanced MEMS and NEMS Technologies
- Spacecraft Design and Technology
- Magnetic Bearings and Levitation Dynamics
- Flow Measurement and Analysis
- Minerals Flotation and Separation Techniques
- Magnetic and Electromagnetic Effects
- Turbomachinery Performance and Optimization
- Experimental and Theoretical Physics Studies
- Soil and Unsaturated Flow
- Power Systems and Technologies
- Metal Extraction and Bioleaching
- Inertial Sensor and Navigation
- Transport Systems and Technology
Shanghai Jiao Tong University
2020-2022
National Center for Agricultural Utilization Research
2009-2019
Agricultural Research Service
2007-2018
United States Department of Agriculture
2007-2018
Wuhan University of Technology
2018
Kunming University of Science and Technology
2012-2016
China Nonferrous Metal Mining (China)
2015
Chongqing Technology and Business University
2005
University of Florida
2005
Rogers (United States)
2005
The emergence of antibiotic-resistant bacteria may limit the effectiveness antibiotics to treat bacterial contamination in fuel ethanol plants, and therefore, new antibacterial intervention methods tools test their application are needed. Using shake-flask cultures Saccharomyces cerevisiae grown on saccharified corn mash strains lactic acid isolated from a dry-grind facility, simple model simulate infection was developed. Challenging with 10(8) CFU/mL Lactobacillus fermentum decreased yield...
High temperature measurement of the rotating parts in aero-engine system requires wireless passive sensors. Surface acoustic wave (SAW) devices capable measuring high wirelessly are therefore ideal for these extreme scenarios where wired sensors not applicable. In this article, SAW built on LiNbO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> substrate which can measure up to 1100 °C with a good repeatability and endurance reported. The...
Scheffersomyces (formerly Pichia) stipitis NRRL Y-7124 was mutagenized using UV-C irradiation to produce yeast strains for anaerobic conversion of lignocellulosic sugars ethanol. potentially produces large numbers random mutations broadly and uniformly over the whole genome generate unique strains. Wild-type cultures S. were subjected (234 nm) targeted at approximately 40% cell survival. When surviving cells selected in sufficient via automated plating strategies cultured anaerobically on...
Lactobacillus buchneri strain NRRL B-30929 was a contaminant obtained from commercial ethanol fermentation. This facultative anaerobe is unique because of its rapid growth on xylose and simultaneous fermentation glucose. The utilizes broad range carbohydrate substrates possesses high tolerance to other stresses, making it an attractive candidate for bioconversion biomass various bioproducts. genome sequence will provide insight into the properties this lactic acid bacterium.
Economically important plants contain large amounts of inulin. Disposal waste resulting from their processing presents environmental issues. Finding microorganisms capable converting inulin to biofuel and valuable co-products at the site would have significant economic impact. We evaluated ability two mutant strains Kluyveromyces marxianus (Km7 Km8) utilize for ethanol production. In glucose medium, both consumed all produced 0.40 g ethanol/g 24 h. Km7 exhibited maximum colony forming units...
A total of 33 Lactobacillus strains were screened for feruloyl esterase (FE) activity using agar plates containing ethyl ferulate as the sole carbon source, and fermentum NRRL B-1932 demonstrated strongest FE among a dozen species showing clearing zone on opaque plate ferulate. activities monitored high-performance liquid chromatography with an acetonitrile-trifluoroacetic acid gradient. To produce sufficient purified from L. strain (LfFE), cDNA encoding LfFE (Lffae) was amplified cloned by...
Engineering the industrial ethanologen Saccharomyces cerevisiae to use pentose sugars from lignocellulosic biomass is critical for commercializing cellulosic fuel ethanol production. Approaches engineer pentose-fermenting yeasts have required expression of additional genes. We implemented a high-throughput strategy improve anaerobic growth on xylose and rate production by evaluating overexpression each native S. gene collection haploid PJ69–4 MATa strains expressing open reading frames...
In these studies, we pretreated sweet sorghum bagasse (SSB) using liquid hot water (LHW) or dilute H2 SO4 (2 g L-1 ) at 190°C for zero min (as soon as temperature reached 190°C, cooling was started) to reduce generation of sugar degradation fermentation inhibiting products such furfural and hydroxymethyl (HMF). The solids loading were 250-300 . This followed by enzymatic hydrolysis. After hydrolysis, 89.0 sugars, 7.60 acetic acid, 0.33 furfural, 0.07 HMF released. pretreatment hydrolysis...
The yeast Kluyveromyces marxianus is a potential microbial catalyst for fuel ethanol production from wide range of biomass substrates. To improve its growth and yield at elevated temperature under microaerophilic conditions, K. NRRL Y-1109 was irradiated with UV-C using automated protocols on robotic platform picking spreading cultures processing the resulting plates. plates were incubated anaerobic conditions xylose or glucose 5 mo 46 °C. Two mutant strains (designated 7-1 8-1) survived...
In these studies, liquid hot water (LHW) pretreated and enzymatically hydrolyzed Sweet Sorghum Bagasse (SSB) hydrolyzates were fermented in a fed-batch reactor. As reported the preceding paper, culture was not able to ferment hydrolyzate I batch process due presence of high level toxic chemicals, particular acetic acid released from SSB during hydrolytic process. To be obtained 250 g L-1 hydrolysis, reactor with situ butanol recovery devised. The started II when good cell growth vigorous...
A yeast artificial chromosome (YAC) containing a multigene cassette for expression of enzymes that enhance xylose utilization (xylose isomerase [XI] and xylulokinase [XKS]) was constructed transformed into Saccharomyces cerevisiae to demonstrate feasibility as stable protein system in design an assembly process suitable automated platform. Expression XI XKS from the YAC confirmed by Western blot PCR analyses. The recombinant wild-type strains showed similar growth on plates hexose sugars,...
A new quasi-Coulomb relation between magnetic force vs distance, flux density, and pole area is proposed in this paper based on the empirical analysis distance measurements. simple device designed fabricated to measure repulsive/attractive two magnets. The measurement results show that relationship similar Coulomb’s electrostatic law, but attraction greater than repulsion. proportional integral of density with areas pole. This useful evaluate torque speed a rim-driven motor, which plays an...